Activation of gene expression of the O6-methylguanine-DNA-transferase repair enzyme upon the influence of EMAP II cytokine in human cells in vitro

Influence of some biologically active substances on amount of MGMT and MARP proteins in human cells in vitro

“…We used the chemical agents 5-azacytidine and mitomycin C. The conditions of cell treatment with the biologically active substances in the serum-free culture medium were described previously [17].…”

“…1). It should be noted that in our previous works MARP was named as a modified form of MGMT, while the classic 24 kDa protein was named as an unmodified form of MGMT [17,23]. We proposed several hypotheses about the nature of MARP, namely, post-translation modifications, dimerization of MGMT, etc., which have been discussed in the mentioned articles.…”

Influence of EMAP II, IFN-α2b and its medicinal preparations on the MGMT protein amount in human cells in vitro

“…However, in our previous works the Western blot analysis with monoclonal anti-MGMT antibodies, clone 23.2, revealed two highly specific immunoreactive bands: 24 kDa (classic MGMT protein) and 48 kDa (anti-Methyltransferase Antibody Recognizable Protein or MARP) [12,15]. In those works the MARP nature and its induction by exogenous cytokines in human cells in vitro have been discussed.…”

Regulation of mutagenesis by exogenous biological factors in the eukaryotic cell systems

“…However, in both human and mouse cells, we found one general tendency: increasing level of MGMT gene expression in the period of structural instability, and then gradual decreasing in its expression, during the stabilization stage. After long cultivation of the human cell line 4BL it was impossible to reveal either mRNA, or MGMT protein in its regular form (24 kDà) although there was constantly present a protein with a larger molecular weight (50 kDà) which was recognized by specific monoclonal antibodies [30][31][32]. A decrease in a level of the expression of MGMT might be caused by methylation of the corresponding promotor and/or body of this gene.…”

“…Lectins, unlike oncoviruses, stimulated apoptosis in non-permissive cell systems. Besides, it was shown that lectins and cytokine EMAP II strengthened the expression of proteins which were recognized by monoclonal anti-MGMT antibodies [30][31][32]. Thus, these kinds of proteins might be used for strengthening repair system which removes alkyl groups from cellular DNA.…”