Activation of infectious SV40 synthesis in transformed cells.

Kit, Saul; Kurimura, Takashi; Salvi, M. L.; Dubbs, D. R.

doi:10.1073/pnas.60.4.1239

Cited by 50 publications

(26 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the association persisted through zonal and equilibrium sedimentation in alkali gradients, where only denatured, single-stranded DNA exists; the persistence shows that cellular and viral DNA's are held together by covalent bonds. 964 These results are strengthened by the observation that cells transformed by SV40, which, as I show below, contain a complete viral genome, do not yield infectious viral DNA on extraction (13).…”

Section: Stable and Abortive Transformation By Dna Virusesmentioning

confidence: 94%

Cell Transformation by Viruses

Dulbecco

1969

Science

120

View full text Add to dashboard Cite

Section: Stable and Abortive Transformation By Dna Virusesmentioning

confidence: 94%

Cell Transformation by Viruses

Dulbecco

1969

Science

120

View full text Add to dashboard Cite

“…Heterokaryons formed by fusion of these transformed cells with uninfected permissive monkey cells support the activation of the resident viral genome leading to subsequent viral DNA replication, late protein synthesis, maturation and release of virus (17,34). The time course of appearance of infectious virus from heterokaryon cultures and productive infection of permissive monkey cells is similar (16,34).…”

Section: Introductionmentioning

confidence: 96%

Rescue of SV 40 from interferon-treated heterokaryons

Mozes¹

1982

Archives of Virology

View full text Add to dashboard Cite

Simian virus 40 (SV40)-transformed nonpermissive cells express only the early products of SV40. Heterokaryons formed by fusion of these transformed cells with uninfected permissive cells support the activation of the resident viral genome leading to subsequent viral DNA replication, late protein synthesis and release of progeny virus. Pretreatment of heterokaryon cultures with either mouse or monkey interferon (IFN) before fusion with polyethylene glycol (PEG) produced a dose-dependent inhibition in the appearance of free viral DNA as well as production of infectious virus. The decreased yield of SV40 in these cultures was similar to the inhibition which was observed in mouse or monkey cells incubated with homologous IFN prior to exogenous infection with SV40. When IFN was added to the cultures at progressively later times after fusion with PEG, there was less inhibition of virus production. Although there was a comparable decrease in the production of virus by pretreatment with either mouse or monkey IFN, monkey IFN exerted the inhibition for a longer period of time when added after heterokaryon formation. These results demonstrate that IFN treatment applied even after initiation of SV40 replication can still inhibit virus multiplication.

show abstract

“…For each sample, 5-ml aliquots were seeded in two plastic dishes (6 cm in diameter) and in a plastic flask (25 cm2 (ME, L) * Yields of infectious SV40 DNA were comparable to those quoted in Table 1. concentration of the extract. Untreated TSV-5 C12 cells do not shed SV40 virions spontaneously (24), nor can infectious viral DNA be extracted from them (4,18). No infectious SV40 DNA was ever found in cells treated with poly(iornithine) alone, with dilutions of Tris* HCI buffer in poly(L-ornithine), or with deoxyribonuclease in poly(L-ornithine), nor in the controls with any other extract.…”

Section: Assay For Activationmentioning

confidence: 99%

Activation of the Viral Genome in Simian Virus 4-Transformed Nonpermissive Cells by Permissive Cell Extracts

Suárez

Sonenshein

Estrade

et al. 1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Simian virus 40-transformed nonpermissive cells in which neither infectious viral DNA nor virions had previously been detected reproducibly yield low levels of infectious simian virus-40 DNA after treatment with extracts of normal permissive cells. Virions were obtained in one out of 37 experiments. Activation of the viral genome is detected only with normal permissive cell extracts and only in simian virus 40-transformed cell lines that are rescuable by heterokaryon formation or by treatment with chemicals. The activating factor(s) is insensitive to deoxyribonuclease and ribonuclease, but is sensitive to heat at 560 and to proteolytic enzymes.

show abstract

Activation of infectious SV40 synthesis in transformed cells.

Cited by 50 publications

References 18 publications

Cell Transformation by Viruses

Cell Transformation by Viruses

Rescue of SV 40 from interferon-treated heterokaryons

Activation of the Viral Genome in Simian Virus 4-Transformed Nonpermissive Cells by Permissive Cell Extracts

Contact Info

Product

Resources

About