Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize

Xiao, Senlin; Zang, Jie; Pei, Yuanrong; Liu, Jie; Liu, Juan; Song, Wei; Shi, Zi; Su, Aiguo; Zhao, Jiuran; Chen, Huabang

doi:10.1016/j.molp.2020.07.002

Cited by 46 publications

(32 citation statements)

References 58 publications

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“…For example, many years ago, researchers discovered that an insufficient sugar supply during anther development leads to male sterility [20]. In maize cytoplasmic male sterile lines, starch biosynthesis during pollen maturity is related to changes in the expression patterns of many sugar metabolism genes [21]. This suggests that sugar biosynthesis or transporter genes are associated with pollen development in CMS plants.…”

Section: Introductionmentioning

confidence: 99%

Comparative Cytological and Transcriptome Analyses of Anther Development in Nsa Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Brassica napus Produced by Distant Hybridization

Xing

Guan²,

Guan

2022

IJMS

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1258A is a new line of B.napus with Nsa cytoplasmic male sterility (CMS) with potential applications in hybrid rapeseed breeding. Sterile cytoplasm was obtained from XinJiang Sinapis arvensis through distant hybridization and then backcrossed with 1258B for many generations. However, the characteristics and molecular mechanisms underlying pollen abortion in this sterile line are poorly understood. In this study, a cytological analysis revealed normal microsporogenesis and uninucleate pollen grain formation. Pollen abortion was due to non-programmed cell death in the tapetum and the inability of microspores to develop into mature pollen grains. Sucrose, soluble sugar, and adenosine triphosphate (ATP) contents during microspore development were lower than those of the maintainer line, along with an insufficient energy supply, reduced antioxidant enzyme activity, and substantial malondialdehyde (MDA) accumulation in the anthers. Transcriptome analysis revealed that genes involved in secondary metabolite biosynthesis, glutathione metabolism, phenylpropane biosynthesis, cyanoamino acid metabolism, starch and sucrose metabolism, and glycerolipid metabolism may contribute to pollen abortion. The down regulation of nine cytochrome P450 monooxygenases genes were closely associated with pollen abortion. These results suggest that pollen abortion in 1258A CMS stems from abnormalities in the chorioallantoic membranes, energy deficiencies, and dysfunctional antioxidant systems in the anthers. Our results provide insight into the molecular mechanism underlying pollen abortion in Nsa CMS and provide a theoretical basis for better heterosis utilization in B.napus.

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Section: Introductionmentioning

confidence: 99%

Comparative Cytological and Transcriptome Analyses of Anther Development in Nsa Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Brassica napus Produced by Distant Hybridization

Xing

Guan²,

Guan

2022

IJMS

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“…Many novel chimeric mitochondrial genes have been identified and are responsible for CMS through a variety of mechanisms (such as orf79/orfH79/WA352 from rice, urf13/orf355 from maize, pcf from petunia, orf224/orf222 from rapeseed, etc.) [3,[6][7][8][9][10][11][12][13]. The fertility restoring genes are present in the nucleus, and most of them are triangular pentapeptide repeat (PPR) proteins (such as Rf1a and Rf1b to orf79 in rice, Rf-PPR592 to pcf in petunia, etc.)…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Mitochondrial Genome of a Wheat AL-Type Male Sterility Line and the Candidate CMS Gene

Hao

Yang

et al. 2021

IJMS

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Heterosis utilization is very important in hybrid seed production. An AL-type cytoplasmic male sterile (CMS) line has been used in wheat-hybrid seed production, but its sterility mechanism has not been explored. In the present study, we sequenced and verified the candidate CMS gene in the AL-type sterile line (AL18A) and its maintainer line (AL18B). In the late uni-nucleate stage, the tapetum cells of AL18A showed delayed programmed cell death (PCD) and termination of microspore at the bi-nucleate stage. As compared to AL18B, the AL18A line produced 100% aborted pollens. The mitochondrial genomes of AL18A and AL18B were sequenced using the next generation sequencing such as Hiseq and PacBio. It was found that the mitochondrial genome of AL18A had 99% similarity with that of Triticum timopheevii, AL18B was identical to that of Triticum aestivum cv. Chinese Yumai. Based on transmembrane structure prediction, 12 orfs were selected as candidate CMS genes, including a previously suggested orf256. Only the lines harboring orf279 showed sterility in the transgenic Arabidopsis system, indicating that orf279 is the CMS gene in the AL-type wheat CMS lines. These results provide a theoretical basis and data support to further analyze the mechanism of AL-type cytoplasmic male sterility in wheat.

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“…However, the regulatory mechanism underlying the interaction of mitochondrial-nuclear genes in CMS-S in maize remains largely unclear. In a recent study, Xiao et al (2020) identified orf355 as the causal gene of maize CMS-S. They discovered that the accumulation of orf355 transcripts in micro-spores caused mitochondrial abnormality and enhanced retrograde responses at the large vacuole stage in the CMS-S maize, which could significantly enhance the expression of genes involved in mitochondrial retrograde responses.…”

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confidence: 99%

Male Sterility in Maize: A Precise Dialogue between the Mitochondria and Nucleus

Ling

2020

Molecular Plant

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Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize

Cited by 46 publications

References 58 publications

Comparative Cytological and Transcriptome Analyses of Anther Development in Nsa Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Brassica napus Produced by Distant Hybridization

Comparative Cytological and Transcriptome Analyses of Anther Development in Nsa Cytoplasmic Male Sterile (1258A) and Maintainer Lines in Brassica napus Produced by Distant Hybridization

Characterization of the Mitochondrial Genome of a Wheat AL-Type Male Sterility Line and the Candidate CMS Gene

Male Sterility in Maize: A Precise Dialogue between the Mitochondria and Nucleus

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