Activation tagging in Salvia miltiorrhiza can cause increased leaf size and accumulation of tanshinone I and IIA in its roots

Ho, Hsin Shueh; Vishwakarma, Rishi K.; Chen, Emily Chin Fun; Tsay, Hsin Sheng

doi:10.1186/1999-3110-54-37

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…Inconsistent with Min-hui et al [13] results, Sal A was also found in all of the investigated Salvia species of Iran, except for the roots of S. macrosiphon. Furthermore, in our study the maximum value of Sal A (8.10 mg/g DW) was obtained in the leaves of S. verticillata (IBRC), which was approximately twenty times higher than that previously reported for the commercial roots of S. miltiorrhiza by Ho et al [48].…”

Section: Totalcontrasting

confidence: 68%

“…In accordance with the Chinese Pharmacopoeia (2010), Sal B is an index and chemical marker for the quality control of Danshen, dried roots of S. miltiorrhiza, and its content should not be less than 30 mg/g DW (3%) [47]. Based on the extraction solvent, the highest amount for Sal A has been reported as 0.41 mg/g DW in the roots of commercially available S. miltiorrhiza plants [48]. Inconsistent with Min-hui et al [13] results, Sal A was also found in all of the investigated Salvia species of Iran, except for the roots of S. macrosiphon.…”

Section: Totalmentioning

confidence: 81%

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HPLC Fingerprint of Important Phenolic Compounds in Some Salvia L. Species from Iran

Fotovvat¹,

Radjabian²,

Saboora³

2018

Rec.Nat.Prod.

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Salvia L. is a large genus of the Lamiaceae family with high medicinal value. Pharmaceutical properties of Salvia species are mainly due to their secondary metabolites, especially phenolic compounds. This study was focused on identification and determination of five bioactive phenolic compounds (rosmarinic acid, carnosic acid, caffeic acid, salvianolic acids A and B) in the 41 populations from 27 wild Salvia species of Iran using a simple and reliable HPLC-UV method. The principal component analysis (PCA) technique was used to study differentiation among species according to their phenolic compound profiles. Significant intra-and interspecific variations were observed in the distribution patterns and contents of phenolic compounds in the studied Salvia species. As a result of this study, it was found that leaves had greater amounts of phenolic compounds as compared to the roots. The highest content of rosmarinic acid (41.53±0.88 mg/g DW) and salvianolic acid A (8.10±0.35 mg/g DW) were found in the leaves of S. verticillata. The leaves of S. syriaca and S. sharifii were rich in salvianolic acid B (54.47±2.00 mg/g DW) and carnosic acid (34.05±1.18 mg/g DW), respectively. The PCA results revealed chemical variations in the Salvia species collected from different regions and could fully distinguish between them based on the phenolic compounds concentrations. The present study demonstrated that apart from S. officinalis, some wild species such as S. verticillata, S. hypoleuca, S. leriifolia and S. virgata can be introduced as potent natural sources for medicinal and industrial purposes.

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Section: Totalcontrasting

confidence: 68%

Section: Totalmentioning

confidence: 81%

HPLC Fingerprint of Important Phenolic Compounds in Some Salvia L. Species from Iran

Fotovvat¹,

Radjabian²,

Saboora³

2018

Rec.Nat.Prod.

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“…No band was observed in the non-transformed control plant (Data not shown). Single or low-copy integration of transgenes through A. tumefaciens have been observed in several plant species (Tsay et al 2012;Ho et al 2013).…”

Section: Molecular Analysismentioning

confidence: 99%

Efficient shoots regeneration and genetic transformation of Bacopa monniera

Kumari

Vishwakarma

Gupta

et al. 2015

Physiol Mol Biol Plants

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Bacopa monniera is an important source of metabolites with pharmaceutical value. It has been regarded as a valuable medicinal plant and its entire commercial requirement is met from wild natural population. Recently, metabolic engineering has emerged as an important solution for sustained supply of assured and quality raw material for the production of active principles. Present report describes efficient in vitro multiplication and transformation method for genetic manipulation of this species. MS medium supplemented with 2 mgl −1 BA and 0.2 mgl −1 IAA was found optimum for maximum shoot regeneration (98.33 %) from in vitro leaves with 2-3 longitudinal cuts. Agrobacterium tumefaciens-mediated transformation method was used for generating transgenic B. monniera plants. Putative transformants were confirmed by GUS assay and PCR based confirmation of hptII gene. DNA blot analysis showed single copy insertion of transgene cassette. An average of 87.5 % of the regenerated shoots were found PCR positive for hptII gene and GUS activity was detected in leaves of transgenic shoots at a frequency of 82.5 % The efficient multiple shoots regeneration system described herein may help in mass production of B. monniera plant. Also, the high frequency transformation protocol described here can be used for genetic engineering of B. monniera for enhancement of its pharmaceutically important metabolites.

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“…Whatʼs more, other active constituents are also bioactive. Tanshinone I has activity in the treatment of angina pectoris [12], dihydrotanshinone I shows an inhibitory effect on the induction of apoptosis and action of DNA topoisomerase I [13,14], and rosmarinic acid also has shown biological activities, such as protection of the liver, and inhibiting antitumor, HIV-I, antihepatitis, blood clots, and anti-inflammation [15,16]. In recent years, there has been a mounting demand for S. miltiorrhiza because of its potent Abstract !…”

mentioning

confidence: 99%

Spaceflight Environment-Induced Variation in Root Yield and Active Constituents of Salvia miltiorrhiza

Peng

Wang

et al. 2014

Planta Med

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Salvia miltiorrhiza is a significant source of bioactive compounds providing human health effects. Here, we surveyed root yield and the active constituents' divergences of second generation S. miltiorrhiza (SP2) responding to a spaceflight environment. High-performance liquid chromatography was conducted for the comprehensive constituents' characterizations of 28 SP2 lines (224 individuals) and the ground control (eight individuals). The results showed that the mean fresh and dry weight of roots ranged from 116 to 172 g and 25 to 119 g, respectively, in SP2 lines. In addition, the mean contents of four tanshinone compounds (tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone I) of 28 SP2 lines varied from 0.32 to 1.04 mg · g(-1), 0.47 to 2.39 mg · g(-1), 0.25 to 1.60 mg · g(-1), and 0.53 to 1.67 mg · g(-1), respectively. Except for salvianolic acid B, which varied drastically from 72 % to 201 % of the ground control treatment, the other six phenolic acid contents of the 28 SP2 lines all increased after spaceflight. Principal component analysis was performed to obtain an overview of the distribution of all samples, and score plots clearly separated the SP2 accessions from ground controls. Moreover, a positive relationship was observed between tanshinone I and tanshinone IIA (r = 0.790, p < 0.01), and rosmarinic acid was positively correlated with salvianolic acid B (r = 0.728, p < 0.01). In conclusion, this study demonstrated that a spaceflight environment induced SP2 accessions remarkably in the variation of root yield and active constituent content.

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Activation tagging in Salvia miltiorrhiza can cause increased leaf size and accumulation of tanshinone I and IIA in its roots

Cited by 8 publications

References 41 publications

HPLC Fingerprint of Important Phenolic Compounds in Some Salvia L. Species from Iran

HPLC Fingerprint of Important Phenolic Compounds in Some Salvia L. Species from Iran

Efficient shoots regeneration and genetic transformation of Bacopa monniera

Spaceflight Environment-Induced Variation in Root Yield and Active Constituents of Salvia miltiorrhiza

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