Activation of the SCPx promoter in mouse adrenocortical Y1 cells

Lopez, Dayami; Niesen, Melissa I.; Bedi, Mohini; Hale, David; McLean, Mark

doi:10.1016/j.bbrc.2007.03.194

Cited by 3 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is speculated that other 20E-induced transcription factor(s) may be involved in the AeSCP-2 gene transcription regulation. In vertebrates it was demonstrated that SCP-2/SCP-x gene expression is up-regulated by SF-1 transcription factor and cAMP (Lopez et al, 2001(Lopez et al, , 2007. Regulation by both these factors is similar to the regulatory mechanisms observed for other genes involved in steroid hormone production (Menon & Gunaga, 1974).…”

Section: Discussionmentioning

confidence: 59%

“…, 1989; Rennert et al. , 1991) and these studies have demonstrated that SCP‐2 gene expression appears to be under the control of factors such as adrenocorticotropic hormone and gonadotropins via cyclic adenosine monophosphate (cAMP) activation (Lopez et al. , 2007), which in turn stimulates steroidogenesis (Lopez et al.…”

Section: Introductionmentioning

confidence: 99%

“…The vertebrate SCP-2/x gene transcribes SCP-2 and SCP-x mRNAs via two independent promoters of the gene (Gallegos et al, 2001). Previous studies in SCP-2 transcriptional regulation were conducted in vertebrates (Trzeciak et al, 1987;McLean et al, 1989;Rennert et al, 1991) and these studies have demonstrated that SCP-2 gene expression appears to be under the control of factors such as adrenocorticotropic hormone and gonadotropins via cyclic adenosine monophosphate (cAMP) activation (Lopez et al, 2007), which in turn stimulates steroidogenesis (Lopez et al 2001). It has been demonstrated that both the vertebrate SCP-2 and SCP-x are upregulated in the presence of cAMP and SF-1, a steroidogenic factor, homologous to bFTZ-F1 in insects (Lopez et al, 2007;Lopez et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies in SCP-2 transcriptional regulation were conducted in vertebrates (Trzeciak et al, 1987;McLean et al, 1989;Rennert et al, 1991) and these studies have demonstrated that SCP-2 gene expression appears to be under the control of factors such as adrenocorticotropic hormone and gonadotropins via cyclic adenosine monophosphate (cAMP) activation (Lopez et al, 2007), which in turn stimulates steroidogenesis (Lopez et al 2001). It has been demonstrated that both the vertebrate SCP-2 and SCP-x are upregulated in the presence of cAMP and SF-1, a steroidogenic factor, homologous to bFTZ-F1 in insects (Lopez et al, 2007;Lopez et al 2001). In insects, experiments with AeSCP-2 gene expression regulation demonstrated that addition of 20-hydroxyecdysone (20E) to cultured larval gut tissues increased mRNA levels two fold after a 12 h exposure (Krebs & Lan, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Yellow fever mosquito sterol carrier protein‐2 gene structure and transcriptional regulation

Vyazunova

Lan

2010

Insect Molecular Biology

View full text Add to dashboard Cite

AeSCP-2, a sterol carrier protein, is involved in sterol trafficking in mosquitoes. The activity of the AeSCP-2 gene is important for mosquito development. An earlier study demonstrated that the transcription of this gene was up-regulated by 20-hydroxyecdysone (20E) in cultured gut tissues. To investigate 20E-regulated transcription of AeSCP-2 gene we truncated the upstream flanking region of AeSCP-2 gene and linked it to a reporter gene. The mosquito Aag-2 cell line was transfected with these promoter/reporter constructs and treated with 20E at various concentrations. Expression vectors of different transcription factors such as HR3 and βFTZ-F1 were also co-transfected with the AeSCP-2 promoter/reporter constructs. The observed results demonstrated that varied combinations of transcription factors produce different promoter activities of the AeSCP-2 gene. This observation leads us to the conclusion that the partnership of transcription factors is crucial in regulating transcriptional activity of AeSCP-2 gene.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Yellow fever mosquito sterol carrier protein‐2 gene structure and transcriptional regulation

