The Role of Phospholipase D in Signaling Cascades1

Wang, Xuemin

doi:10.1104/pp.120.3.645

Cited by 107 publications

(77 citation statements)

References 36 publications

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“…PLDs represent a multiple gene family that is divided into three groups according to Ca 2ϩ requirement (Wang, 1999). Ca 2ϩ -independent PLDs are unable to achieve the transphosphatidylation reaction and cannot belong to the transduction cascade analyzed here because 1-BuOH addition blocks RAB18 expression.…”

Section: Specificity Of the Pld Stimulated By Abamentioning

confidence: 99%

“…In the same species, it was demonstrated that an increase in PLC1 activity was necessary for the induction of the RD29a, KIN2, and RD22 ABAresponsive genes . In Commelina communis guard cells, Staxen et al (1999) have shown that U73122, an inhibitor of phosphatidylinositol-PLC (PI-PLC) activity, abolished ABAinduced cytosolic [Ca 2ϩ ] oscillations and stomatal closure.The involvement of phospholipase D (PLD) in ABA responses is better understood (Wang, 1999;Munnik, 2001 Article, publication date, and citation information can be found at www.plantphysiol.org/cgi …”

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confidence: 99%

“…The involvement of phospholipase D (PLD) in ABA responses is better understood (Wang, 1999;Munnik, 2001 Article, publication date, and citation information can be found at www.plantphysiol.org/cgi…”

mentioning

confidence: 99%

“…The involvement of phospholipase D (PLD) in ABA responses is better understood (Wang, 1999;Munnik, 2001). PLD hydrolyzes constitutive phospholipids to phosphatidic acids (PAs) and free alcohols.…”

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Plasmalemma Abscisic Acid Perception Leads to RAB18Expression via Phospholipase D Activation in Arabidopsis Suspension Cells

et al. 2002

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Abscisic acid (ABA) plays a key role in the control of stomatal aperture by regulating ion channel activities and water exchanges across the plasma membrane of guard cells. Changes in cytoplasmic calcium content and activation of anion and outward-rectifying K ϩ channels are among the earliest cellular responses to ABA in guard cells. In Arabidopsis suspension cells, we have demonstrated that outer plasmalemma perception of ABA triggered similar early events. Furthermore, a Ca 2ϩ influx and the activation of anion channels are part of the ABA-signaling pathway leading to the specific expression of RAB18. Here, we determine whether phospholipases are involved in ABA-induced RAB18 expression. Phospholipase C is not implicated in this ABA pathway. Using a transphosphatidylation reaction, we show that ABA plasmalemma perception results in a transient stimulation of phospholipase D (PLD) activity, which is necessary for RAB18 expression. Further experiments showed that PLD activation was unlikely to be regulated by heterotrimeric G proteins. We also observed that ABA-dependent stimulation of PLD was necessary for the activation of plasma anion current. However, when ABA activation of plasma anion channels was inhibited, the ABA-dependent activation of PLD was unchanged. Thus, we conclude that in Arabidopsis suspension cells, ABA stimulation of PLD acts upstream from anion channels in the transduction pathway leading to RAB18 expression.Abscisic acid (ABA) regulates seed maturation and germination, adaptation of plants to water shortage, cold, and high salinity (Leung and Giraudat, 1998). Several ABA transduction mutants have been isolated in Arabidopsis in which diverse loci affecting ABA response have been identified (for review, see Merlot and Giraudat, 1997;Leung and Giraudat, 1998). Among the best characterized are the ABAinsensitive abi-1 and abi-2 mutations, which affect protein phosphatases (Leung et al., 1997); the abi-3 (Giraudat et al., 1992;Parcy et al., 1994), abi-4 (Finkelstein et al., 1998), and abi-5 (Finkelstein and Lynch, 2000), which are mutated in transcription processes; and the ABA-hypersensitive era1 mutant, deleted for the ␤-subunit of a farnesyl transferase which acts as a negative regulator (Cutler et al., 1996). In guard cells, ABA transduction pathways have been extensively analyzed. Stomatal aperture is controlled by ABA through the activation of anion currents, which depolarizes the plasma membrane and promotes the activation of outward-rectifying K ϩ currents (Schroeder et al., 2001). In aleurone cells, ABA causes a decrease in cytoplasmic Ca 2ϩ content, which is necessary for the inhibition of GA promotion of ␣-amylase activity (Ritchie and Gilroy, 1998). In Arabidopsis suspension cells, similar ion channel activation has been observed (Jeannette et al., 1999; Ghelis et al., 2000a Ghelis et al., , 2000bTakahashi et al., 2001). However, the precise sequence of events triggered by ABA in these models remains unknown.Recent research has provided new data about the role of phospholipases...

