Activation of Immune Responses in Mice by an Oral Administration of Bunching Onion (Allium fistulosum) Mucus

Ueda, Hiroshi; Takeuchi, Atsuko; Wako, Tadayuki

doi:10.1271/bbb.130084

Cited by 19 publications

(19 citation statements)

References 22 publications

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“…The green leaf mucus of Japanese bunching onion activated murine macrophages and splenic NK cells (Ueda et al, 2013). In addition, oral administration of the mucus to mice enhanced natural immunity, evidenced by the increased cytokine release and the phagocytic activity of macrophages.…”

Section: Discussionmentioning

confidence: 94%

“…Japanese bunching onion secretes mucus in its hollow tubelike green leaves. The mucus was shown to increase the production of tumor necrosis factor (TNF)-α and monocyte chemotactic protein (MCP)-1 from the RAW 264 murine macrophage cell line and of interleukin (IL)-12 from J774.1 cells; however, extracts from the green leaves and white sheaths did not (Ueda et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…It also increased interferon (IFN)-γ production from spleen cells and natural killer (NK) activity, suggesting that the mucus can enhance natural immunity (Ueda et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (Allium fistulosum) Green Leaves

Yamazaki

Ogawa

Muramoto

et al. 2016

FSTR

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Proteins contained in the green leaf mucus of Japanese bunching onion (Allium fistulosum L.) were isolated by ammonium sulfate precipitation and separated by hydrophobic interaction chromatography. The mucus proteins had an enhanced effect on tumor necrosis factor (TNF)-α production in the RAW 264 murine macrophage cell line. Protein composition was analyzed by SDS-polyacrylamide gel electrophoresis and N-terminal amino acid sequencing.The effect on RAW 264 cells was mainly attributed to mannose-binding lectin and thaumatin-like proteins, which occupied 34% of the mucus proteins. Other major proteins contained in the mucus showed homologous amino acid sequences to those of lachrymatory factor synthase, β-1,3-glucanase, 2S albumin precursor, and pathogenesis-related protein 4. Protein composition ratios in the mucus of 13 cultivars of Japanese bunching onion were also examined.Taken together, the green leaf mucus of Japanese bunching onion may be a possible source of various bioactive proteins.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (Allium fistulosum) Green Leaves

Yamazaki

Ogawa

Muramoto

et al. 2016

FSTR

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“…Peak concentrations of quercetin were observed 7.0 ± 2.9 h after ingestion, and its elimination half-life was about 11 h. Also, the plant matrix influences both the rate and extent of absorption. This plant acts as a booster of the immune system [8], and its effect on cytokines reflects its immunomodulatory property [9]. Anti-asthmatic properties of the extract of A. cepa and its constituent quercetin on cytokines, inflammatory cells, and smooth muscle contraction were shown in a murine model of asthma [10].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Allium cepa Extract on Lung Oxidant, Antioxidant, and Immunological Biomarkers in Ovalbumin-Sensitized Rats

et al. 2018

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Objectives: To evaluate the effects of Allium cepa (A. cepa) on levels of oxidants, antioxidants, and immunological markers in bronchoalveolar lavage fluids (BALF) of sensitized rats. Materials and Methods: Oxidant/antioxidant markers and cytokines in BALF of control rats treated with saline (group C), ovalbumin-sensitized rats (group S), rats treated with 1.25 μg/mL dexamethasone and 3 doses of A. cepa extract (35, 70, and 140 mg/kg body weight [BW]/day) (S + AC) were investigated. Comparison of the results between groups was performed using analysis of variance with the Tukey-Kramer post hoc test. Results: The oxidant markers nitrogen dioxide (NO₂), nitrate (NO₃^–), and malondialdehyde (MDA), and immunological markers interleukin (IL)-4 and immunoglobulin E (IgE) were significantly higher, but the antioxidant markers superoxide dismutase (SOD), catalase (CAT), thiol, and interferon (IFN)-γ, and the IFN-γ/IL-4 ratio were lower in sensitized rats compared to control rats (p < 0.001 to p < 0.01). Compared to group S, the levels of the following markers were significantly lower: NO₂, NO₃^–, and IgE in groups treated with the A. cepa extract, MDA and IL-4 levels in groups treated with 70 and 140 mg/kg BW/day of the A. cepa extract, and all these markers as well as IFN-γ in rats treated with dexamethasone (p < 0.001 to p < 0.05). However, there were significantly higher levels of SOD and CAT and an increased IFN-γ/IL-4 ratio (groups treated with 70 and 140 mg/kg BW/day of the A. cepa extract), and levels of thiol and IFN-γ (group treated with 140 mg/kg BW/day of the A. cepa extract) as well as SOD, CAT, and thiol (dexamethasone-treated group) versus group S (p < 0.00 to p < 0.05). Conclusion: A. cepa showed antioxidant and immunomodulatory properties in sensitized rats.

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“…However, Colombia's production is based on clonal propagation due to its temperate origin and vernalization requirements for bolting [8]. Currently, bunching onion research has mostly been focused on yield [8], freezing tolerance [9], resistance to Fusarium oxysporum [10,11], extraction of medicinal compounds [6,12], and pungency reduction [13].…”

Section: Introductionmentioning

confidence: 99%

Agronomic Evaluation of Bunching Onion in the Colombian Cundiboyacense High Plateau

Galeano-Mendoza

Cubillos

Varón

et al. 2018

International Journal of Agronomy

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Bunching onion (Allium fistulosum L.) is a strategic crop for Colombia due to its economic relevance within fresh and processed food markets, and therefore, there is a demand for high yielding genotypes adapted to specific regions. For this reason, after carrying out a clonal selection process including 62 genotypes, ten of these, including a regional control, were evaluated for six different traits in Boyacá (Colombia) during 2012 and 2013. These traits were grouped into agronomic, yield, and processing categories. In general, these showed significant differences ( ≤ 0.01) for genotypes, location, and genotypes × location interaction. Compared with the regional control and based on the multienvironmental analysis the genotypes Clone 30 and Clone 38 were the most promising new cultivars identified in this study. These two clones showed comparative advantages on earliness and yield, and they moreover showed some level of resistance to downy mildew and root rot, the most limiting diseases for Boyacá's bunch onion farmers. Therefore, Clone 30 and Clone 38 were registered as new bunching onion cultivars for the Cundiboyacense High Plateau region under the names Corpoica Aquitania-1 and Corpoica Tota-1, respectively. Finally, further approaches and initiatives on bunching onion breeding are discussed.

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Activation of Immune Responses in Mice by an Oral Administration of Bunching Onion (Allium fistulosum) Mucus

Cited by 19 publications

References 22 publications

Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (<i>Allium fistulosum</i>) Green Leaves

Isolation and Biochemical Characterization of Mucus Proteins in Japanese Bunching Onion (<i>Allium fistulosum</i>) Green Leaves

The Effect of <b><i>Allium cepa</i></b> Extract on Lung Oxidant, Antioxidant, and Immunological Biomarkers in Ovalbumin-Sensitized Rats

Agronomic Evaluation of Bunching Onion in the Colombian Cundiboyacense High Plateau

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