Activation of an immune response in Litopenaeus vannamei by oral immunization with phagocytosis activating protein (PAP) DNA

Khimmakthong, Umaporn; Kongmee, Pataraporn; Deachamag, Panchalika; Leggat, Ureporn; Chotigeat, Wilaiwan

doi:10.1016/j.fsi.2013.01.004

Cited by 12 publications

(7 citation statements)

References 62 publications

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“…This gene is located at the ribosomal subunit interface of the 60S subunit inside the cell (94). Interestingly, several studies have demonstrated the role of the rpl26 gene as a phagocytosisactivating protein, thus being highly involved in the immune response, since phagocytosis is a major mechanism used to remove pathogens and cell debris (95)(96)(97). Furthermore, it has been possible to demonstrate that the rpl26 gene has a strong ability to bind p53 mRNA and thereby to stimulate p53 translation, as previously indicated (98,99).…”

Section: Inflammatory Response and Immune Signalingmentioning

confidence: 99%

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

et al. 2021

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In the present study, the modulation of the transcriptional immune response (microarray analysis) in the head kidney (HK) of the anadromous fish Atlantic salmon (Salmo salar) fed a diet supplemented with an olive fruit extract (AQUOLIVE®) was evaluated. At the end of the trial (133 days), in order to investigate the immunomodulatory properties of the phytogenic tested against a bacterial infection, an in vivo challenge with Aeromonas salmonicida was performed. A total number of 1,027 differentially expressed genes (DEGs) (805 up- and 222 downregulated) were found when comparing the transcriptomic profiling of the HK from fish fed the control and AQUOLIVE® diets. The HK transcripteractome revealed an expression profile that mainly favored biological processes related to immunity. Particularly, the signaling of i-kappa B kinase/NF-kappa and the activation of leukocytes, such as granulocytes and neutrophils degranulation, were suggested to be the primary actors of the innate immune response promoted by the tested functional feed additive in the HK. Moreover, the bacterial challenge with A. salmonicida that lasted 12 days showed that the cumulative survival was higher in fish fed the AQUOLIVE® diet (96.9 ± 6.4%) than the control group (60.7 ± 13.5%). These results indicate that the dietary supplementation of AQUOLIVE® at the level of 0.15% enhanced the systemic immune response and reduced the A. salmonicida cumulative mortality in Atlantic salmon smolts.

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Section: Inflammatory Response and Immune Signalingmentioning

confidence: 99%

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

et al. 2021

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“…Injection with the PAP gene was also found to significantly increase the percentage of phagocytosis and the phagocytic index [26]. In another investigation, oral administration of the chitosan-PAP-phMGFP gene was applied to determine the ability of the gene to induce shrimp immunity [27].…”

Section: Discussionmentioning

confidence: 99%

“…, and L. vannamei [27] against pathogens. We prepared RPS3a-phMGFP in the form of chitosan-DNA nanoparticles and fed to the shrimp.…”

Section: P Monodonmentioning

confidence: 99%

Effects of ribosomal protein S3a (RPS3a) on the survival of WSSV-infected pacific white shrimp (Litopenaeus vannamei)

