Activation of C57BL/6 Spleen Cells by the Mitogenic Principle Derived from Mycoplasma Arthritidis

Giebler, D.; Brehm, G.; Nicklas, Werner; Kirchner, Holger

doi:10.1016/s0171-2985(86)80017-1

Immunobiology

1986

DOI: 10.1016/s0171-2985(86)80017-1

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Activation of C57BL/6 Spleen Cells by the Mitogenic Principle Derived from Mycoplasma Arthritidis

D. Giebler¹,

G. Brehm²,

Werner Nicklas³

et al.

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1986

1990

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Cited by 5 publications

(2 citation statements)

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“…Material with similar activity was isolated from the same organism by Kirchner and co-workers, who described its T-cell mitogenicity (15) and potential to induce interferon-T in leukocyte cultures (14). The activity of this factor was found to be genetically restricted to certain mouse strains (7,11). Mitogenicity is not confined to products from M. arthritidis (4).…”

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Mycoplasma fermentans-derived high-molecular-weight material induces interleukin-6 release in cultures of murine macrophages and human monocytes

et al. 1990

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A Mycoplasma fermentans-derived high-molecular-weight material (MDHM) is described which causes differentiation of concanavalin A-stimulated CBA/J or C57BL/6 mouse thymocytes to cytolytic effector T cells (CTLs). The effect of MDHM was inhibited by addition of monoclonal anti-interleukin-6 (IL-6) antibody. It could also be abolished after removal of adherent cells. However, adherent cell-depleted thymocytes could still form CTLs after addition of IL-6. The action of MDHM could thus be explained by the capacity of MDHM to stimulate IL-6 release from adherent cells. MDHM was active on macrophages from CBA/J and C3H/HeJ endotoxin nonresponder mice and was also capable of stimulating IL-6 release from human monocytes. On gel chromatography, MDHM had an apparent molecular size of 1.5 x 106 daltons. Treatment with RNase and DNase had no effect on either size or biological activity. Proteinase K did not abolish activity but reduced the apparent molecular size of MDHM. MDHM production by M. fermentans required either coculture with eucaryotic cell lines in RPMI 1640 medium with fetal calf serum or addition of eucaryotic cell sonic extracts to this medium. The biological activity of MDHM is not identical to that of a mitogen for murine spleen cells derived from M. arthritidis; MDHM caused only slight proliferation in this system compared with the mitogen from M. arthritidis, and the latter did not elicit IL-6 release from macrophages. The results are discussed in relation to mycoplasmas as putative etiological agents for rheumatoid arthritis, since high IL-6 titers were reported for synovial fluid from patients with this disease.

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Mycoplasma fermentans-derived high-molecular-weight material induces interleukin-6 release in cultures of murine macrophages and human monocytes

et al. 1990

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“…We have confirmed and extended their experiments and from the analysis of a variety of different mycoplasma strains, it appeared that production of MAS activity was unique to 41 Mycoplasma arthritidis and was not found with other mycoplasma strains. Previously our work has been concerned with lymphocyte cultures derived from mouse spleens [8,[11][12][13]. It is the purpose of this report to summarize the data that we obtained with human leucocyte cultures.…”

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Lymphocyte Activation and Induction of Interferon Gamma in Human Leucocyte Cultures by the Mitogen in Mycoplasma arthritidis Supernatant (MAS)

et al. 1986

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This report succinctly summarizes a number of experiments in which human leucocytes were activated by the mitogen in the supernatant of cultured Mycoplasma arthritidis (MAS). Lymphocytes from a large number of donors were found to be reactive when the two parameters of lymphoproliferation and of production of gamma interferon were analysed. Lymphocytes of cord blood origin were also reactive in lymphoproliferation. Their interferon production was low, but this has been established previously for mitogens in general. During the course of the studies, different batches of MAS were tested, including most recently a purified preparation. All of these were active. Removal of accessory cells adherent to nylon wool column abolished MAS reactivity, whereas it has little effect on lymphoproliferation induced by phytohaemagglutinin (PHA). MAS reactivity was also strongly reduced when monoclonal anti-HLA-DR antibodies were added to the leucocyte cultures.

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Mycoplasma-derived leukocyte mitogens

Butler

Kotani

McGarrity

1987

Clinical Immunology Newsletter

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Activation of C57BL/6 Spleen Cells by the Mitogenic Principle Derived from Mycoplasma Arthritidis

Cited by 5 publications

References 10 publications

Mycoplasma fermentans-derived high-molecular-weight material induces interleukin-6 release in cultures of murine macrophages and human monocytes

Mycoplasma fermentans-derived high-molecular-weight material induces interleukin-6 release in cultures of murine macrophages and human monocytes

Lymphocyte Activation and Induction of Interferon Gamma in Human Leucocyte Cultures by the Mitogen in Mycoplasma arthritidis Supernatant (MAS)

Mycoplasma-derived leukocyte mitogens

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