Polysaccharides from<i>Polygonatum sibiricum</i>Delar. ex Redoute induce an immune response in the RAW264.7 cell line<i>via</i>an NF-κB/MAPK pathway

Zhang, Jiaozhen; Liu, Na; Sun, Chao; Sun, Deqing; Wang, Yongjie

doi:10.1039/c9ra03023a

Cited by 37 publications

(14 citation statements)

References 27 publications

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“…NF-κB plays a key role in the regulation of immune and inflammatory responses through phosphorylation, DNA binding, dimerization and nuclear translocation methods (39,40). p65 is key in the activation of the NF-κB family of transcription factors and translocates from the cytoplasm to the nucleus after stimulation (41,42). In the present study, p65 nuclear translocation increased in RAW264.7 cells treated with APS as determined by EMSA.…”

Section: Aps (µG/ml) ------------------------------------------------supporting

confidence: 55%

Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑κB p65/MAPK signaling pathway

et al. 2020

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The aim of the present study was to investigate the immunoregulatory effects of Astragalus polysaccharide (APS) on RAW264.7 cells. The production of cytokines by RAW264.7 cells was analyzed using ELISA, while cell viability and optimal concentration of APS were assessed using the Cell Counting Kit-8 assay. In addition, the mRNA levels of IL-6, inducible nitric oxide synthase (iNOS) and TNF-α were determined by reverse transcription-quantitative PCR analysis. The levels of co-stimulatory molecules and cell cycle distribution were assessed by flow cytometry. Electrophoretic mobility shift assay was used to determine the effects of APS on p65 expression. Compared with controls, APS enhanced the production of NO, the gene expression of TNF-α, IL-6 and iNOS and the protein levels of phosphorylated p65, p38, Jun N-terminal kinase and extracellular signal regulated kinase in RAW264.7 cells, whereas these effects of APS were alleviated by pyrrolidine dithiocarbamate. The results of the present study indicated that the immunoregulatory effects of APS are mediated, at least in part, via the activation of the NF-κB p65/MAPK signaling pathway.

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Section: Aps (µG/ml) ------------------------------------------------supporting

confidence: 55%

Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑κB p65/MAPK signaling pathway

et al. 2020

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“…Particularly, M1-polarized macrophages could mediate host defense against the microbial infections and tumors, while M2 macrophages, featuring CD206, IL-10, Arg-1, and TGF-β, were involved in immune tolerance and tumor progression [ 58 ]. Furthermore, PSP-induced dendritic-like morphological changes caused IkB-α degradation, prompted NF-kB p65 translocation into the nucleus, and increased the production of immune-associated factors including NO, TNF-α, inducible nitric oxide synthase (iNOS), COX-2, NF-kB, phosphorylated p38 MAPK, and IL-6 [ 102 ]. PCP-1 exhibited immune-stimulating activity on cell viability and IL-6 production in RAW 264.7 macrophages [ 28 ].…”

Section: Applications For Health-promoting Activitiesmentioning

confidence: 99%

Health-Promoting Activities and Associated Mechanisms of Polygonati Rhizoma Polysaccharides

Wang

et al. 2023

Molecules

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Polygonati Rhizoma, a typical homology of medicine and food, possesses remarkable anti-fatigue, anti-aging, metabolic regulatory, immunomodulatory, anti-inflammatory, neuroprotective, anti-diabetes, and anti-cancer effects. Among bioactive phytochemicals in Polygonati Rhizoma, polysaccharides play important roles in the health-promoting activities through the mechanisms mentioned above and potential synergistic effects with other bioactives. In this review, we briefly introduce the updated biosynthesis of polysaccharides, the purification method, the structure characterization, and food applications, and discuss in detail the biological activities of Polygonati Rhizoma polysaccharides and associated mechanisms, aiming at broadening the usage of Polygonati Rhizoma as functional food and medicine.

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“…The vitality of macrophages is a measure of immune activation and activator cytotoxicity [ 78 ]. In a dose-dependent way, PSP caused dendritic-like morphological alterations in RAW 264.7 cells and enhanced the production of nitric oxide, TNF-α, and IL-6.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

A Review of Polygonatum Mill. Genus: Its Taxonomy, Chemical Constituents, and Pharmacological Effect Due to Processing Changes

et al. 2022

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Ethnopharmacological relevance: The genus Polygonatum Tourn, ex Mill. contains numerous chemical components, such as steroidal saponins, polysaccharides, flavonoids, alkaloids, and others, it possesses diverse pharmacological activities, such as anti-aging, anti-tumor, immunological regulation, as well as blood glucose management and fat reducing properties. Aim of the review: This study reviews the current state of research on the systematic categorization, chemical composition, pharmacological effects, and processing changes of the plants belonging to the genus Polygonatum, to provide a theoretical foundation for their scientific development and rational application. Materials and methods: The information was obtained by searching the scientific literature published between 1977 and 2022 on online databases (including PubMed, CNKI, SciFinder, and Web of Science) and other sources (such as the Chinese Pharmacopoeia 2020 edition, and Chinese herbal books). Results: The genus Polygonatum contains 79 species, and 233 bioactive chemical compounds were identified in them. The abundance of pharmacological activities, such as antioxidant activities, anti-fatigue activities, anti-inflammatory activities, etc., were revealed for the representatives of this genus. In addition, there are numerous processing methods, and many chemical constituents and pharmacological activities change after the unappropriated processing. Conclusions: This review summarizes the taxonomy classification, chemical composition, pharmacological effects, and processing of the plants belonging to the genus Polygonatum, providing references and research tendencies for plant-based drug development and further clinical applications.

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Polysaccharides fromPolygonatum sibiricumDelar. ex Redoute induce an immune response in the RAW264.7 cell lineviaan NF-κB/MAPK pathway

Abstract: Polysaccharides induce an immune response via an NF-κB/MAPK pathway.

Cited by 37 publications

References 27 publications

Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑κB p65/MAPK signaling pathway

Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF‑κB p65/MAPK signaling pathway

Health-Promoting Activities and Associated Mechanisms of Polygonati Rhizoma Polysaccharides

A Review of Polygonatum Mill. Genus: Its Taxonomy, Chemical Constituents, and Pharmacological Effect Due to Processing Changes

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