Activation of colanic acid biosynthesis linked to heterologous expression of the polyhydroxybutyrate pathway in Escherichia coli

Wu, Haiying; Chen, Shangwei; Ji, Mengyao; Qiao, Chuanling; Shi, Jiping; Sun, Junsong

doi:10.1016/j.ijbiomac.2019.02.004

Cited by 22 publications

(24 citation statements)

References 48 publications

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“…6f ). However, we could only detect colanic acid production when wild-type BW25113 strain was grown under nutrient limiting conditions at low temperature 39 and not when strains were grown on LB agar at 37 °C.…”

Section: Resultsmentioning

confidence: 91%

Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Corsini

Wang

Rehman

et al. 2022

npj Biofilms Microbiomes

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Escherichia coli is a Gram-negative bacterium that colonises the human intestine and virulent strains can cause severe diarrhoeal and extraintestinal diseases. The protein SslE is secreted by a range of pathogenic and commensal E. coli strains. It can degrade mucins in the intestine, promotes biofilm maturation and it is a major determinant of infection in virulent strains, although how it carries out these functions is not well understood. Here, we examine SslE from the commensal E. coli Waksman and BL21 (DE3) strains and the enterotoxigenic H10407 and enteropathogenic E2348/69 strains. We reveal that SslE has a unique and dynamic structure in solution and in response to acidification within mature biofilms it can form a unique aggregate with amyloid-like properties. Furthermore, we show that both SslE monomers and aggregates bind DNA in vitro and co-localise with extracellular DNA (eDNA) in mature biofilms, and SslE aggregates may also associate with cellulose under certain conditions. Our results suggest that interactions between SslE and eDNA are important for biofilm maturation in many E. coli strains and SslE may also be a factor that drives biofilm formation in other SslE-secreting bacteria.

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Section: Resultsmentioning

confidence: 91%

Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Corsini

Wang

Rehman

et al. 2022

npj Biofilms Microbiomes

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“…This operon is responsible of the uptake and processing of the quorum sensing autoinducer-2 (AI-2) molecule that controls genes involved in virulence factors ( Pereira et al 2013 ). Notably, operons involved in bacterial virulence mechanisms were significantly downregulated, including the locus of enterocyte effacement ( lee1-7 ) ( Gaytán et al 2016 ), the type three secretion system apparatus and effectors gene cluster ( TTSS-2 ; Zhou et al 2014 ), the chemotaxis and motility ( che-mot ) operon ( Ditty et al 1998 ), the flagellar ( flg and fli ; Liu and Ochman 2007 ) and fimbrial ( fim ) formation operons, and the CA gene cluster ( wca ; Wu et al 2019 ) ( Figure 6 ). Interestingly, virulence factors of O157:H7 ( i .…”

Section: Resultsmentioning

confidence: 99%

Dual transcriptomic analysis reveals metabolic changes associated with differential persistence of human pathogenic bacteria in leaves of Arabidopsis and lettuce

Jacob

Velásquez

Josh

et al. 2021

G3 Genes|Genomes|Genetics

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Understanding the molecular determinants underlying the interaction between the leaf and human pathogenic bacteria is key to provide the foundation to develop science-based strategies to prevent or decrease the pathogen contamination of leafy greens. In this study, we conducted a dual RNA-sequencing analysis to simultaneously define changes in the transcriptomic profiles of the plant and the bacterium when they come in contact. We used an economically relevant vegetable crop, lettuce (Lactuca sativa L. cultivar Salinas), and a model plant, Arabidopsis thaliana Col-0, as well as two pathogenic bacterial strains that cause disease outbreaks associated with fresh produce, Escherichia coli O157: H7 and Salmonella enterica serovar Typhimurium 14028 s (STm 14028 s). We observed commonalities and specificities in the modulation of biological processes between Arabidopsis and lettuce and between O157: H7 and STm 14028 s during early stages of the interaction. We detected a larger alteration of gene expression at the whole transcriptome level in lettuce and Arabidopsis at 24 hours post inoculation with STm 14028 s compared to that with O157: H7. In addition, bacterial transcriptomic adjustments were substantially larger in Arabidopsis than in lettuce. Bacterial transcriptome was affected at a larger extent in the first 4 hours compared to the subsequent 20 hours after inoculation. Overall, we gained valuable knowledge about the responses and counter-responses of both bacterial pathogen and plant host when these bacteria are residing in the leaf intercellular space. These findings and the public genomic resources generated in this study are valuable for additional data mining.

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“…The repeating unit of colanic acid is comprised of D-galactose, L-fucose, D-glucose, and D-glucuronic acid, which exist in a molar ratio of 2:2:1:1, and colanic acid is further modified with pyruvate substituents and nonstoichiometric acetyl groups [ 4 , 5 , 6 , 7 ]. As a polysaccharide, the molecular weight of colanic acid is variable, and its weight is more than ten million Daltons [ 8 ]. Due to its high molecular weight and numerous hydrophilic groups on the surface, colanic acid has excellent water retention; thus, it has widespread applications in cosmetics [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…As a polysaccharide, the molecular weight of colanic acid is variable, and its weight is more than ten million Daltons [ 8 ]. Due to its high molecular weight and numerous hydrophilic groups on the surface, colanic acid has excellent water retention; thus, it has widespread applications in cosmetics [ 8 ]. In addition to this property, colanic acid has important value in health care products.…”

Section: Introductionmentioning

confidence: 99%

Combinatorial Metabolic Engineering and Enzymatic Catalysis Enable Efficient Production of Colanic Acid

Liu

et al. 2022

Microorganisms

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Colanic acid can promote the lifespan of humans by regulating mitochondrial homeostasis, and it has widespread applications in the field of health. However, colanic acid is produced at a low temperature (20 °C) with low titer. Using Escherichia coli K-12 MG1655, we constructed the SRP-4 strain with high colanic acid production at 30 °C by enhancing the precursor supply and relieving the regulation of transcription for colanic acid synthesis genes by the RCS system. After media optimization, the colanic acid titer increased by 579.9-fold and reached 12.2 g/L. Subsequently, we successfully purified the colanic acid hydrolase and reduced the molecular weight of colanic acid (106.854 kDa), thereby eliminating the inhibition of high-molecular-weight colanic acid on strain growth. Finally, after adding the colanic acid hydrolase (4000 U/L), the colanic acid with low molecular weight reached 24.99 g/L in 3-L bioreactor, the highest titer reported so far. This high-producing strain of colanic acid will promote the application of low-molecular-weight colanic acid in the field of health.

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Activation of colanic acid biosynthesis linked to heterologous expression of the polyhydroxybutyrate pathway in Escherichia coli

Cited by 22 publications

References 48 publications

Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Dual transcriptomic analysis reveals metabolic changes associated with differential persistence of human pathogenic bacteria in leaves of Arabidopsis and lettuce

Combinatorial Metabolic Engineering and Enzymatic Catalysis Enable Efficient Production of Colanic Acid

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