Activation of Macrophages &lt;i&gt;in vitro&lt;/i&gt; by Phospholipids from Brain of &lt;i&gt;Katsuwonus pelamis&lt;/i&gt; (Skipjack Tuna)

Lu, Hang; Zhang, Li; Li, Jingjing; You, Hongjian; Jiang, Lu; Hu, Jianen

doi:10.5650/jos.ess17181

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…In the present study, rFIP-glu significantly improved the phagocytosis of macrophage RAW264.7 cells at 2 μg/mL, suggesting that rFIP-glu have abilities to enhance phagocytic activity of RAW 264.7 cells. The phagocytosis of macrophages can be enhanced by many bioactive substances, such as polysaccharides (42, 43), peptides (44) and proteins (45, 46), alkaloids (47) and phospholipids (48). Phagocytosis is one of the important innate immune responses.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

Chang

et al. 2018

Preprint

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Ganoderma lucidum, a traditional edible and medicinal fungus, holds an important status in health care systems in China and other Asian countries. Fungal immunomodulatory protein (FIP), one of the active ingredients isolated from G. lucidum, is a class of naturally occurring proteins and possesses potential biological functions. This study was conducted to explore the molecular mechanism of its immunomodulatory potency in immune responses of macrophages. In vitro assays of biological activity indicated that rFIP-glu significantly activated macrophage RAW264.7 cells, and possessed the ability of pro- and anti-inflammation the cells. RNA sequencing analysis showed that macrophage activation involved Toll-like receptors and mitogen-activated protein kinases pathways. Furthermore, qRT-PCR indicated that phosphoinositide 3 kinase inhibitor LY294002 blocked the mRNA levels of MCP-1, MEK1/2 inhibitor U0126 reduced the mRNA levels of TNF-α and MCP-1, and JNK inhibitor SP600125 prevented the up-regulation of iNOS mRNA in the rFIP-glu-induced cells. FIP-glu mediated these inflammatory effects not through a general pathway, instead through a different pathway for different inflammatory mediator. These data indicate the possibility that rFIP-glu has an important immune-regulation function and thus has potential therapeutic uses.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

Chang

et al. 2018

Preprint

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“…Another study highlighted the anti-inflammatory activities of extracts from gloomy octopus (Octopus tetricus) viscera, squid (Sepioteuthis australis) heads, Australian sardine (Sardinops sagax) viscera/heads, salmon (S. salar) heads, and school prawn (Penaeus plebejus) viscera/heads [169]. Also, phospholipid extracts from the brain of skip-jack tuna (K. pelamis) were shown to display macrophage-activating activity by inducing pro-inflammatory cytokines, therefore being suggested as possible boosters for human immunity [231]. Lipid extracts from different organs (stomach, liver, brain, and skin) of marbled rockcod (N. rossii) and mackerel icefish (C. gunnari) were tested for their potential in skin protection, with promising results [110].…”

Section: The Value Of Marine Animal Co-product Lipids For Various Ind...mentioning

confidence: 99%

Marine Animal Co-Products—How Improving Their Use as Rich Sources of Health-Promoting Lipids Can Foster Sustainability

Monteiro,

Domingues,

Calado

2024

Marine Drugs

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Marine lipids are recognized for their-health promoting features, mainly for being the primary sources of omega-3 fatty acids, and are therefore critical for human nutrition in an age when the global supply for these nutrients is experiencing an unprecedent pressure due to an ever-increasing demand. The seafood industry originates a considerable yield of co-products worldwide that, while already explored for other purposes, remain mostly undervalued as sustainable sources of healthy lipids, often being explored for low-value oil production. These co-products are especially appealing as lipid sources since, besides the well-known nutritional upside of marine animal fat, which is particularly rich in omega-3 polyunsaturated fatty acids, they also have interesting bioactive properties, which may garner them further interest, not only as food, but also for other high-end applications. Besides the added value that these co-products may represent as valuable lipid sources, there is also the obvious ecological upside of reducing seafood industry waste. In this sense, repurposing these bioresources will contribute to a more sustainable use of marine animal food, reducing the strain on already heavily depleted seafood stocks. Therefore, untapping the potential of marine animal co-products as valuable lipid sources aligns with both health and environmental goals by guaranteeing additional sources of healthy lipids and promoting more eco-conscious practices.

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“…To investigate the viability of RAW 264.7 cells treated with PLW, the number of living cells was determined using the 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method [13][14][15][16]. The cells were then removed from the culture dish, seeded at a density of 1.5×10 5 cells/well in the medium, added to 24-well plates, incubated for 24 h, and then treated with the PLW samples.…”

Section: Measurement Of Cell Viabilitymentioning

confidence: 99%

“…The amount of nitrite accumulated in the activated macrophage culture was measured after processing the sample in the same manner as in the MTT assay [15]. After the same amount of Griess reagent was mixed in 100 µL of the cell culture solution, the absorbance at 540 nm was measured using a microplate reader, and the amount of NO produced was calculated based on the control (cells treated with only lipopolysaccharide [LPS]).…”

Section: Measurement Of No Productionmentioning

confidence: 99%

“…In this regard, immunomodulation is emerging as an important strategy for improving the immune defense system, and research is underway to advance the development of natural substances that activate macrophages. Several types of immunomodulators have been recently identified, including natural polysaccharides purified from botanical sources, marine phospholipids with omega-3 polyunsaturated fatty acids (docosahexaenoic acid and eicosapentaenoic acid), and polyphenols derived from thinned peaches [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Carica papaya leaf water extract promotes innate immune response via MAPK signaling pathways

Hyun

2021

JABC

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The emergence and rapid spread of the potentially fatal coronavirus disease 2019, caused due to infection by severe acute respiratory syndrome coronavirus-2, has led to worldwide interest in developing functional bioactive ingredients that act as immunomodulatory agents. In this study, we aimed to characterize Carica papaya extract and explore its potential as an immunomodulator by performing in vitro cell screening. Papaya leaf water extract (PLW) was found to significantly increase the levels of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) by upregulating inducible nitric oxide synthase and cyclo-oxygenase-2 activity, respectively. Additionally, PLW increased the production of tumor necrosis factor-α and interleukin 1β in RAW 264.7 cells. Furthermore, PLW activated the expression of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) but not that of p38 mitogen-activated protein kinase. These results indicate that PLW increased the production of NO, PGE 2 , and pro-inflammatory cytokines by activating the JNK and ERK pathways in macrophages, thus demonstrating immunomodulatory properties. Finally, high-performance liquid chromatography fingerprint analysis indicated the presence of rutin, narirutin, and ρ-coumaric acid in PLW (6.30, 119.76, and 47.25 ppm, respectively). Treating cells with these compounds at non-toxic concentrations had no effect on NO production. Taken together, these results suggest that PLW may have potential as an immunity-enhancing supplement.

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Activation of Macrophages <i>in vitro</i> by Phospholipids from Brain of <i>Katsuwonus pelamis</i> (Skipjack Tuna)

Cited by 6 publications

References 26 publications

Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

Marine Animal Co-Products—How Improving Their Use as Rich Sources of Health-Promoting Lipids Can Foster Sustainability

Carica papaya leaf water extract promotes innate immune response via MAPK signaling pathways

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