Activation of ALDH2 attenuates high glucose induced rat cardiomyocyte fibrosis and necroptosis

Kang, Pan; Wang, Jiahui; Fang, Dian Chun; Fang, Tingting; Yu, Ying; Zhang, Weiping; Shen, Lin; Li, Zhenghong; Wang, Hongju; Ye, Hongwei; Gao, Qin

doi:10.1016/j.freeradbiomed.2019.10.416

Cited by 54 publications

(50 citation statements)

References 63 publications

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“…All animal procedures were in accordance with the United States National Institutes of Health Guide and were approved by the Animal Use and Care Committee of PLA Rocket Force Characteristic Medical Center, China. In brief, the primary CMs were isolated from the hearts of SD rats (1–3 days) with sterile conditions as previously described (Kang et al, 2020). Then, cells were cultured in Dulbecco's modified Eagle's medium supplemented with d ‐glucose (5.5 mmol/L), 10% fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 mg/ml).…”

Section: Methodsmentioning

confidence: 99%

“…The cell supernatants were collected, and the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) levels were measured by enzyme-linked immunosorbent assay (ELI-SA) kits according to the manufacturer's introductions (Kang et al, 2020). Superoxide production by dihydroethidium (DHE) staining method as previously described (Luo et al, 2019).…”

Section: Measurement Of Inflammation Factorsmentioning

confidence: 99%

“…China. In brief, the primary CMs were isolated from the hearts of SD rats (1-3 days) with sterile conditions as previously described (Kang et al, 2020). Then, cells were cultured in Dulbecco's modified Eagle's medium supplemented with D-glucose (5.5 mmol/L), 10% fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 mg/ml).…”

Section: Introductionmentioning

confidence: 99%

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Irisin ameliorates high glucose‐induced cardiomyocytes injury via AMPK/mTOR signal pathway

Deng

Zhang

Chen³

et al. 2020

Cell Biology International

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High glucose (HG)-induced cardiomyocytes (CMs) injury is a leading cause of diabetic cardiomyopathy with little treatment options. Irisin, a new myokine, which is cleaved from its precursor fibronectin type III domain-containing protein 5 (FNDC5), has aroused great attention as an essential cardioprotective factor and glucose metabolism regulator but little was known on diabetic cardiomyopathy yet. Here, we aim to clarify the role of irisin in the HG-induced CMs injury. Neonatal Sprague-Dawley rat CMs were cultured in a normal or HG medium for 12, 24, and 48 hr, respectively before exposing to irisin. The apoptosis level was determined by terminal-deoxynucleotidyl transferase-mediated-dUTP nick end-labeling assay. Cell viability was measured with the conventional methyl thiazolyl tetrazolium assay. Moreover, reactive oxygen species production was evaluated by dihydroethidium staining. Inflammatory factors, namely tumor necrosis factor-α, interleukin-6, interleukin-1β were determined by enzyme-linked immunosorbent assay kits. Furthermore, protein and messenger RNA (mRNA) expressions were measured by western blot and quantitative real-time polymerase chain reaction, respectively. HG increases the apoptosis of CMs and activated the inflammatory responses and oxidative stress in CMs. Meanwhile, the mRNA and protein expressions of FNDC5 are decreased after HG exposure. Nevertheless, the increased apoptosis is alleviated by irisin treatment. Notably, irisin suppresses the inflammatory responses and oxidative stress in injured CMs. Mechanically, after the administration of Compound C, AMP-activated protein kinase (AMPK) inhibitor, these cardioprotective effects resulting from irisin are reversed. Irisin plays a significant role in antiapoptosis, antiinflammation, antioxidative stress in HG-induced CMs via AMPK/mammalian target of the rapamycin signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Inflammation Factorsmentioning

confidence: 99%

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Irisin ameliorates high glucose‐induced cardiomyocytes injury via AMPK/mTOR signal pathway

Deng

Zhang

Chen³

et al. 2020

Cell Biology International

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“…This was supported by Kang et al, who treated primary cardiomyocytes with 35 mmol/L glucose for 24 h [108]. The ELISA results showed that the levels of TNF-α, IL-6, and IL-1β and the expression of collagen I and III mRNA were higher than those in the normal control group [108]. These findings suggest that fibrosis is associated with inflammation.…”

Section: Inflammatory Factorsmentioning

confidence: 67%

“…Furthermore, a complex regulatory network consisting of these inflammatory factors activates both myofibroblasts and fibrotic pathological processes [107]. This was supported by Kang et al, who treated primary cardiomyocytes with 35 mmol/L glucose for 24 h [108]. The ELISA results showed that the levels of TNF-α, IL-6, and IL-1β and the expression of collagen I and III mRNA were higher than those in the normal control group [108].…”

Section: Inflammatory Factorsmentioning

confidence: 95%

Roles of Biomarkers in Myocardial Fibrosis

et al. 2020

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Myocardial fibrosis is observed in various cardiovascular diseases and plays a key role in the impairment of cardiac function. Endomyocardial biopsy, as the gold standard for the diagnosis of myocardial fibrosis, has limitations in terms of clinical application. Therefore, biomarkers have been recommended for noninvasive assessment of myocardial fibrosis. This review discusses the role of biomarkers in myocardial fibrosis from the perspective of collagen.

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Lycopene prevents cell death in NRK‐52E cells by inhibition of high glucose‐activated DNA damage and apoptotic, autophagic, and necrotic pathways

Usta,

Yüksek,

Çetin

et al. 2024

J Biochem & Molecular Tox

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This study aims to investigate the effects of lycopene on apoptotic, autophagic, and necrotic pathways, oxidative status, and DNA damage in diabetic nephropathy at the molecular level. The sample of the study includes seven groups: lycopene (L), high glucose (G), high glucose + lycopene (GL), and control (C) groups tested at 12 and 24 h. The expression levels of genes in oxidative, apoptotic, autophagic, and necrotic cell death pathways are determined by reverse transcription‐quantitative polymerase chain reaction analysis. The comet assay method is used for the analysis of DNA damage. It is observed that adding lycopene to high glucose for protective purposes reduces the expression of genes related to apoptosis, autophagy, and necrosis, as well as the DNA damage index, compared to cells given high glucose alone. Lycopene can be a safe and effective alternative agent.

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Activation of ALDH2 attenuates high glucose induced rat cardiomyocyte fibrosis and necroptosis

Cited by 54 publications

References 63 publications

Irisin ameliorates high glucose‐induced cardiomyocytes injury via AMPK/mTOR signal pathway

Irisin ameliorates high glucose‐induced cardiomyocytes injury via AMPK/mTOR signal pathway

Roles of Biomarkers in Myocardial Fibrosis

Lycopene prevents cell death in NRK‐52E cells by inhibition of high glucose‐activated DNA damage and apoptotic, autophagic, and necrotic pathways

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