Activation tagging in <i>indica</i> rice identifies ribosomal proteins as potential targets for manipulation of water‐use efficiency and abiotic stress tolerance in plants

Moin, Mazahar; Bakshi, Achala; Saha, Anusree; Kumar, Manish; Reddy, A. Ramachandra; Rao, K. V.; Siddiq, E. A.; Kirti, Pulugurtha Bharadwaja

doi:10.1111/pce.12796

Cited by 45 publications

(68 citation statements)

References 57 publications

(70 reference statements)

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“…RPL6 and RPL23A, in addition to their role in WUE, became up-regulated in almost all the stresses studied illustrating their possible involvement in inducing tolerance to abiotic stresses (Moin et al, 2016). Cold, MeJa, and SA treatments induced the up-regulation of a majority of RPL genes, while H 2 O 2 and heat treatments down-regulated 75% of the genes.…”

Section: Discussionmentioning

confidence: 99%

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

et al. 2016

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Ribosomal proteins (RPs) are well-known for their role in mediating protein synthesis and maintaining the stability of the ribosomal complex, which includes small and large subunits. In the present investigation, in a genome-wide survey, we predicted that the large subunit of rice ribosomes is encoded by at least 123 genes including individual gene copies, distributed throughout the 12 chromosomes. We selected 34 candidate genes, each having 2–3 identical copies, for a detailed characterization of their gene structures, protein properties, cis-regulatory elements and comprehensive expression analysis. RPL proteins appear to be involved in interactions with other RP and non-RP proteins and their encoded RNAs have a higher content of alpha-helices in their predicted secondary structures. The majority of RPs have binding sites for metal and non-metal ligands. Native expression profiling of 34 ribosomal protein large (RPL) subunit genes in tissues covering the major stages of rice growth shows that they are predominantly expressed in vegetative tissues and seedlings followed by meiotically active tissues like flowers. The putative promoter regions of these genes also carry cis-elements that respond specifically to stress and signaling molecules. All the 34 genes responded differentially to the abiotic stress treatments. Phytohormone and cold treatments induced significant up-regulation of several RPL genes, while heat and H2O2 treatments down-regulated a majority of them. Furthermore, infection with a bacterial pathogen, Xanthomonas oryzae, which causes leaf blight also induced the expression of 80% of the RPL genes in leaves. Although the expression of RPL genes was detected in all the tissues studied, they are highly responsive to stress and signaling molecules indicating that their encoded proteins appear to have roles in stress amelioration besides house-keeping. This shows that the RPL gene family is a valuable resource for manipulation of stress tolerance in rice and other crops, which may be achieved by overexpressing and raising independent transgenic plants carrying the genes that became up-regulated significantly and instantaneously.

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Section: Discussionmentioning

confidence: 99%

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

et al. 2016

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“…TOR is a highly conserved master coordinator of nutrients, energy and stress signaling in the cell (Xiong and Sheen, 2014). Thus, implying a role of RPs in the direct response to stress, through a specific network of genes related to stress in plants, such as defense proteins, ROS response genes, genes of calcium signaling and the vast network of genes associated with phytohormones (Moin et al, 2016). Finally, given the induction of RPS6 in the vanilla transcriptome in response to Fusarium , this may suggest the participation of TOR master regulator in the translational regulation in the Vanilla- Fusarium pathosystem.…”

Section: Discussionmentioning

confidence: 99%

Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

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Adame-García

Gregorio-Jorge

et al. 2019

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BackgroundUpon exposure to unfavorable environmental conditions, plants need to respond quickly to maintain their homeostasis. For instance, physiological, biochemical and transcriptomical changes must occur during interactions with pathogens, this causing the triggering of pathogen- and plant-derived molecules. In the case of Vanilla planifolia Jacks., a worldwide economically important crop, it is susceptible to Fusarium oxysporum f. sp. vanillae. This pathogen causes root and stem rot in vanilla plants that finally leads to plant death. To further investigate how vanilla plants respond at the transcriptional level upon infection with F.oxysporum f. sp. vanillae, we employed the RNA-Seq approach to analyze the dynamics of whole-transcriptome changes during two-time frames of the infection.ResultsAnalysis of global gene expression profiles indicated that a major transcriptional change occurs at 2 dpi, in comparison to 10 dpi, whereas 3420 genes were found with a differential expression at 2 dpi, only 839 were identified at 10 dpi. The analysis of the transcriptional profile at 2 dpi suggests that vanilla plants prepare to counter the infection by gathering a pool of translational regulation-related transcripts.ConclusionsWe propose that the plant-pathogen interaction at early stages causes a transcriptional reprogramming coupled with a translational regulation. Altogether, this study provides the identification of molecular players that could help to fight the most damaging disease of vanilla, where ribosomal proteins and regulation of the translational mechanism are critical. These are insights into the defense responses of V. planifolia Jacks., providing the basis for the understanding of the plant early response towards biotic stress.

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Ribosomal Protein Large subunit RPL6 modulates salt tolerance in rice

Moin

Saha

Bakshi

et al. 2020

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1The extra-ribosomal functions of ribosomal proteins RPL6 and RPL23a in stress-2 responsiveness have emanated from our previous studies on activation tagged mutants of rice 3 screened for water-use efficiency (Moin et al., 2016a). In the present study, we functionally 4 validated the RPL6, a Ribosomal Protein Large subunit member for salt stress tolerance in 5 rice. The overexpression of RPL6 resulted in tolerance to moderate (150 mM) to high (200 6 mM) levels of salt (NaCl) in rice. The transgenic rice plants expressing RPL6 constitutively 7 showed better phenotypic and physiological responses with high quantum efficiency, 8 accumulation of more chlorophyll and proline contents, and an overall increase in seed yield 9 compared with the wild type in salt stress treatments. An iTRAQ-based comparative 1 0 proteomic analysis revealed the high expression of about 333 proteins among the 4,378 DEPs

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Activation tagging in indica rice identifies ribosomal proteins as potential targets for manipulation of water‐use efficiency and abiotic stress tolerance in plants

Cited by 45 publications

References 57 publications

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

Ribosomal Protein Large subunit RPL6 modulates salt tolerance in rice

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