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Section: Specificity Of the Pld Stimulated By Abamentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Plasmalemma Abscisic Acid Perception Leads to RAB18Expression via Phospholipase D Activation in Arabidopsis Suspension Cells

et al. 2002

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“…Multiple PLD enzymes have been purified from plant cells and tissues (Dyer et al, 1994;Wissing et al, 1996), and several cDNAs and genes encoding PLD have been cloned from different plant species. These PLD-encoding genes and their products seem to be differentially regulated via transcriptional and post-translational mechanisms (reviewed in Wang, 1999). A rapid and transient increase in the levels of LA and lysophospholipids (Conconi et al, 1996;Lee et al, 1997) also has been demonstrated in wounded tomato leaves, but the nature of the hydrolytic enzymes, that is, phospholipase A 1 (PLA 1 ), PLA 2 , or PLB , was not established.…”

Section: Introductionmentioning

confidence: 99%

Positional Specificity of a Phospholipase A Activity Induced by Wounding, Systemin, and Oligosaccharide Elicitors in Tomato Leaves

Narváez-Vásquez¹,

Florin‐Christensen²,

Ryan³

1999

The Plant Cell

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Phospholipase A (PLA) activity, as measured by the accumulation of 14 C-lysophosphatidylcholine in leaves of tomato plants, increased rapidly and systemically in response to wounding. The increase in PLA activity in the systemic unwounded leaves was biphasic in wild-type tomato plants, peaking at 15 min and again at 60 min, but the second peak of activity was absent in transgenic prosystemin antisense plants. Supplying young excised tomato plants with the polypeptide hormone systemin also caused 14 C-lysophosphatidylcholine to increase to levels similar to those induced by wounding, but the increase in activity persisted for Ͼ 2 hr. Antagonists of systemin blocked both the release of 14 Clysophosphatidylcholine and the accumulation of defense proteins in response to systemin. 14 C-lysophosphatidylcholine levels did not increase in response to jasmonic acid. Chemical acylation of the lysophosphatidylcholine produced by wounding, systemin, and oligosaccharide elicitors followed by enzymatic hydrolysis with lipases of known specificities demostrated that the lysophosphatidylcholine is generated by a PLA with specificity for the sn-2 position.

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Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

Coraggio

Tuberosa

2004

Handbook of Plant Biotechnology

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Abiotic stress has become the greatest threat to global food production and security, particularly in developing countries. Progress in increasing yield and its stability under adverse conditions through a direct selection has been hampered by the low heritability of yield, especially under drought. A better knowledge of the molecular and genetic basis of the mechanisms promoting tolerance to abiotic stress will enhance our capacity to more appropriately manipulate the genes limiting crop yield under hostile environments. This review describes and critically analyses the major advances in our understanding of the complex mechanisms underlying the adaptive response of the plant to abiotic stress, starting with the perception of the stress at the cellular level, its transduction through a cascade of signalling molecules and ending with the induction of genes whose products mitigate the harmful effects of the stress. Molecular markers and the ‘omics’ platforms (e.g., transcriptomics, proteomics, metabolomics etc.) offer unprecedented opportunities to identify and clone the genes governing plant adaptation to environmental stress and limiting yield under such conditions. The dissection of the genetic basis of quantitative traits into their single components, the so‐called QTLs (quantitative trait loci), allows us to deploy marker‐assisted selection (MAS) for enhancing crop performance under adverse conditions. Additional opportunities are also offered by genetic engineering, particularly through the expression of transcription factors regulating a suite of genes whose product is crucial to mitigate the effects of stress. Although the applicative results so far achieved with non‐conventional approaches have been limited, their integration with conventional breeding methodologies and other interdisciplinary approaches will greatly enhance our capacity to more effectively tailor crop genome to withstand harsh environmental conditions and to improve their sustainability under marginal conditions.

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The Role of Phospholipase D in Signaling Cascades1

Cited by 107 publications

References 36 publications

Plasmalemma Abscisic Acid Perception Leads to RAB18Expression via Phospholipase D Activation in Arabidopsis Suspension Cells

Plasmalemma Abscisic Acid Perception Leads to RAB18Expression via Phospholipase D Activation in Arabidopsis Suspension Cells

Positional Specificity of a Phospholipase A Activity Induced by Wounding, Systemin, and Oligosaccharide Elicitors in Tomato Leaves

Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

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