Activation of AMP-Activated Protein Kinase and Stimulation of Energy Metabolism by Acetic Acid in L6 Myotube Cells

Maruta, Hitomi; Yoshimura, Yukihiro; Araki, Aya; Kimoto, Masumi; Takahashi, Yoshitaka; Yamashita, Hiromi

doi:10.1371/journal.pone.0158055

Cited by 60 publications

(67 citation statements)

References 63 publications

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“…Moreover, the ingested acetate increased the AMP:ATP ratio thereby promoting the phosphorylation of AMPK which regulates numerous enzymes involved with lipid metabolism (Yamashita et al 2009). A recent in vitro study supports the finding that acetate promotes the phosphorylation of AMPK and its downstream metabolic pathways in skeletal muscle (Maruta et al 2016).…”

Section: The Impact Of Scfas On Skeletal Muscle Energy Metabolismmentioning

confidence: 73%

Regulation of energy expenditure and substrate oxidation by short-chain fatty acids

Sukkar

Lett

Frost

et al. 2019

Journal of Endocrinology

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Short-chain fatty acids (SCFAs) are metabolites produced from the fermentation of dietary fibre by the gut microbiota. High-fibre diets have been associated with lower weight gain and a number of reports have therefore investigated if these positive effects of a dietary fibre on body weight can be replicated through the direct administration of SCFAs. Many of these studies have reported that SCFAs can prevent or attenuate long-term body weight gain by increasing energy expenditure through increased lipid oxidation. The aim of the present review is to therefore evaluate the current evidence for an effect of SCFAs on whole-body energy expenditure and to assess the potential underlying mechanisms. The available data highlights that SCFAs can exert multiple effects at various organ and tissue sites that would cumulatively raise energy expenditure via a promotion of lipid oxidation. In conclusion, the present review proposes that dietary interventions and other therapies that augment gut-derived SCFAs and systemic availability may present an effective strategy to improve long-term energy balance and body weight management.

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Section: The Impact Of Scfas On Skeletal Muscle Energy Metabolismmentioning

confidence: 73%

Regulation of energy expenditure and substrate oxidation by short-chain fatty acids

Sukkar

Lett

Frost

et al. 2019

Journal of Endocrinology

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“…Although cecal and colonic SCFA concentrations were not measured in the current study, it was previously demonstrated that in broiler chickens, both synbiotic [26] or probiotic administration [27] led to an increase in cecal acetic, butyric, and propionic acid content. Moreover, recent in vitro and in vivo studies showed that gut microbiota through its fermentation end products, especially acetate and butyrate, may activate the mechanisms involved in regulating energy metabolism in skeletal muscle [28,29]. These effects have been attributed to increased AMP-activated protein kinase (AMPK) phosphorylation, a protein known to be a major cellular energy sensor in the muscle tissue.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics

Dunisławska

Siwek

Sławińska

et al. 2020

Animals

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Simple Summary: Poultry production plays a major role in providing meat products to global markets. Hence, there is a continued interest of researchers in the possibilities of improving the production parameters of broiler chickens. A direct response from muscles and their metabolism to in ovo synbiotic stimulation on day 12 of egg incubation has already been widely documented. However, it is necessary to analyze the molecular mechanisms determining the phenotypic effects. The present research aimed to explain the molecular background of the quality of broiler chicken meat after injection of synbiotics based on Lactobacillus strains into the air chamber of the egg. Characterization of the meat quality is based on the signature of the metabolic gene expression closely related to muscles and basic physiological parameters. Abstract:To better understand the effects of synbiotics administered at early stages of embryonic development in poultry, it is necessary to analyze direct effects (meat quality) and the molecular background. The molecular interpretation of poultry meat properties after in ovo administration of synbiotics remains to be reported. The purpose of the present study was to analyze the molecular background of meat quality based on gene expression and basic physiological parameters. Eggs were injected with (S1) Lactobacillus salivarius with galacto-oligosaccharides or (S2) Lactobacillus plantarum with raffinose family oligosaccharides. The pectoral muscle was collected at two time points (day 7 and day 42) and subjected to RNA isolation. Gene expression analysis was performed by RT-qPCR for a panel of eight genes associated with metabolism. The concentration of glucose and hormones (insulin, glucagon, and leptin (S1 p = 0.04)) was also increased. The obtained results showed that metabolic gene expression in the muscle was more differential due to synbiotic stimulation on day 7 (FST in S1 p = 0.03; PDK4 in S1 p = 0.02 and S2 p = 0.01; CEBPB in S1 p = 0.01 and S2 p = 0.008; PHKB in S1 p = 0.01; PRKAG3 in S1 p = 0.02) than on day 42 (PDK4 in S1 p = 0.04). On the basis of the results obtained, it can be concluded that in ovo stimulation with S1 triggered the most potent and favorable changes in the pectoral muscle gene expression in broiler chickens. Author Contributions: Conceptualization, A.D., M.S.; methodology, A.D; formal analysis, A.D., A.S.; investigation, A.D.; resources, M.S., data curation, A.D., A.L., A.H., P.A.K.; writing-original draft preparation, A.D., A.L., A.H.; writing-review and editing, M.S., A.S.; visualization, A.D.; supervision, M.B., M.S., project administration, M.B., funding acquisition, M.B., A.D. All authors have read and agreed to the published version of the manuscript.

