Activation of Caspase-3 in Molt4 Cells by Apoptosis-Inducing Nucleosides from CD57+HLA-DRbright Natural Suppressor Cell Line

Human T Cell Leukemia Cell Death by Apoptosis‐inducing Nucleosides from CD57+ HLA‐DRbright Natural Suppressor Cell Line

“…This result confirmed the involvement of caspase-3, which is activated by sequential proteolytic events that cleave the 32-kDa precursor at aspartic acid residues to generate an active heterodimer of 20-and 12-kDa subunits, 45) corresponding to our recent findings. 46) The active caspase-3, in turn, cleaves the inhibitor of caspase-activated DNase (ICAD) and then activates the caspase-activated DNase (CAD), which migrates to the nucleus and degrades DNA. 47) We consider that AINs are good candidates for anticancer agents, and we are planning further studies on AINs-induced apoptosis in various malignant target cells.…”

Induction of apoptosis mediated by Fas receptor and activation of caspase-3 in MRL-+/+ or MRL-lpr/lpr murine oocytes

“…The oocytes were previously stimulated by anti-Fas mAb (1 µg/ml) at 37 °C for 6 h. A single cell assay that relies on the liberation of AMC (7-amino-4-methylcoumarin) cleaved from the caspase-3 substrate peptide DEVD (Asp-Glu-Val-Asp) (Li et al, 2000) was applied for morphological detection of caspase-3 activity in oocytes. The caspase-3 substrate AMC-conjugated DEVD prepared according to the manufacturer's protocol (PharMingen) was added to a suspension of oocytes and incubated for 2 h. The oocytes were fixed in 2% paraformaldehyde-PBS for 30 min, followed by a permeabilisation step of incubation in 0.2% Triton X-100-PBS for 10 min.…”