“…Whole cell lysates were obtained by re-suspending cell pellets in RIPA buffer (50 mM Tris pH7.4, 150 mM NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche, Basel, Switzerland) as previously described ( Chen et al,2020a,b , c , 2021 ; Dong et al, 2020 , 2021 ; Fan et al, 2020 ; Li N. et al, 2020 ; Li et al,2020a,b ; Lv et al, 2020 ; Mao et al, 2020 ; Sun et al, 2020 ; Wu T. et al, 2020 ; Wu X. et al, 2020 ; Yang et al, 2020 ; Hong et al, 2021 ; Kong et al, 2021 ; Zhang et al, 2021 ). Specific antibodies were added to and incubated with cell lysates overnight before being absorbed by Protein A/G-plus Agarose beads (Santa Cruz, Santa Cruz, CA, United States).…”