Activation of platelets adhered on amorphous hydrogenated carbon (a-C:H) films synthesized by plasma immersion ion implantation-deposition (PIII-D)

Yang, Ping; Huang, Nan; Leng, Y.X.; Chen, Junying; Fu, Ricky K.Y.; Kwok, Sunny; Leng, Yang; Chu, Paul K.

doi:10.1016/s0142-9612(03)00091-7

Cited by 153 publications

(75 citation statements)

References 35 publications

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“…It is fully accepted that the presence of proteins adsorbed on the artificial surface play a crucial role in mediating the initial interactions of platelets with the surface, and the composition of the synthetic surface is a key determinant in the rate and nature of the protein adsorbed. 30 Our previous studies have shown that human albumin is adsorbed more easily on floating carbon thin films with nanoroughness and a low hydrogen content in plasma. In contrast, fibrinogen is favorably adsorbed into biased carbon coatings in comparison to floating ones.…”

Section: Nanomaterials Properties Dictate Platelet Responsementioning

confidence: 99%

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Karagkiozaki¹,

Karagiannidis²,

Kalfagiannis³

et al. 2012

IJN

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Background: Nanomedicine has the potential to revolutionize medicine and help clinicians to treat cardiovascular disease through the improvement of stents. Advanced nanomaterials and tools for monitoring cell-material interactions will aid in inhibiting stent thrombosis. Although titanium boron nitride (TiBN), titanium diboride, and carbon nanotube (CNT) thin films are emerging materials in the biomaterial field, the effect of their surface properties on platelet adhesion is relatively unexplored. Objective and methods: In this study, novel nanomaterials made of amorphous carbon, CNTs, titanium diboride, and TiBN were grown by vacuum deposition techniques to assess their role as potential stent coatings. Platelet response towards the nanostructured surfaces of the samples was analyzed in line with their physicochemical properties. As the stent skeleton is formed mainly of stainless steel, this material was used as reference material. Platelet adhesion studies were carried out by atomic force microscopy and scanning electron microscopy observations. A cell viability study was performed to assess the cytocompatibility of all thin film groups for 24 hours with a standard immortalized cell line. Results: The nanotopographic features of material surface, stoichiometry, and wetting properties were found to be significant factors in dictating platelet behavior and cell viability. The TiBN films with higher nitrogen contents were less thrombogenic compared with the biased carbon films and control. The carbon hybridization in carbon films and hydrophilicity, which were strongly dependent on the deposition process and its parameters, affected the thrombogenicity potential. The hydrophobic CNT materials with high nanoroughness exhibited less hemocompatibility in comparison with the other classes of materials. All the thin film groups exhibited good cytocompatibility, with the surface roughness and surface free energy influencing the viability of cells.

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Section: Nanomaterials Properties Dictate Platelet Responsementioning

confidence: 99%

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Karagkiozaki¹,

Karagiannidis²,

Kalfagiannis³

et al. 2012

IJN

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show abstract

“…The substitution of nitrogen for carbon induces a variety of new bonding configurations because nitrogen has five valence electrons, although despite this extra valence electron, nitrogen forms similar hybridized states to carbon. In sp 3 hybridization, the extra valence electron fills one of the four sp 3 orbitals, resulting in three sp 3 orbitals with one electron each, which can be used to form a trigonal pyramid, and the other sp 3 orbital is nonbonding, containing two electrons in a lone pair [2729]. The lone pair of electrons prevented charge transfer from fibrinogen to the material surface, which improved the hemocompatibility of the CN x coatings.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

“…This variability is determined by the ratio of diamond bonds (sp 3 ) to graphitic bonds (sp 2 ) in the sample [2]. The biocompatibility and low wettability of diamond-like carbon (DLC) [3] means that it shows potential for application in biology and medicine as a protective coating for implants, biosensors and biochips [2,47]. It is anticipated that amorphous DLC will be used in medical devices such as hip prostheses, coronary stents, heart valves, vascular prostheses, intraocular and contact lenses, surgical needles for corneal surgery, orthopedic pins and screws, dental prostheses, medical guidewires, and rotary pumps for left ventricular assistance [715].…”

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confidence: 99%

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In vitro comparison of the hemocompatibility of diamond-like carbon and carbon nitride coatings with different atomic percentages of N

Zhao

Zhang

et al. 2012

Sci. China Life Sci.

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Carbon nitride (CN x ) and diamond-like carbon (DLC) coatings were prepared by dc magnetron sputtering at room temperature. Different partial pressures of N 2 were used to synthesize CN x to evaluate the relationship between the atomic percentage of nitrogen and hemocompatibility. Auger electron spectroscopy and atomic force microscopy indicated atomic percentages of N of 0.12 and 0.22 and that the CN x coatings were smooth. An in vitro study of the hemocompatibility of the coatings revealed that both CN x coatings had better anticoagulant properties and lower platelet adhesion than DLC.

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“…A common failure of a biomaterial arises from thrombogenesis, which is initiated by platelets' adhesion onto artifi cial surface and activation. Thus, a signifi cant factor that determines hemocompatibility, is the platelets' response to the surface of a biomaterial, as indicated by their morphological changes (Yang 2003;Plant et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications

Karagkiozaki

Logothetidis

Lousinian³

et al. 2008

IJN

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Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin fi lms (a-C:H) was carried out by means of electrostatic force microscope (EFM) and observation of differences in spatial charge distribution on the surface of the examined fi lms during platelets adhesion was made. The thrombogenic potential of a-C:H thin fi lms developed by magnetron sputtering with ∼42% sp 3 content and hydrogen partial pressure during deposition was evaluated,by in situ observation with atomic force microscope (AFM) of platelets' activation and their subsequent adhesion. Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied. Platelets behavior and their correlation with the electric surface properties of the examined a-C:H fi lms by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus. The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon fi lms on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.

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Activation of platelets adhered on amorphous hydrogenated carbon (a-C:H) films synthesized by plasma immersion ion implantation-deposition (PIII-D)

Cited by 153 publications

References 35 publications

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Novel nanostructured biomaterials: implications for coronary stent thrombosis

In vitro comparison of the hemocompatibility of diamond-like carbon and carbon nitride coatings with different atomic percentages of N

Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications

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