Activation of RAW 264.7 cells by a polysaccharide isolated from Antarctic bacterium Pseudoaltermonas sp. S-5

Li, Jing; Wen, Qian; Xu, Yanghui; Chen, Guochuang; Wang, Guodong; Nie, Shuai; Shen, Bingxiang; Zhao, Zhigang; Liu, Chunyan; Chen, Kaoshan

doi:10.1016/j.carbpol.2015.04.070

Cited by 41 publications

(23 citation statements)

References 30 publications

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“…The morphology of macrophages changes with the cell activity, function, and environment. When stimulated with LPS or other signaling factors, the morphology of RAW264.7 cells was altered [43]. In this study, morphological changes of RAW264.7 cells treated with NJP or LPS were investigated under an inverted microscope.…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Zhang

Lin

et al. 2019

Marine Drugs

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In this study, a low molecular-weight (Mw) peptide named NJP (<1 kDa), was purified from a protein hydrolysate of Nibea japonica by ultrafiltration, and its immunomodulatory effect on RAW264.7 cells was evaluated. The lactate dehydrogenase (LDH) and MTT assays were performed to explore the cytotoxicity of NJP. The results showed that NJP promoted cell proliferation and had no significant toxic effects on RAW264.7 cells. Moreover, the cells formed multiple pseudopodia indicating that they were in activated state. Further tests showed that NJP significantly promoted phagocytic capacity, and the secretion of proinflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). It also increased the synthesis of nitric oxide (NO) by upregulating inducible nitric oxide synthase (iNOS) protein level. Flow cytometry revealed that NJP promoted cell cycle progression and increased the percentage of cells in G0/G1 phase. NJP promoted IκBα degradation, p65 and nuclear factor (NF)-κB activation and translocation by up-regulating IKKα/β protein expression. In conclusion, these results indicated that NJP exerts immunomodulatory effects on RAW264.7 cells through the NF-κB signaling pathway. Therefore, NJP can be incorporated in the production of functional foods or nutraceuticals.

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Section: Resultsmentioning

confidence: 99%

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Zhang

Lin

et al. 2019

Marine Drugs

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“…TNFa and IL-6 transcription have been documented to be inuenced by both MAPK and NF-kB pathway. 22 The transcription factor NF-kB plays a central role in the regulation of many immune and inammatory responses. 23 NF-kB forms an inactive cytoplasmic complex together with its inhibitor (IkBa) in unstimulated cells.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides fromPolygonatum sibiricumDelar. ex Redoute induce an immune response in the RAW264.7 cell lineviaan NF-κB/MAPK pathway

et al. 2019

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“…After removing the cell supernatants, an MTT assay was used to determine cell viability (Li et al, 2015;Zhang et al, 2016). After removing the cell supernatants, an MTT assay was used to determine cell viability (Li et al, 2015;Zhang et al, 2016).…”

Section: Cell Culturementioning

confidence: 99%

“…Cell viability assay RAW 264.7 cells (1 9 10 6 cells mL À1 ) were incubated for 24 h and treated with 62.5, 125, 250, 500 and 1000 lg mL À1 DAP for another 24 h. An equal volume of medium was used as controls. After removing the cell supernatants, an MTT assay was used to determine cell viability (Li et al, 2015;Zhang et al, 2016).…”

Section: Cell Culturementioning

confidence: 99%

Structural characterisation and immunomodulatory effects of polysaccharides isolated from Dendrobium aphyllum

Liu

Gong

et al. 2017

Int J of Food Sci Tech

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Summary A crude polysaccharide extract of Dendrobium aphyllum (cDAP, yield 38.15 ± 0.20%) was generated. The D. aphyllum polysaccharide (DAP, Mw 471.586 kDa), purified by DEAE‐Sepharose and Sephadex‐G200 Fast Flow, was composed of mannose (71.3%) and glucose (28.7%), according to GC–MS analysis. Its backbone was composed of β‐d‐mannopyranose and β‐d‐glucopyranose residues, as revealed by infrared spectroscopic analysis. Its glycosidic bond was mainly 1, 4‐linked, and the O‐acetyl groups were mainly linked to mannose residues, according to periodate oxidation and Smith degradation analysis. The DAP units polymerised into a filiform‐shaped spatial pattern, as characterised by atomic force microscopy and scanning electron microscopy. DAP treatment enhanced cytokine secretion (nitric oxide, interleukin‐6 and tumour necrosis factor‐α) and pinocytic and phagocytic capacities of RAW 264.7 mouse macrophages. The complement receptor 3 and mannose receptor were identified to be the receptors of DAP on RAW 264.7 cells, indicating that the Akt/mTOR/MAPK and IKK/nuclear factor‐ĸB pathways could be involved in DAP‐activated immunomodulation.

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Activation of RAW 264.7 cells by a polysaccharide isolated from Antarctic bacterium Pseudoaltermonas sp. S-5

Cited by 41 publications

References 30 publications

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Polysaccharides fromPolygonatum sibiricumDelar. ex Redoute induce an immune response in the RAW264.7 cell lineviaan NF-κB/MAPK pathway

Structural characterisation and immunomodulatory effects of polysaccharides isolated from Dendrobium aphyllum

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