Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti‐inflammatory medication

Mackey, Abigail L.; Rasmussen, Lotte K; Kadi, Fawzi; Schjerling, Peter; Helmark, I. C.; Ponsot, Elodie; Aagaard, Per; Durigan, João Luíz Quagliotti; Kjær, Michael

doi:10.1096/fj.201500198r

Cited by 81 publications

(91 citation statements)

References 35 publications

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“…Neonatal myosin heavy chain is considered a classic marker of adult muscle regeneration . Previous studies investigating the prevalence of MHC n in vastus lateralis muscle from young untrained participants have found either no evidence of such fibers or only very few fibers . Among our group of healthy, older individuals, there was a median (range) of 1.17% (0.28%–13.33%) MHC n + fibers, which, although relatively low, reflects a substantial number of such fibers.…”

Section: Discussionmentioning

confidence: 49%

“…This raises the question regarding the significance of MHC n + fibers in uninjured healthy skeletal muscle. Induction of muscle injury leads to reexpression of MHC e/n in both rodent and human muscle, although it should be noted that several of the methods used in earlier studies to induce muscle injury are severe, leading to necrosis and requiring de novo formation of muscle fibers through the process of adult regenerative myogenesis . As such, it is highly unlikely that the MHC n + fibers we detected in the basal state in our cohort of older individuals resulted from this type of muscle regeneration.…”

Section: Discussionmentioning

confidence: 78%

“…Embryonic and neonatal MHC (MHC e/n ) are gradually replaced by adult MHC, and, at birth, only a minor proportion of muscle fibers express MHC e/n . Most reports describe a complete absence, or very low levels (<0.5%), of MHC e/n + fibers in muscle tissue from young human adults, with only a few known exceptions . Muscle injury in adult animals and humans also leads to a process of regeneration in which MHC e/n become transiently reexpressed .…”

Section: Introductionmentioning

confidence: 99%

“…Most reports describe a complete absence, or very low levels (<0.5%), of MHC e/n + fibers in muscle tissue from young human adults, with only a few known exceptions . Muscle injury in adult animals and humans also leads to a process of regeneration in which MHC e/n become transiently reexpressed . Because such fibers are rarely observed in young individuals, it can be assumed that these immature myosins are gradually removed in the following months.…”

Section: Introductionmentioning

confidence: 99%

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Molecular indicators of denervation in aging human skeletal muscle

et al. 2019

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Introduction Muscle fiber denervation increases with age, yet studies at the tissue level are sparse due to the challenging nature of establishing the relative role of regeneration and denervation. Methods Muscle biopsies were obtained from the vastus lateralis of 70 healthy men (aged 72 ± 6 years; range, 65–94). Messenger RNA (mRNA) levels of acetylcholine receptors (AchR) were measured, and sections were stained for embryonic myosin, neonatal myosin (MHCn), and neural cell adhesion molecule (NCAM). Results Embryonic myosin+ fibers were rare, while MHCn+ and NCAM+ fibers were observed in all samples. Age (range, 65–94 years) was negatively associated with AchRγ mRNA. Discussion Muscle from healthy older individuals expressed developmental myosins to varying degrees but more than has been previously reported for young individuals. Along with the AchR correlations, we propose that these findings support the presence of neuromuscular junction destabilization, denervation, and reinnervation in aging human skeletal muscle.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular indicators of denervation in aging human skeletal muscle

et al. 2019

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“…Subjects were told to refrain from exercising throughout the duration of the study, and refrain from the use of NSAIDs (Mackey et al . ).…”

Section: Methodsmentioning

confidence: 97%

The influence of capillarization on satellite cell pool expansion and activation following exercise‐induced muscle damage in healthy young men

Nederveen

Joanisse

Snijders

et al. 2018

The Journal of Physiology

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Factors that determine the skeletal muscle satellite cell (SC) response remain incompletely understood. It is known, however, that SC activation status is closely related to the anatomical relationship between SCs and muscle capillaries. We investigated the impact of muscle fibre capillarization on the expansion and activation status of SCs following a muscle-damaging exercise protocol in healthy young men. Twenty-nine young men (21 ± 0.5 years) performed 300 unilateral eccentric contractions (180 deg s ) of the knee extensors. Percutaneous muscle biopsies from the vastus lateralis and blood samples from the antecubital vein were taken prior to (Pre) exercise and at 6, 24, 72 and 96 h of post-exercise recovery. A comparison was made between subjects who had a relative low mixed muscle capillary-to-fibre perimeter exchange index (CFPE; Low group) and high mixed muscle CFPE index (High group) at baseline. Type I and type II muscle fibre size, myonuclear content, capillarization, and SC response were determined via immunohistochemistry. Overall, there was a significant correlation (r = 0.39; P < 0.05) between the expansion of SC content (change in total Pax7 cells/100 myofibres) 24 h following eccentric exercise and mixed muscle CFPE index. There was a greater increase in activated SCs (MyoD /Pax7 cells) in the High as compared to the Low CFPE group 72 h following eccentric exercise (P < 0.05). The current study provides further evidence that muscle fibre capillarization may play an important role in the activation and expansion of the SC pool during the process of skeletal muscle repair.

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Exposure to radial extracorporeal shockwaves induces muscle regeneration after muscle injury in a surgical rat model

Langendorf

Klein

Drees

et al. 2019

Journal Orthopaedic Research

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The leading cause of training interruption in sport is a muscle injury, for which the standard treatment is nonsteroidal anti‐inflammatory drugs (NSAIDs). To find alternative treatments, we investigated whether the radial extracorporeal shockwave application (rESWT) could stimulate muscle regeneration. A lesion with complete rupture (grade III muscle tear) was set in the musculus rectus femoris of 12‐week‐old Wistar rats, and the NSAID diclofenac, rESWT, or a combined therapy were applied on day 0, 3, and 5 directly following the surgery. Rats were euthanized at 2, 4, and 7 days after surgery and the area of muscle lesion was excised for histological and gene expression analysis to determine the progress in the healing of damaged fibers and tissue regeneration. The best effect on muscle regeneration was observed in the group treated with rESWT alone. Monotherapy by diclofenac showed a smaller but still positive effect and lowest effects were detected when both therapies were applied. rESWT alone demonstrated a significant upregulation of the muscle markers MyoD and myosin. The presence of myosin gene expression indicated newly formed muscle fibers, which was confirmed by hematoxylin and eosin staining. Seven days after injury the amount of mononucleated cell decreased and regenerating fibers could be detected. This effect is most pronounced in the group treated with rESWT alone. In our study, shockwaves demonstrated the best effect on muscle regeneration. Therefore, we recommend prospective clinical studies to analyze the effect of rESWT after sports trauma to improve muscle regeneration and to shorten the rehabilitation.

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Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti‐inflammatory medication

Cited by 81 publications

References 35 publications

Molecular indicators of denervation in aging human skeletal muscle

Molecular indicators of denervation in aging human skeletal muscle

The influence of capillarization on satellite cell pool expansion and activation following exercise‐induced muscle damage in healthy young men

Exposure to radial extracorporeal shockwaves induces muscle regeneration after muscle injury in a surgical rat model

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