Activation of Toll-like receptors and dendritic cells by a broad range of bacterial molecules

Boele, Linda C. L.; Bajramović, Jeffrey J.; Vries, Anne-Marij M.B.C. de; Voskamp-Visser, Ingrid A.I.; Kaman, Wendy E.; Kleij, Desirée van der

doi:10.1016/j.cellimm.2008.09.002

Cited by 14 publications

(14 citation statements)

References 21 publications

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“…Pemberian dosis LPS sama dengan beberapa penelitian yaitu pada mencit kurang dari 20µg tidak memberikan respon imun pada dengan memberikan LPS secara intraperitoneal dan mencit sehat (24). Penelitian lain juga menyebutkan dilakukan pemeriksaan pada sel makrofag, menunjukan bahwa dosis LPS yang aman untuk binatang coba adalah peningkatan ekspresi TLR2 dibandingkan TLR4 (4,24) berkisar antara 0,25-2,5 µ/g BB (3,25).…”

Section: Dengan Kontrolunclassified

Probiotik Menurunkan Ekspresi TLR2 dan Aktivasi NF-Kb p50 pada Sel Mononuklear Darah Mencit yang T erpajan Lipopolisakarida E.Coli

Sumakto

Santoso

2011

JKB

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ABSTRAKBeberapa tahun terakhir banyak penelitian dilakukan untuk melihat penggunaan probiotik untuk pencegahan maupun untuk pengobatan. Salah satu penggunaan probiotik adalah dalam pengobatan diare. Penggunaan probiotik tersebut berdasarkan respon imun yang dapat ditimbulkan oleh probiotik baik di dalam mukosa usus maupun di sel-sel mononukklear. Penelitian ini bertujuan untuk mengetahui pengaruh probiotik terhadap aktivasi Toll-like receptor (TLR2), TLR4, Nuclear Factor Kappa B p50 dan p65 pada sel mononuklear darah yang terpajan dengan LPS E.coli. Mencit Balb/c dibagi dalam 4 kelompok perlakuan. Satu kelompok mendapat LPS pada hari pertama dilanjutkan dengan probiotik selama 7 hari, satu kelompok LPS saja, satu kelompok probiotik selama 7 hari dan kelompok kontrol tanpa perlakuan. Dilakukan pemeriksaan imunositokimia pada sel mononuklear yang mengekspresikan TLR2, TLR4, dan aktivasi p50 NF-kB dan p65 NF-kB. Hasil penelitian menunjukkankelompok LPS dan probiotik peningkatan ekspresi TLR2 dan TLR4, serta aktivasi NF-kB p50 dan p65, sedangkan pemberian pemberian probiotik mampu menurunkan ekspresi TLR2 dan aktivasi NF-kB p50 (p<0,05) yang disebabkan oleh LPS. Probiotik pada keadaan pajanan dengan LPS E.coli lebih mempengaruhi penurunan ekspresi TLR2 dan menurunkan aktivasi NFkB p50, yang diharapkan mampu menurunkan reaksi inflamasi karena pemberian LPS.Kata Kunci: LPS, NF-kB, probiotik, sel mononuclear, TLR ABSTRACT In recent years much research has been done to investigate use of probiotics for the prevention or treatment. One use of probiotics is for the treatment of diarrhea, where its use is based on an immune response that can be caused by either probiotics in the intestinal mucosa or in mononuclear cells. This study aims to discover the effects of probiotics on the activation of the Toll-like (TLR2), TLR4 receptors, Nuclear Factor

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Section: Dengan Kontrolunclassified

Probiotik Menurunkan Ekspresi TLR2 dan Aktivasi NF-Kb p50 pada Sel Mononuklear Darah Mencit yang T erpajan Lipopolisakarida E.Coli

