Activation of phospholipase D and the possible mechanism of activation in wound-induced lipid hydrolysis in castor bean leaves

Ryu, Stephen B.; Wang, Xuemin

doi:10.1016/0005-2760(96)00096-3

Cited by 133 publications

(108 citation statements)

References 21 publications

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“…Both PLD and its product PA play key roles in plant growth and development, hormone effects and stress responses (reviewed by Wang 2005; Bargmann and Munnik 2006). PLD activity in plants, has been generally related with a wide range of stress responses, such as pathogen attack (Young et al 1996), wounding (Ryu and Wang 1996), freezing tolerance (Li et al 2004), water stress (Munnik et al 2000;Thiery et al 2004) and ABA signalling ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

2008

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The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in the induction media for five hours, highly improved embryo production in both cultivars. Sec-and tert-butanol performed similarly to control plates. Regeneration ability was unaffected by any butylalcohol treatment. As a consequence of the higher embryo production after n-butanol treatment, the number of green regenerated plants increased up to 5 times in cultivar Pavon and up to 3 times in cultivar Caramba. The percentage of green plants was improved or unaffected by the treatment. Doubled haploid plant production was between 2 and 4 times higher after n-butanol treatment than in control plates. Therefore, n-butanol was successfully applied in the production of wheat doubled haploids. This primary alcohol is known as an activator of phospholipase D and has been previously reported to disrupt cortical microtubules and detach them from the plasma membrane in plants. Its effects on androgenetic induction could confirm the importance of microtubule regulation in plant cell fate, specifically in microspore development. A possible implication of phospholipase D is discussed.

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Section: Introductionmentioning

confidence: 99%

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

2008

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“…In plants, most reports on PLD functional studies have dealt with its possible effects on membrane deterioration in relation to stress injuries, senescence, aging, and pathogenesis (4 -6). Recent studies have, however, indicated a role of PLD in signal transduction processes in response to environmental cues (7)(8)(9).…”

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confidence: 99%

Identification and Characterization of a Novel Plant Phospholipase D That Requires Polyphosphoinositides and Submicromolar Calcium for Activity in Arabidopsis

Pappan

Zheng

Wang

1997

Journal of Biological Chemistry

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Phospholipase D (PLD; EC 3.1.4.4) has been proposed to be involved in a number of cellular processes including transmembrane signaling and membrane deterioration. PLD previously described from various plant sources generally requires millimolar ranges of calcium for optimal activity. In this study, we genetically suppressed the expression of this conventional PLD in Arabidopsis by introducing an antisense PLD cDNA. However, both the antisense transgenic and wild-type plants showed comparable PLD activity in the presence of submicromolar concentrations of calcium and phosphatidylinositol 4,5-bisphosphate using phosphatidylcholine as a substrate. This new PLD activity was partially stimulated by phosphatidylinositol 4-phosphate, but not by other phospholipids, including phosphatidylinositol, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, or phosphatidylcholine. Its requirement for polyphosphoinositides was further supported by its ability to be inhibited by neomycin. The polyphosphoinositide-dependent PLD requires calcium for activity, but no magnesium. The calcium stimulation was observed in the nanomolar range and reached a plateau at 5 M calcium. The findings of this study demonstrate the presence of different PLDs that are regulated in a distinct manner in plants. The potential significance of a PLD that is regulated by polyphosphoinositides and physiological concentrations of Ca 2؉ is discussed.Phospholipase D (PLD; EC 3.1.4.4) 1 hydrolyzes the terminal phosphodiester bond of phospholipids, generating phosphatidic acid (PA) and a water-soluble head group. PLD-mediated hydrolysis plays an important role in cell signaling and regulation. In mammalian systems, PLD has been proposed to be involved in a broad range of cellular processes including membrane trafficking, cell proliferation, protein secretion, metabolic regulation, and immune and inflammatory responses (reviewed in Ref.

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“…This domain is important in the Ca 2ϩ -regulated translocation of proteins to membranes. Indeed, in the wound activation of PLD in castor bean, the Ca 2ϩ -mediated translocation of PLD from the cytosol to the membranes had already been proposed before the presence of a C2 domain on PLDs was recognized (Ryu and Wang, 1996). There is also data suggesting Ca 2ϩ -mediated activation of PLD in vivo (Munnik et al, 1998).…”

Section: Regulation and Activation Of Pldmentioning

confidence: 99%

“…These increases were regarded initially as autolysis resulting from the release of PLD and other lipolytic enzymes during cell damage. Recent studies have shown that wounding a tissue triggers a rapid activation of PLD-mediated phospholipid hydrolysis not only at the wound site but also at undamaged areas (Ryu and Wang, 1996). Stimulation of plant PLD has also been shown in response to treatments with ABA, light, fungal elicitors, and bacterial pathogens (Young et al, 1996;Fan et al, 1997;Chapman, 1998;Munnik et al, 1998;Ritchie and Gilroy, 1998).…”

Section: Regulation and Activation Of Pldmentioning

confidence: 99%

“…PLD can be activated rapidly by stress injuries such as mechanical wounding, frost, and ␥-irradiation (Voisine et al, 1993;Ryu and Wang, 1996). Apparently, wound activation of PLD results from its translocation to membranes, which is mediated by an increase in cytoplasmic Ca 2ϩ upon wounding (Ryu and Wang, 1998).…”

Section: Involvement Of Pld In Signaling Pathwaysmentioning

confidence: 99%

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The Role of Phospholipase D in Signaling Cascades1

Wang

1999

Plant Physiology

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107

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Activation of phospholipase D and the possible mechanism of activation in wound-induced lipid hydrolysis in castor bean leaves

Cited by 133 publications

References 21 publications

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

Identification and Characterization of a Novel Plant Phospholipase D That Requires Polyphosphoinositides and Submicromolar Calcium for Activity in Arabidopsis

The Role of Phospholipase D in Signaling Cascades1

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