Co-expression of MtDREB1C and RcXET Enhances Stress Tolerance of Transgenic China Rose (Rosa chinensis Jacq.)

Chen, Ji-Ren; Chen, Yanbin; Ziemiańska, Monika; Liu, Rong; Deng, Zhili; Niedźwiecka-Filipiak, Irena; Liu, Yanlin; Jiao, Jinxia; Xiong, Xingyao

doi:10.1007/s00344-015-9564-z

Cited by 17 publications

(6 citation statements)

References 46 publications

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“…Overexpression of PeXTH from Populus euphratica in tobacco plants increased their capacity for salt and Cd tolerance [ 31 , 32 ]. Overexpression of XTH from rose ( Rosa rugosa ) enhanced drought resistance in transgenic plants [ 33 ]. The T-DNA insertion mutants xth31 , xth17 and xth15 were more aluminum resistant than the wild type in Arabidopsis [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, and phylogenetic and expression profiling analyses, of XTH gene families in Brassica rapa L. and Brassica oleracea L.

Liu

et al. 2020

BMC Genomics

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Background Xyloglucan endotransglucosylase/hydrolase genes (XTHs) are a multigene family and play key roles in regulating cell wall extensibility in plant growth and development. Brassica rapa and Brassica oleracea contain XTHs, but detailed identification and characterization of the XTH family in these species, and analysis of their tissue expression profiles, have not previously been carried out. Results In this study, 53 and 38 XTH genes were identified in B. rapa and B. oleracea respectively, which contained some novel members not observed in previous studies. All XTHs of B. rapa, B. oleracea and Arabidopsis thaliana could be classified into three groups, Group I/II, III and the Early diverging group, based on phylogenetic relationships. Gene structures and motif patterns were similar within each group. All XTHs in this study contained two characteristic conserved domains (Glyco_hydro and XET_C). XTHs are located mainly in the cell wall but some are also located in the cytoplasm. Analyses of the mechanisms of gene family expansion revealed that whole-genome triplication (WGT) events and tandem duplication (TD) may have been the major mechanisms accounting for the expansion of the XTH gene family. Interestingly, TD genes all belonged to Group I/II, suggesting that TD was the main reason for the largest number of genes being in these groups. B. oleracea had lost more of the XTH genes, the conserved domain XET_C and the conserved active-site motif EXDXE compared with B. rapa, consistent with asymmetrical evolution between the two Brassica genomes. A majority of XTH genes exhibited different tissue-specific expression patterns based on RNA-seq data analyses. Moreover, there was differential expression of duplicated XTH genes in the two species, indicating that their functional differentiation occurred after B. rapa and B. oleracea diverged from a common ancestor. Conclusions We carried out the first systematic analysis of XTH gene families in B. rapa and B. oleracea. The results of this investigation can be used for reference in further studies on the functions of XTH genes and the evolution of this multigene family.

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Section: Introductionmentioning

confidence: 99%

Genome-wide identification, and phylogenetic and expression profiling analyses, of XTH gene families in Brassica rapa L. and Brassica oleracea L.

Liu

et al. 2020

BMC Genomics

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“…For example, overexpression of HvXTH1 in barley resulted in the enlargement of the stomata of transgenic plants relative to those of WT plants under drought treatment, indicating that transgenic plants were more drought tolerant ( Fu, 2019 ). Overexpression of XTH genes from rose ( Rosa rugosa ) increased the drought tolerance of transgenic China rose plants ( Chen et al., 2016 ). In addition, the presence of xyloglucan in early land plants suggests that XTH gene family members have played a key role in the transition from wetter to drier habitats ( Popper, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Zong

et al. 2022

Front. Plant Sci.

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Liriodendron chinense is a relic tree species of the family Magnoliaceae with multiple uses in timber production, landscape decoration, and afforestation. L. chinense often experiences drought stress in arid areas. However, the molecular basis underlying the drought response of L. chinense remains unclear. Many studies have reported that the xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in drought stress resistance. Hereby, to explore the drought resistance mechanism of L. chinense, we identify XTH genes on a genome-wide scale in L. chinense. A total of 27 XTH genes were identified in L. chinense, and these genes were classified into three subfamilies. Drought treatment and RT-qPCR analysis revealed that six LcXTH genes significantly responded to drought stress, especially LcXTH21. Hence, we cloned the LcXTH21 gene and overexpressed it in tobacco via gene transfer to analyze its function. The roots of transgenic plants were more developed than those of wild-type plants under different polyethylene glycol (PEG) concentration, and further RT-qPCR analysis showed that LcXTH21 highly expressed in root compared to aboveground organs, indicating that LcXTH21 may play a role in drought resistance through promoting root development. The results of this study provide new insights into the roles of LcXTH genes in the drought stress response. Our findings will also aid future studies of the molecular mechanisms by which LcXTH genes contribute to the drought response.

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“…In this study, co-culture for 3 days had the best effect. We choose 8 mg/L hygromycin, the same as the previous study in Rosa chinensis ( Chen et al, 2016 ). In the early stage after A. tumefaciens , selected high Hygromycin concentration (50 mg/L) to suppress the excessive growth of A. tumefaciens on the callus surface, and the low hygromycin concentration (20 mg/L) did not affect the growth of rose plants in the later stage ( Liu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

et al. 2022

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Calla lily (Zantedeschia spp.) have great aesthetic value due to their spathe-like appearance and richness of coloration. However, embryonic callus regeneration is absent from its current regeneration mechanism. As a result, constructing an adequate and stable genetic transformation system is hampered, severely hindering breeding efforts. In this research, the callus induction effectiveness of calla lily seed embryos of various maturities was evaluated. The findings indicated that mature seed embryos were more suitable for in vitro regeneration. Using orthogonal design experiments, the primary elements influencing in vitro regeneration, such as plant growth regulators, genotypes, and nanoscale materials, which was emergent uses for in vitro regeneration, were investigated. The findings indicated that MS supplemented with 6-BA 2 mg/L and NAA 0.1 mg/L was the optimal medium for callus induction (CIM); the germination medium (GM) was MS supplemented with 6-BA 2 mg/L NAA 0.2 mg/L and 1 mg/L CNTs, and the rooting medium (RM) was MS supplemented with 6-BA 2 mg/L NAA 0.7 mg/L and 2 mg/L CNTs. This allowed us to verify, in principle, that the Agrobacterium tumefaciens-mediated genetic transformation system operates under optimal circumstances using the GUS reporter gene. Here, we developed a seed embryo-based genetic transformation regeneration system, which set the stage for future attempts to create new calla lily varieties.

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Co-expression of MtDREB1C and RcXET Enhances Stress Tolerance of Transgenic China Rose (Rosa chinensis Jacq.)

Cited by 17 publications

References 46 publications

Genome-wide identification, and phylogenetic and expression profiling analyses, of XTH gene families in Brassica rapa L. and Brassica oleracea L.

Genome-wide identification, and phylogenetic and expression profiling analyses, of XTH gene families in Brassica rapa L. and Brassica oleracea L.

Genome-wide identification of XTH genes in Liriodendron chinense and functional characterization of LcXTH21

Establishment of an efficient regeneration and Agrobacterium transformation system in mature embryos of calla lily (Zantedeschia spp.)

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