Activation Tagging in Arabidopsis

Weigel, Detlef; Ahn, Ji Hoon; Blázquez, Miguel Á.; Borevitz, Justin O.; Christensen, S.; Fankhauser, Christian; Ferrándiz, Cristina; Kardailsky, Igor; Malancharuvil, Elizabeth J.; Neff, Michael M.; Nguyen, Jasmine; Sato, Shusei; Wang, Wenfei; Xia, Yiji; Dixon, Richard A.; Harrison, Maria J.; Lamb, Chris; Yanofsky, Martin F.; Chory, Joanne

doi:10.1104/pp.122.4.1003

Cited by 874 publications

(902 citation statements)

References 53 publications

Supporting

Mentioning

877

Contrasting

Unclassified

Order By: Relevance

“…DET2 encodes a steroid 5a-reductase that catalyzes a key step in BR biosynthesis (Noguchi et al, 1999). This close proximity raised the possibility that the large-leaf phenotype we attributed to a disruption of PHT4;2 was instead caused by an overexpression of DET2 via T-DNA activation (Weigel et al, 2000;Ren et al, 2004). However, this possibility is inconsistent with our finding that the large-leaf phenotype (assessed by rosette fresh weight) segregated as a recessive trait in F2 progeny of a cross between the wild type and the homozygous mutant (14 wild type [1.69 6 0.06 g]:45 hemizygous mutant [1.56 6 0.10 g]:15 homozygous mutant [2.17 6 0.12 g]; x 2 = 3.48, P = 0.17).…”

Section: Plant Size and Biomassmentioning

confidence: 99%

The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis

et al. 2011

View full text Add to dashboard Cite

Nonphotosynthetic plastids are important sites for the biosynthesis of starch, fatty acids, and amino acids. The uptake and subsequent use of cytosolic ATP to fuel these and other anabolic processes would lead to the accumulation of inorganic phosphate (Pi) if not balanced by a Pi export activity. However, the identity of the transporter(s) responsible for Pi export is unclear. The plastid-localized Pi transporter PHT4;2 of Arabidopsis (Arabidopsis thaliana) is expressed in multiple sink organs but is nearly restricted to roots during vegetative growth. We identified and used pht4;2 null mutants to confirm that PHT4;2 contributes to Pi transport in isolated root plastids. Starch accumulation was limited in pht4;2 roots, which is consistent with the inhibition of starch synthesis by excess Pi as a result of a defect in Pi export. Reduced starch accumulation in leaves and altered expression patterns for starch synthesis genes and other plastid transporter genes suggest metabolic adaptation to the defect in roots. Moreover, pht4;2 rosettes, but not roots, were significantly larger than those of the wild type, with 40% greater leaf area and twice the biomass when plants were grown with a short (8-h) photoperiod. Increased cell proliferation accounted for the larger leaf size and biomass, as no changes were detected in mature cell size, specific leaf area, or relative photosynthetic electron transport activity. These data suggest novel signaling between roots and leaves that contributes to the regulation of leaf size.

show abstract

Section: Plant Size and Biomassmentioning

confidence: 99%

The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The ztl-3 (31), ztl21 (29), fkf1 (37), cry1cry2 (38), cry1cry2phyA (39), cry1cry2phot1phot2 (40), and cop1-4 (41) mutants have been described. Transgenic Arabidopsis lines were prepared by floral dip transformation method (42,43). Phenotypes of transgenic plants were verified in at least three independent transgenic lines.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis CRY2 and ZTL mediate blue-light regulation of the transcription factor CIB1 by distinct mechanisms

Liu

Wang

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Significance Plants rely on many different types of photoreceptors to control light responses, but the molecular mechanisms underlying the coaction of different photoreceptors that regulate the same light response remains unclear. This study demonstrates a mechanism of the photoreceptor coaction. In Arabidopsis , the blue-light receptor cryptochrome 2 interacts with and activates the transcription factor CRYPTOCHROME-INTERACTING basic helix–loop–helix 1 (CIB1). It was found that CIB1 protein is degraded in the absence of blue light and that the light–oxygen–voltage-domain F-box blue-light receptor ZEITLUPE mediates blue-light suppression of CIB1 degradation.

show abstract

“…The yid1 mutant was screened from the T-DNA insertional mutant collection (Weigel et al, 2000;Qin et al, 2003). The mutants sfr6-2 and med25-2 were described previously (Knight et al, 2009;Xu and Li, 2011).…”

Section: Experimental Procedures Plant Growth Conditions and Iron-defmentioning

confidence: 99%

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

et al. 2014

View full text Add to dashboard Cite

These authors contributed equally to this work. SUMMARYIron is an essential micronutrient for plants and animals, and plants are a major source of iron for humans. Therefore, understanding the regulation of iron homeostasis in plants is critical. We identified a T-DNA insertion mutant, yellow and sensitive to iron-deficiency 1 (yid1), that was hypersensitive to iron deficiency, containing a reduced amount of iron. YID1 encodes the Arabidopsis Mediator complex subunit MED16. We demonstrated that YID1/MED16 interacted with another subunit, MED25. MED25 played an important role in regulation of iron homeostasis by interacting with EIN3 and EIL1, two transcription factors in ethylene signaling associated with regulation of iron homeostasis. We found that the transcriptome in yid1 and med25 mutants was significantly affected by iron deficiency. In particular, the transcription levels of FIT, IRT1 and FRO2 were reduced in the yid1 and med25 mutants under iron-deficient conditions. The finding that YID1/MED16 and MED25 positively regulate iron homeostasis in Arabidopsis increases our understanding of the complex transcriptional regulation of iron homeostasis in plants.

show abstract

Activation Tagging in Arabidopsis

Cited by 874 publications

References 53 publications

The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis

The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis

Arabidopsis CRY2 and ZTL mediate blue-light regulation of the transcription factor CIB1 by distinct mechanisms

The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25

Contact Info

Product

Resources

About