Activation of corneal stromal cells to take up the vital dye neutral red

Weimar, Virginia

doi:10.1016/0014-4827(59)90286-1

Cited by 28 publications

(6 citation statements)

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“…Inhibition of this protease activity blocks these events, but all can be restored in the wounded, protease-inhibited cornea by the topical application of trypsin (Weimar, 1960). Subsequently we showed that trypsin activates the uptake of neutral red by stromal cells in vitro but that without epithelium no activation occurs (Weimar, 1959). Crushing the epithelium immediately activated neutral red uptake by stromal cells (Weimar, 1959).…”

mentioning

confidence: 97%

“…Subsequently we showed that trypsin activates the uptake of neutral red by stromal cells in vitro but that without epithelium no activation occurs (Weimar, 1959). Crushing the epithelium immediately activated neutral red uptake by stromal cells (Weimar, 1959).…”

mentioning

confidence: 97%

“…The cornea provides an accessible model system for studying the cooperative roles of various cell types and the mechanisms of their activation during wound healing. Within 1 hour after injury of rat corneas in vivo there is an activation or release of an apparent protease activity that appears to "trigger" the following: a) corneal invasion by polymomhonuclear leukocytes within 4-6 hours of injury (Weimar, 1957), b) activation of stromal cells to take up neutral red beginning 4 hours after injury (Weimar, 1958(Weimar, , 1959, and c) fibroblast formation at the wound edge in the stroma (Weimar, 1960). Inhibition of this protease activity blocks these events, but all can be restored in the wounded, protease-inhibited cornea by the topical application of trypsin (Weimar, 1960).…”

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confidence: 99%

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Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Haraguchi

Knox

Weimar

et al. 1982

Journal Cellular Physiology

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Recycled mesodermal growth factor (R-MGF) is a pool of proteins of 26,000 molecular weight obtained by recycling gel chromatography of male murine submaxillary gland extracts. R-MGF strikingly accelerates corneal stromal wound healing in vivo, fibroblast growth and migration in cultured corneal buttons and is showing here to stimulate stromal fibroblast growth and division in tissue culture. Chromatographic fractionation of R-MGF has yielded several components, none of which has a greater biological potency than the parent R-MGF. In contrast, two components, MGF-I and -II, when recombined synergistically stimulate fibroblast response in tissue culture and organ culture in excess of those obtained with the parent R-MGF. Three MGF components (I, III, and IV) have been purified and are inactive at 10-15 micrograms/ml in organ culture but potently stimulate fibroblast responses when combined in pairs containing 7.5 micrograms of each component. The striking synergism in organ culture suggests that the stimulation of wound healing by R-MGF in vivo may also reflect synergistic action of more than one R-MGF component. Procedures for isolating gram quantities of R-MGF and for the purification of different R-MGF components by ion exchange chromatography are detailed.

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Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Haraguchi

Knox

Weimar

et al. 1982

Journal Cellular Physiology

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“…This loss did not occur, however, if the epithelium was removed by a different procedure. Following a suggestion of Dr. V. Weimar, the cornea, while still in the eyeball, was held for about 15 seconds with minimal pressure against a camel's-hair brush with the hair clipped to a length of 4 mm and rotating at about 400 RPM (4). No further epithelial material could be removed with a scalpel after this brushing procedure.…”

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Atp Level, Cell Injury, and Apparent Epithelium-Stroma Interaction in the Cornea

Herrmann

Lebeau

1962

The Journal of Cell Biology

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“…According to Weimar [1959Weimar [ , 1962, the epithelium releases factors acti vating the corneal stromal cells to transport the vital dye neutral red. Other studies [Herrmann and Love, 1959;Smelser, 1960] demonstrate that much less 35S inorganic sulfate or glycine-1-UC are incorporated into the corneal stroma underlying an epithelium-free area.…”

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confidence: 99%

The Epithelium in Corneal Wound Healing

Gnädinger¹,

Refojo²,

Itoi³

et al. 1971

Ophthalmic Res

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Tritiated proline was administered to eyes with perforating corneal incisions 5–9 days after the incision to evaluate the biosynthesis of collagen under different experimental conditions. The radioactivity of the corneal tissue was counted after an exposure of 5 h to the isotope. There was significantly less proline uptakein the wounded corneas in which the epithelium was scraped and kept away after wounding either with adhesive barriers or glued-on lenses, as compared to wounds with the epithelium left undisturbed. No decrease of proline incorporation occurred when the scraped epithelium was allowed to grow back, indicating that the trauma of scraping per se was not responsible for the abolishment of the proline uptake. Scraping the epithelium after the exposure to proline led to a lesser decrease of the radioactivity, indicating that the epithelium participated actively in the biosynthesis of stromal collagen. The fact that the epithelium itself was heavily labeled with isotope strengthens the hypothesis of the epithelium being directly involved in the production of collagen or its precursors.

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Activation of corneal stromal cells to take up the vital dye neutral red

Cited by 28 publications

References 11 publications

Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands

Atp Level, Cell Injury, and Apparent Epithelium-Stroma Interaction in the Cornea

The Epithelium in Corneal Wound Healing

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