Activation of hamster zona-free oocytes by homologous and heterologous spermatozoa

Maleszewski, Marek; Kline, Douglas; Yanagimachi, Ryuzo

doi:10.1530/jrf.0.1050099

Cited by 37 publications

(29 citation statements)

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“…Immediately following sperm attachment, oocytes were continuously monitored. Ca 2ϩ oscillations of oocytes induced by epididymal spermatozoa in golden hamster have been described elsewhere (Swann, 1990;Miyazaki et al, 1992;Maleszewski et al, 1997). In the present study, epididymal spermatozoa induced Ca 2ϩ oscillations in 65.4% (17 of 26) of the oocytes (Table II).…”

Section: Ca 2⍣ Oscillations Induced By Epididymal or Ejaculated Spermsupporting

confidence: 73%

Male accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters

Cheung²

1999

Molecular Human Reproduction

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To evaluate the effect of male accessory sex gland secretions on Ca 2⍣ oscillations of oocytes, epididymal or ejaculated spermatozoa recovered from uteri were used to inseminate oocytes. Ca 2⍣ oscillations were measured by Fura 2 fluorescence imaging (F340/F380). We showed that although Ca 2⍣ oscillations induced by ejaculated spermatozoa had a pattern similar to those induced by epididymal spermatozoa, the amplitude of the first Ca 2⍣ transient in the former group was significantly higher (P < 0.05) and the duration was significantly longer (P < 0.01). Oocytes inseminated with ejaculated spermatozoa recovered from uteri from males had ampullary glands or ventral prostates removed showed significantly lower Ca 2⍣ oscillations compared to the controls (P < 0.05, P < 0.01 respectively). Moreover, the relative area of the first Ca 2⍣ transient in treatment groups was significantly smaller than the control. In addition, a significantly higher percentage of oocytes (52%) inseminated by spermatozoa from males with all accessory sex glands removed showed non-oscillatory Ca 2⍣ transients, compared to the controls (5%, P < 0.05). These results indicate that accessory sex gland secretions can affect Ca 2⍣ oscillations. The differences between Ca 2⍣ oscillations induced by epididymal and uterine spermatozoa from males with all accessory sex glands removed suggest that uterine factors may also influence this process.

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Section: Ca 2⍣ Oscillations Induced By Epididymal or Ejaculated Spermsupporting

confidence: 73%

Male accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters

Cheung²

1999

Molecular Human Reproduction

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“…Our results coincide with the finding of Malezewenski et al [23], who reported on the activation of oocytes after sperm penetration. Their study was conducted with homologous and heterologous spermatozoa and zona pellucida-free hamster oocytes.…”

Section: Discussionsupporting

confidence: 93%

“…It is now well established that the pronucleus formation and second polar body extrusion in fertilized eggs and parthenogenetically activated oocytes can be induced via the inactivation of MPF [10,11,[22][23][24]. Recently, the inactivation of MAP kinase induced long after inactivation of MPF, has been shown to be required for pronucleus formation and second polar body extrusion [13].…”

Section: Discussionmentioning

confidence: 99%

Activation by Stimuli of Sperm Penetration in Bovine Oocytes Treated with Higher Concentration of BAPTA/AM.

2000

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Abstract:We attempted to determine whether [Ca 2+ ]i elevation in penetrated bovine oocytes is essential for the activation events, including inactivation of MPF and MAP kinase, pronucleus formation, and second polar body extrusion. After maturing for 20 h, oocytes were pretreated with various concentrations of BAPTA/AM for 1 h, coincubated with sperm for 7 h and cultured for 17 h. Percentages of polyspermy increased with BAPTA/AM concentration, and nearly all of the penetrated oocytes that had been pretreated with more than 25 µM BAPTA/ AM were penetrated by two or more spermatozoa. In oocytes pretreated with higher concentrations of BAPTA/ AM (25 and 50 µM), most of the penetrated oocytes formed one or more pronuclei and an extruded second polar body. In oocytes with or without BAPTA/AM treatments, cdc2 kinase activity decreased to the basal level at 8 h after onset of developmental culture. The MAP kinase activities were significantly reduced at 8 h after onset of developmental culture compared to that before in vitro fertilization. Therefore, we propose that the activation of bovine oocytes at fertilization may be directly induced by as yet unknown sperm-derived activator(s) that is(are) different from the factor inducing oscillation of calcium in spermatozoa.

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“…Thus, olive oil generated the greatest number of sperm per oocyte as well as the higher rate of penetration. Penetration testing conducted by co-incubation of gametes is more predictive of fertility compared to tests that evaluate the sperm alone 41,42 , in addition to this test have shown sensitivity in detecting fertilizing ability of boar sperm 29 .…”

Section: Discussionmentioning

confidence: 99%

“…After co-incubation, oocytes were recovered, washed, stained with Hoechst 33342 (10 mg / ml) and evaluated in epifluorescence microscope at 400x. Finally, oocytes were considered penetrated when their zona pellucida contained at least one sperm in perivitelline or cytoplasm space 29,30 .…”

Section: Methodsmentioning

confidence: 99%

Olive oil as an alternative to boar semen cryopreservation

Silva

Cardoso

Dutra³

et al. 2016

Ces. Med. Vet. Zootec.

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Activation of hamster zona-free oocytes by homologous and heterologous spermatozoa

Abstract: Spermatozoa of a wide variety of species can fuse with zona-free hamster oocytes. Zona

Cited by 37 publications

References 1 publication

Male accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters

Male accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters

Activation by Stimuli of Sperm Penetration in Bovine Oocytes Treated with Higher Concentration of BAPTA/AM.

Olive oil as an alternative to boar semen cryopreservation

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