2021
DOI: 10.3389/fcell.2021.617549
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Activation of TC10-Like Transcription by Lysine Demethylase KDM4B in Colorectal Cancer Cells

Abstract: Malignant colorectal cancers (CRCs) are characterized by enhanced migration and invasion thus acquiring the ability to metastasize. We have previously shown that the small GTPase TC10-like (TCL) contributes to aggressive migration and invasion in malignant CRC cells. TCL expression is differentially expressed in CRC cells and can be upregulated by hypoxia although the underlying epigenetic mechanism is not fully appreciated. Here, we report that differential TCL expression in CRC cells appeared to be associate… Show more

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Cited by 15 publications
(11 citation statements)
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“…Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer (50 mM Tris pH7.4, 150 mM NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche) as previously described ( Chen et al, 2020a , b , c ; Wu X. et al, 2020 ; Yang et al, 2020b ; Zhang et al, 2020 ; Chen B. et al, 2021 ; Dong et al, 2021 ). Nuclear proteins were extracted using the NE-PER Kit (Pierce) following manufacturer’s recommendation.…”
Section: Methodsmentioning
confidence: 99%
“…Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer (50 mM Tris pH7.4, 150 mM NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche) as previously described ( Chen et al, 2020a , b , c ; Wu X. et al, 2020 ; Yang et al, 2020b ; Zhang et al, 2020 ; Chen B. et al, 2021 ; Dong et al, 2021 ). Nuclear proteins were extracted using the NE-PER Kit (Pierce) following manufacturer’s recommendation.…”
Section: Methodsmentioning
confidence: 99%
“…Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer (50 mM Tris pH7.4, 150 mM NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche, Basel, Switzerland) as previously described ( Chen et al,2020a,b , c , 2021 ; Dong et al, 2020 , 2021 ; Fan et al, 2020 ; Li N. et al, 2020 ; Li et al,2020a,b ; Lv et al, 2020 ; Mao et al, 2020 ; Sun et al, 2020 ; Wu T. et al, 2020 ; Wu X. et al, 2020 ; Yang et al, 2020 ; Hong et al, 2021 ; Kong et al, 2021 ; Zhang et al, 2021 ). Specific antibodies were added to and incubated with cell lysates overnight before being absorbed by Protein A/G-plus Agarose beads (Santa Cruz, Santa Cruz, CA, United States).…”
Section: Methodsmentioning
confidence: 99%
“…Small interfering RNAs were purchased from Dharmacon. Transient transfections were performed with Lipofectamine 2000 as previously described ( Chen et al, 2021 ; Kong et al, 2021b ; Liu et al, 2021a , b ; Zhang et al, 2021 ).…”
Section: Methodsmentioning
confidence: 99%