Activation of transcription and retrotransposition of a novel retroelement,
            <i>Steamer</i>
            , in neoplastic hemocytes of the mollusk
            <i>Mya arenaria</i>

Arriagada, Gloria; Metzger, Michael J.; Muttray, Annette F.; Sherry, James; Reinisch, Carol L.; Street, Craig; Lipkin, W. Ian; Goff, Stephen P.

doi:10.1073/pnas.1409945111

Cited by 60 publications

(65 citation statements)

References 50 publications

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“…Output from BLAST querying each product from primers Stmr2988F and Stmr3340R to the nr database at NCBI returned high scoring matches only to the canonical Steamer retrotransposon sequence KF319019 (Arriagada et al 2014). Pairwise alignments from BLAST, and multiple sequence alignment (see Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Examination of a variety of bivalves demonstrates that the Steamer retrotransposon, which is known to be present in high copy numbers and is highly expressed in disseminated hemic neoplasia in Mya arenaria (Arriagada et al 2014, Metzger et al 2015, is also present in the genome of other relatively phylogenetically unrelated bivalves including Ensis directus and Macoma balthica. Furthermore, that Steamer is absent from Mya truncata (closely related to, but geographically isolated from, M. arenaria), and from Siliqua patula (closely related to, but geographically isolated from, E. directus) suggests that the distribution of this retrotransposon is not due to ancient common ancestry, but rather due to more recent crossspecies horizontal transmission.…”

Section: Discussionmentioning

confidence: 99%

“…The remainder of the sampled bivalve taxa from AMCC, as well as specimens of Siliqua patula, Mya truncata, and the 2 Dreissena species yielded no product for Steamer, although all yielded cox1 amplification products for the Folmer et al (1994) primers, confirming viability of the DNA isolates. Output from BLAST querying each product from primers Stmr2988F and Stmr3340R to the nr database at NCBI returned high scoring matches only to the canonical Steamer retrotransposon sequence KF319019 (Arriagada et al 2014). Pairwise alignments from BLAST, and multiple sequence alignment (see Fig.…”

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confidence: 99%

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Evidence of horizontal transmission of the cancer-associated Steamer retrotransposon among ecological cohort bivalve species

Paynter

Metzger²,

Sessa³

et al. 2017

Dis. Aquat. Org.

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Bivalve specimens from legacy frozen tissue collections, and others freshly obtained, were surveyed for the presence of the Steamer long terminal repeat (LTR)-retrotransposon associated with disseminated hemic neoplasia of the soft-shelled clam Mya areneria. Of 22 species investigated using primers for the pol region, only Atlantic M. arenaria, Atlantic and North Sea razor clams Ensis directus, and Baltic clams Macoma balthica from the North Sea were found to possess copies of Steamer in their genomes. Notably, close relatives like Mya truncata and Siliqua patula did not exhibit evidence of Steamer. Amplified Steamer sequences were uniformly identical in all M. areneria specimens, and were highly variable across specimens of E. directus. Variation in the latter included nucleotide polymorphisms among and within individuals as well as length variation in 2 specimens corresponding to the deletion of a predicted stable hairpin structure. Results implicate Atlantic razor clams as the proximal source for horizontal transmission of Steamer among ecologically similar yet markedly distantly related bivalves. The consequences of cross-species transmission of the Steamer retrotransposon are unknown, and the finding of Steamer in 3 bivalve species suggests that further spread is possible. KEY WORDS: Disseminated neoplasia · Hemic neoplasia · Retrotransposon · Steamer · Bivalvia · CancerResale or republication not permitted without written consent of the publisher Dis Aquat Org 124: [165][166][167][168] 2017 pression of the Steamer retrotransposon. Steamer is a long terminal repeat (LTR)-retrotrans poson in the Mag family of Ty3/Gypsy elements, complete with a single open reading frame encoding gag and pol genes. Retrotransposons are present in genomic DNA and replicate through reverse transcription of an mRNA transcript into DNA, followed by integration into a new location within the genomic DNA of a cell. As they are notably lacking a retroviral env gene, they are not expected to be able to transfer from cell to cell, as retroviruses do. Similar retrotransposons have been identified in the genomes of 3 other bivalve species known to have transmissible neoplasias, one of which seems to cross the species boundary (Metzger et al. 2016).To date, the Steamer retrotransposon itself is un ique to M. arenaria. Here we examined legacy bio diversity collections and freshly acquired bivalve tissues for the presence of a Steamer signature in their genomes in a manner that might (1) better elucidate whether disseminated neoplasias are ecologically or phylogenetically determined and (2) shed light on the potential for horizontal spread and genomic modification by Steamerlike retrotransposons in the marine environment. MATERIALS AND METHODSBivalve tissue samples were obtained from the American Museum of Natural History's Ambrose Monell Cryo Collection (AMCC), and included 22 species from the North Atlantic (see Table S1 in the Supplement at www. int-res. com/ articles/ suppl/ d124 p165 _ supp. pdf). Fresh specimens of steam...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Evidence of horizontal transmission of the cancer-associated Steamer retrotransposon among ecological cohort bivalve species

Paynter

Metzger²,

Sessa³

et al. 2017

Dis. Aquat. Org.

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“…Recently, Arriagada et al (2014) have identified and characterized a novel retrotransposon named Steamer with an expression strongly associated with DN in M. arenaria, which constitutes a new molecular marker of the disease in softshell clams.…”

Section: Molecular Techniquesmentioning

confidence: 99%

Neoplastic diseases of marine bivalves

Carballal

Barber

Iglesias

et al. 2015

Journal of Invertebrate Pathology

116

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“…This equilibrium may also be threatened by distinct sensitivity to diseases, such a leukemia. This epidemic transmissible disease is caused by the insertion of retroviral sequences in the genome of the mussels and has recently been shown to spread horizontally [35,36]. It is well known to affect many bivalves species, including Mya arenaria and Mytilus spp.…”

Section: Population Dynamicsmentioning

confidence: 99%

Biomonitoring Climate Change and Pollution in Marine Ecosystems: A Review onAulacomya ater

Caza

Cledón

St‐Pierre

2016

Journal of Marine Biology

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The sedentarism and wide global distribution of the blue musselMytilus edulishave made it a useful bioindicator to assess changes in the health status of the marine ecosystem in response to pollution and other environmental stresses. Effective biomonitoring of an ecosystem requires, however, that multiple biomarkers be used to obtain an accurate measure of the cumulative effects of different sources of environmental stress. Here, we provide a first integrated review of the biological, economical, and geographical characteristics of another species of mussels, the ribbed musselAulacomya ater. We discuss the use ofAulacomya ateras a complementary biomonitor to the blue mussel to assess the impact of pollutants and climate change. Recent findings have indeed shown thatMytilus edulisandAulacomya aterhave distinctive anatomy and physiology and respond differently to environmental stress. Monitoring of mixed beds containing blue and ribbed mussels may thus represent a unique opportunity to study the effect of environmental stress on the biodiversity of marine ecosystems, most notably in the Southern hemisphere, which is particularly sensitive to climate change and where both species often cohabitate in the same intertidal zones.

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Activation of transcription and retrotransposition of a novel retroelement, Steamer , in neoplastic hemocytes of the mollusk Mya arenaria

Cited by 60 publications

References 50 publications

Evidence of horizontal transmission of the cancer-associated Steamer retrotransposon among ecological cohort bivalve species

Evidence of horizontal transmission of the cancer-associated Steamer retrotransposon among ecological cohort bivalve species

Neoplastic diseases of marine bivalves

Biomonitoring Climate Change and Pollution in Marine Ecosystems: A Review onAulacomya ater

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