Vyazunova

Lan

2010

Insect Molecular Biology

View full text Add to dashboard Cite

show abstract

“…Results from the vertebrate SCP-2 gene transcriptional regulation studies have demonstrated that the vertebrate SCP-2 gene expression appears to be under the control of factors such as adrenocorticotropic hormone and gonadotropins via cyclic adenosine monophosphate (cAMP) activation [16]. In insects, 20-hydroxyecdysone (20E) up-regulates the transcription of AeSCP-2 by 2-fold in cultured gut tissues [7], [17].…”

Section: Introductionmentioning

confidence: 99%

THAP and ATF-2 Regulated Sterol Carrier Protein-2 Promoter Activities in the Larval Midgut of the Yellow Fever Mosquito, Aedes aegypti

Peng

Hong

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

Expression of sterol carrier protein-2 (SCP-2) in Aedes aegypti shows a distinct temporal/spatial pattern throughout the life cycle. In order to identify the transcription factors responsible for the larval temporal/spatial regulation of AeSCP-2 transcription, AeSCP-2 promoter activities were studied in vivo via transient transfection of promoter/reporter gene assays. Regulatory sequences upstream −1.3 kb of the transcription start site of AeSCP-2 were found to be critical for the in vivo temporal/spatial promoter activity. Interestingly, the −1.6 kb promoter sequence efficiently drove the larval midgut-specific siRNA expression, indicating that the −1.6 kb upstream sequence is sufficient for temporal/spatial AeSCP-2 transcriptional activity. Four transcription factors were identified in the midgut nuclear extract from feeding larvae via labeled −1.6/−1.3 kb DNA probe pull-down and proteomic analysis. Co-transfection of the promoter/reporter gene with inducible siRNA expression of each transcription factor was performed to confirm the regulatory function of individual transcription factor on AeSCP-2 transcriptional activities in the larval midgut. The results indicate that two of the identified transcription factors, Thanatos-associated protein (THAP) and activating transcription factor-2 (ATF-2), antagonistically control AeSCP-2 transcriptional activity in the midgut of feeding larvae via the regulatory sequences between −1.6 to −1.3 kb 5′ upstream of the transcription start site. In vivo expression knockdown of THAP and ATF-2 resulted in significant changes in developmental progression, which may be partially due to their effects on AeSCP-2 expression.

show abstract

Transcription factor CAAT/enhancer‐binding protein is involved in regulation of expression of sterol carrier protein x in Spodoptera litura

Liang

Zhang

Zeng

et al. 2015

Insect Molecular Biology

View full text Add to dashboard Cite

The Spodoptera litura sterol carrier protein x (SlSCPx) gene is expressed in various tissues throughout the life cycle and plays important role in sterol absorption and transport. In this study, the effects of insect hormones (20-hydroexcdysone and juvenile hormone) and lipids (arachidonic acid, cholesterol) on the expression of SlSCPx was analysed by reverse-transcriptase PCR. The results showed that none of these substances significantly induced the expression of SlSCPx in Spodoptera litura-221 (Spli-221) cells. To identify the transcription factors responsible for regulation of SlSCPx expression, a 3311-bp promoter sequence of the gene was cloned. Transcriptional activity of the promoter was studied using an in vivo promoter/reporter system and a 29-bp sequence between -1000 and -1029 nucleotides (nt) upstream of this gene was found to be responsible for the up-regulation of the gene. Over-expression of CAAT/enhancer-binding protein (C/EBP) in Spli-221 cells increased the promoter activity 5.57-fold. An electrophoretic mobility shift assay showed that two nuclear proteins bound to this sequence. Recombinant C/EBP specifically bound with a putative cis-regulatory element (CRE). Mutation of the C/EBP CRE abolished the binding of the C/EBP with the CRE. These results suggest that the transcription factor C/EBP may regulate the expression of SlSCPx by binding to the CRE in the promoter of this gene.

show abstract

Activation of the SCPx promoter in mouse adrenocortical Y1 cells

Cited by 3 publications

References 21 publications

Yellow fever mosquito sterol carrier protein‐2 gene structure and transcriptional regulation

Yellow fever mosquito sterol carrier protein‐2 gene structure and transcriptional regulation

THAP and ATF-2 Regulated Sterol Carrier Protein-2 Promoter Activities in the Larval Midgut of the Yellow Fever Mosquito, Aedes aegypti

Transcription factor CAAT/enhancer‐binding protein is involved in regulation of expression of sterol carrier protein x in Spodoptera litura

Contact Info

Product

Resources

About