Activation of the Canonical <scp>WNT</scp> Signaling Pathway Promotes Ovarian Surface Epithelial Proliferation Without Inducing β‐Catenin/Tcf‐Mediated Reporter Expression

Usongo, Macalister; Li, Xinfang; Farookhi, Riaz

doi:10.1002/dvdy.23919

Cited by 16 publications

(12 citation statements)

References 57 publications

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“…Our observation corroborates with the finding that report activity of GSK3β is required for SKOV-3 cell survival (Cao et al, 2006). Furthermore, it has been demonstrated that LiCl or Wnt3a has no effect on β-catenin/Tcf reporter activity in SKOV-3 cells (Usongo et al, 2013). In addition, we showed recently that cell viability of SKOV-3/Wnt5a was increased by 30% compared to mock.…”

Section: Substrate-dependent Cell Adhesion Assay In the Presence Of Rsupporting

confidence: 92%

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Jannesari-Ladani

Hossein²,

Izadi-Mood³

2014

Asian Pacific Journal of Cancer Prevention

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Section: Substrate-dependent Cell Adhesion Assay In the Presence Of Rsupporting

confidence: 92%

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Jannesari-Ladani

Hossein²,

Izadi-Mood³

2014

Asian Pacific Journal of Cancer Prevention

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“…β-catenin in nuclei binds to the TCF/LEF in several types of cancers for transcriptional activation of downstream genes, such as cyclinD1, c-myc and MMPs [15]–[21], which play important roles in carcinogenesis and metastasis. To explore the effect of DACT2 on Wnt signaling in human thyroid cancer, Topflash and (TCF/LEF) reporter system was employed.…”

Section: Resultsmentioning

confidence: 99%

Methylation of DACT2 Promotes Papillary Thyroid Cancer Metastasis by Activating Wnt Signaling

et al. 2014

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Thyroid cancer is the most common endocrine malignant disease and the incidence is increasing. DACT2 was found frequently methylated in human lung cancer and hepatocellular carcinoma. To explore the epigenetic change and the role of DACT2 in thyroid cancer, 7 thyroid cancer cell lines, 10 cases of non-cancerous thyroid tissue samples and 99 cases of primary thyroid cancer samples were involved in this study. DACT2 was expressed and unmethylated in K1, SW579, FTC-133, TT, W3 and 8505C cell lines. Loss of expression and complete methylation was found in TPC-1 cells. Restoration of DACT2 expression was induced by 5-aza-2′deoxycytidine treatment. It demonstrates that the expression of DACT2 was regulated by promoter region methylation. In human primary papillary thyroid cancer, 64.6% (64/99) was methylated and methylation of DACT2 was related to lymph node metastasis (p<0.01). Re-expression of DACT2 suppresses cell proliferation, invasion and migration in TPC-1 cells. The activity of TCF/LEF was inhibited by DACT2 in wild-type or mutant β-catenin cells. The activity of TCF/LEF was increased by co-transfecting DACT2 and Dvl2 in wild-type or mutant β-catenin cells. Overexpression of wild-type β-catenin promotes cell migration and invasion in DACT2 stably expressed cells. The expression of β-catenin, c-myc, cyclinD1 and MMP-9 were decreased and the level of phosphorylated β-catenin (p-β-catenin) was increased after restoration of DACT2 expression in TPC-1 cells. The expression of β-catenin, c-myc, cyclinD1 and MMP-9 were increased and the level of p-β-catenin was reduced after knockdown of DACT2 in W3 and SW579 cells. These results suggest that DACT2 suppresses human papillary thyroid cancer growth and metastasis by inhibiting Wnt signaling. In conclusion, DACT2 is frequently methylated in papillary thyroid cancer. DACT2 expression was regulated by promoter region methylation. DACT2 suppresses papillary thyroid cancer proliferation and metastasis by inhibiting Wnt signaling.

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“…Although the interaction mechanism between GAD1 and β-catenin has not yet been reported, the current data suggested that GAD1 expression controls β-catenin localization. β-catenin in nuclei binds to the TCF/LEF in several types of cancers for transcriptional activation of downstream genes, such as MMP7 , cyclinD1 , and c-myc [42-46], which play important roles in carcinogenesis and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation

et al. 2013

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BackgroundGlutamate decarboxylase 1 (GAD1), a rate-limiting enzyme in the production of γ-aminobutyric acid (GABA), is found in the GABAergic neurons of the central nervous system. Little is known about the relevance of GAD1 to oral squamous cell carcinoma (OSCC). We investigated the expression status of GAD1 and its functional mechanisms in OSCCs.MethodsWe evaluated GAD1 mRNA and protein expressions in OSCC-derived cells using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and immunoblotting analyses. To assess the critical functions of GAD1, i.e., cellular proliferation, invasiveness, and migration, OSCC-derived cells were treated with the shRNA and specific GAD1 inhibitor, 3-mercaptopropionic acid (3-MPA). GAD1 expression in 80 patients with primary OSCCs was analyzed and compared to the clinicopathological behaviors of OSCC.ResultsqRT-PCR and immunoblotting analyses detected frequent up-regulation of GAD1 in OSCC-derived cells compared to human normal oral keratinocytes. Suppression of nuclear localization of β-catenin and MMP7 secretion was observed in GAD1 knockdown and 3-MPA-treated cells. We also found low cellular invasiveness and migratory abilities in GAD1 knockdown and 3-MPA-treated cells. In the clinical samples, GAD1 expression in the primary OSCCs was significantly (P < 0.05) higher than in normal counterparts and was correlated significantly (P < 0.05) with regional lymph node metastasis.ConclusionsOur data showed that up-regulation of GAD1 was a characteristic event in OSCCs and that GAD1 was correlated with cellular invasiveness and migration by regulating β-catenin translocation and MMP7 activation. GAD1 might play an important role in controlling tumoral invasiveness and metastasis in oral cancer.

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Activation of the Canonical WNT Signaling Pathway Promotes Ovarian Surface Epithelial Proliferation Without Inducing β‐Catenin/Tcf‐Mediated Reporter Expression

Cited by 16 publications

References 57 publications

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Methylation of DACT2 Promotes Papillary Thyroid Cancer Metastasis by Activating Wnt Signaling

Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by β-catenin translocation and MMP7 activation

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