Iatsui¹,

Chotigeat²,

Deachamag³

2020

ScienceAsia

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White spot syndrome virus (WSSV) causes substantial economic losses in shrimp farming. It has been reported that shrimp infected with this virus exhibits an overexpression of ribosomal protein S3a (RPS3a). Therefore, a recombinant protein, His-RPS3a, from shrimp was produced and its potential to protect shrimp from WSSV was evaluated. The purified His-RPS3a was injected at concentrations of 1-20 µg/shrimp to determine the expression of the genes prophenoloxidase (proPO) and phagocytosis-activating protein (PAP) by real-time PCR. PAP expression increased 4.1-fold when His-RPS3a was injected at 1 µg/shrimp, and proPO gene expression increased 5.3-fold when His-RPS3a was injected at 20 µg/shrimp. Therefore, shrimp were injected with His-RPS3a at concentrations of 10, 20, or 40 µg/shrimp for 3 days and then challenged with WSSV. The relative percentages of survival (RPS) of the WSSV-challenged shrimp after injection with the His-RPS3a were 23%, 27%, and 35%, respectively. Besides, RPS3a-phMGFP was prepared in the form of chitosan-DNA nanoparticles and fed at 75 µg/shrimp/day for 7 days. PAP expression was found to be higher than that in the control group. Furthermore, shrimp fed with RPS3a-phMGFP DNA at 75 µg/shrimp/day were challenged with WSSV and further cultured for 15 days. The RPS of the WSSV-challenged shrimp after feeding with RPS3a-phMGFP was 35%, whereas the control shrimp exhibited 100% death. These results demonstrated that RPS3a acts as an antigen and could delay the death of WSSV-infected shrimp by activating the general immune system; furthermore, RPS3a may be activated after WSSV infection to induce the production of proinflammatory cytokines.

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“…The use of probiotics or immunostimulants in shrimp aquaculture by feeding dietary components, such as β‐1,3‐glucan (Chang, Su, Chen & Liao ), polysaccharide gel (Pholdaeng & Pongsamart ), chitosan‐PAP (Khimmakthong, Kongmee, Deachamag, Leggat & Chotigeat ) and fortilin (Tonganunt, Nupan, Saengsakda, Suklour, Wanna, Senapin, Chotigeat & Phongdara ), can effectively induce the expression of immune‐related genes and so lead to greater disease resistance in shrimp. By feeding shrimp with a rALF Pm 3‐supplemented diet, the shrimp survival rate was increased upon WSSV challenge to 35–36% compared to the 0% for the control group from 13 to 15 dpi.…”

Section: Discussionmentioning

confidence: 99%

A potential application of shrimp antilipopolysaccharide factor in disease control in aquaculture

et al. 2015

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Antilipopolysaccharide factor isoform 3 from Penaeus monodon (ALFPm3) exhibits broad antimicrobial activities against various microbes, including bacteria, fungi and viruses, suggesting its potential use in disease control in shrimp aquaculture. Herein, the recombinant ALFPm3 protein (rALFPm3) exhibited effective antibacterial action against Vibrio parahaemolyticus, the causative agent of the new severe emerging disease, early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) at the minimum inhibitory concentration value of 1.25-5.00 lM.The morphological changes such as bleb formation and cell distortion of V. parahaemoluticus were introduced after rALFPm3 treatment. Furthermore, to control V. parahaemoluticus infection, shrimp were treated with rALFPm3 by immersion and reduction in the cumulative mortality of V. parahaemoluticus-infected Penaeus vannamei was observed. As a potential immunostimulant, the administration of dietary rALFPm3 enhanced the transcript level of five immune-related genes; ALFPm3, haemocyte homoeostasis-associated protein, phagocytosis-activating protein, prophenoloxidase-activating enzyme 1 and cytosolic manganese superoxide dismutase. In addition, dietary rALFPm3 increased the shrimp survival after white spot syndrome virus (WSSV) injection by >35% compared to the 0% survival for the control group. Taken together, these results indicate the potential use of dietary rALFPm3 as immunostimulant in controlling AHPND and WSSV infections in shrimp aquaculture.

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Activation of an immune response in Litopenaeus vannamei by oral immunization with phagocytosis activating protein (PAP) DNA

Cited by 12 publications

References 62 publications

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

A Bioactive Extract Rich in Triterpenic Acid and Polyphenols from Olea europaea Promotes Systemic Immunity and Protects Atlantic Salmon Smolts Against Furunculosis

Effects of ribosomal protein S3a (RPS3a) on the survival of WSSV-infected pacific white shrimp (Litopenaeus vannamei)

A potential application of shrimp antilipopolysaccharide factor in disease control in aquaculture

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