2010
DOI: 10.1242/dev.048652
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Activation of the skeletogenic gene regulatory network in the early sea urchin embryo

Abstract: SUMMARYThe gene regulatory network (GRN) that underlies the development of the embryonic skeleton in sea urchins is an important model for understanding the architecture and evolution of developmental GRNs. The initial deployment of the network is thought to be regulated by a derepression mechanism, which is mediated by the products of the pmar1 and hesC genes. Here, we show that the activation of the skeletogenic network occurs by a mechanism that is distinct from the transcriptional repression of hesC. By me… Show more

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Cited by 63 publications
(90 citation statements)
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References 32 publications
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“…Embryos were fixed (Adomako-Ankomah and Ettensohn, 2011) and WMISH and fluorescent WMISH (F-WMISH) were carried out (Lepage et al, 1992;Duloquin et al, 2007;Sharma and Ettensohn, 2010) as described.…”
Section: Whole-mount In Situ Hybridization (Wmish)mentioning
confidence: 99%
“…Embryos were fixed (Adomako-Ankomah and Ettensohn, 2011) and WMISH and fluorescent WMISH (F-WMISH) were carried out (Lepage et al, 1992;Duloquin et al, 2007;Sharma and Ettensohn, 2010) as described.…”
Section: Whole-mount In Situ Hybridization (Wmish)mentioning
confidence: 99%
“…mRNA for injection was transcribed in vitro using Ambion mMessage mMachine. Concentrations for mRNA injections: 750 ng/μl dominant-negative LvEts1 (DNEts1) (Sharma and Ettensohn, 2010), 250 ng/μl Histone2B-GFP (H2B-GFP), 500 ng/μl H2B-RFP, 750 ng/μl membrane-RFP (mem-RFP) and 500 ng/μl mem-GFP.…”
Section: Transcription Factor Knockdowns and Fluorescent Markersmentioning
confidence: 99%
“…It is important to note, however, that recent data suggest that Alx1 expression is not solely regulated by Pmar1. Sharma and Ettensohn, using pharmacological inhibitors of MEK and WMISH with LvEts1, demonstrated that while Alx1 is activated by Pmar1, its expression is maintained by MAPK signaling and expression of ets1, another transcriptional regulator in the PMC GRN (Sharma and Ettensohn, 2010); thus, ets1 expression under elevated CO 2 conditions should also be considered. The present data contrast with those of Todgham and Hofmann, who observed a decrease in Alx1 expression at 1020matm in prism (~40h.p.f.)…”
Section: Embryo and Micromere Specification Genesmentioning
confidence: 99%