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“…L6 cells were cultured as previously described [9]. L6 myoblasts were grown in DMEM containing 10% (v/v) FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin in 5% CO 2 at 37˚C.…”

Section: L6 Cell Culture and Sirna Transfectionmentioning

confidence: 99%

“…It was demonstrated in our previous study that acetic acid stimulates the AMPK via an increase in the AMP/ATP ratio through its metabolic pathway, induces myoglobin and glucose transporter 4 (GLUT4) expressions, stimulates glucose incorporation, and suppresses lipid accumulation in the L6 skeletal muscle cells [6,9].…”

Section: Introductionmentioning

confidence: 99%

Acetic acid stimulates G-protein-coupled receptor GPR43 and induces intracellular calcium influx in L6 myotube cells

Maruta

Yamashita

2020

PLoS ONE

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Short chain fatty acids (SCFAs) produced endogenously in the gut by bacterial fermentation of dietary fiber have been studied as nutrients that act as signaling molecules to activate Gprotein coupled receptors (GPCRs) such as GPR41 and GPR43. GPR43 functioning involves the suppression of lipid accumulation and maintaining body energy homeostasis, and is activated by acetic acid or propionic acid. Previously, we reported that the orally administered acetic acid improves lipid metabolism in liver and skeletal muscles and suppresses obesity, thus improving glucose tolerance. Acetic acid stimulates AMP-activated protein kinase (AMPK) through its metabolic pathway in skeletal muscle cells. We hypothesized that acetic acid would stimulate GPR43 in skeletal muscle cells and has function in modulating gene expression related to muscle characteristics through its signal pathway. The objective of the current study was to clarify this effect of acetic acid. The GPR43 expression, observed in the differentiated myotube cells, was increased upon acetic acid treatment. Acetic acid induced the intracellular calcium influx in the cells and this induction was significantly inhibited by the GPR43-specific siRNA treatment. The calcineurin molecule is activated by calcium/calmodulin and is associated with proliferation of slow-twitch fibers. Calcineurin was activated by acetic acid treatment and inhibited by the concomitant treatment with GPR43-siRNA. Acetic acid induced nuclear localization of myocyte enhancer factor 2A (MEF2A), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), and nuclear factor of activated t cells c1 (NFATc1). However, these localizations were abolished by the treatment with GPR43-siRNA. It was concluded that acetic acid plays a role in the activation of GPR43 and involves the proliferation of slow-twitch fibers in L6 skeletal muscles through the calcium-signaling pathway caused by induction of intracellular calcium influx.

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Activation of AMP-Activated Protein Kinase and Stimulation of Energy Metabolism by Acetic Acid in L6 Myotube Cells

Cited by 60 publications

References 63 publications

Regulation of energy expenditure and substrate oxidation by short-chain fatty acids

Regulation of energy expenditure and substrate oxidation by short-chain fatty acids

Metabolic Gene Expression in the Muscle and Blood Parameters of Broiler Chickens Stimulated In Ovo with Synbiotics

Acetic acid stimulates G-protein-coupled receptor GPR43 and induces intracellular calcium influx in L6 myotube cells

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