Sumakto

Santoso

2011

JKB

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“…In monocytes, intact G+ bacteria induce higher levels of IL-12 and TNF production than do G− bacteria, while the latter induce higher levels of IL-10 and PGE 2 [7], [8], [9], [10]. Conversely, human monocytes that have been differentiated in vitro into dendritic cells (DCs) produce similar levels of IL-12 and TNF in response to G+ and G− bacteria [11], [12], [13]. Furthermore, G− bacteria are more potent than G+ bacteria in promoting the up-regulation of co-stimulatory molecules on monocyte-derived DCs [12], [13].…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, human monocytes that have been differentiated in vitro into dendritic cells (DCs) produce similar levels of IL-12 and TNF in response to G+ and G− bacteria [11], [12], [13]. Furthermore, G− bacteria are more potent than G+ bacteria in promoting the up-regulation of co-stimulatory molecules on monocyte-derived DCs [12], [13]. Presentation of unrelated antigens by DC to T cells is known to be enhanced by bacterial-induced maturation, and, in particular G− bacteria, have been shown to promote Th1 polarisation [13], [14], [15].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, G− bacteria are more potent than G+ bacteria in promoting the up-regulation of co-stimulatory molecules on monocyte-derived DCs [12], [13]. Presentation of unrelated antigens by DC to T cells is known to be enhanced by bacterial-induced maturation, and, in particular G− bacteria, have been shown to promote Th1 polarisation [13], [14], [15].…”

Section: Introductionmentioning

confidence: 99%

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Stronger T Cell Immunogenicity of Ovalbumin Expressed Intracellularly in Gram-Negative than in Gram-Positive Bacteria

et al. 2013

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This study aimed to clarify whether Gram-positive (G+) and Gram-negative (G−) bacteria affect antigen-presenting cells differently and thereby influence the immunogenicity of proteins they express. Lactobacilli, lactococci and Escherichia coli strains were transformed with plasmids conferring intracellular ovalbumin (OVA) production. Murine splenic antigen presenting cells (APCs) were pulsed with washed and UV-inactivated OVA-producing bacteria, control bacteria, or soluble OVA. The ability of the APCs to activate OVA-specific DO11.10 CD4+ T cells was assessed by measurments of T cell proliferation and cytokine (IFN-γ, IL-13, IL-17, IL-10) production. OVA expressed within E. coli was strongly immunogenic, since 500 times higher concentrations of soluble OVA were needed to achieve a similar level of OVA-specific T cell proliferation. Furthermore, T cells responding to soluble OVA produced mainly IL-13, while T cells responding to E. coli-expressed OVA produced high levels of both IFN-γ and IL-13. Compared to E. coli, G+ lactobacilli and lactococci were poor inducers of OVA-specific T cell proliferation and cytokine production, despite efficient intracellular expression and production of OVA and despite being efficiently phagocytosed. These results demonstrate a pronounced difference in immunogenicity of intracellular antigens in G+ and G− bacteria and may be relevant for the use of bacterial carriers in vaccine development.

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“…there is therefore an urgent need for new improved adjuvants. the integrated in vitro testing strategy for the development of new adjuvants is comprised of luminescent bioassays for toll-like receptor-mediated responses to assess adjuvanticity (Bsibsi et al, 2010;Boele et al, 2009;Jagessar et al, 2010;Koopman et al, 2013) and 2D/3D in vitro granuloma models to assess adverse effects. Data were shown demonstrating that the sequential use of multiple bioassays as a robust integrated test system can rapidly generate detailed information and can aid the development of new potent adjuvants with minimal adverse effects.…”

Section: Recent Advances In Technology (Crispr/cas9) Now Render It Pomentioning

confidence: 99%

Alternative methods for the use of non-human primates in biomedical research

Burm

Langermans

Bajramović

2014

ALTEX

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Activation of Toll-like receptors and dendritic cells by a broad range of bacterial molecules

Cited by 14 publications

References 21 publications

Probiotik Menurunkan Ekspresi TLR2 dan Aktivasi NF-Kb p50 pada Sel Mononuklear Darah Mencit yang T erpajan Lipopolisakarida E.Coli

Probiotik Menurunkan Ekspresi TLR2 dan Aktivasi NF-Kb p50 pada Sel Mononuklear Darah Mencit yang T erpajan Lipopolisakarida E.Coli

Stronger T Cell Immunogenicity of Ovalbumin Expressed Intracellularly in Gram-Negative than in Gram-Positive Bacteria

Alternative methods for the use of non-human primates in biomedical research

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