Active Cutaneous Anaphylaxis (ACA) in the Mouse Ear

Abstract

ABSTRACT-Active cutaneous anaphylaxis (ACA) was studied in the ear of female BALB/c mice. Mice were immunized with ovalbumin in the presence of aluminium hydroxide gel or complete Freund's adjuvant (CFA). Two weeks after the immunization, ACA was elicited in the mouse ear by injecting 10,ul of antigen solution intradermally into the ear lobe. ACA was assessed by the amount of extravasated dye, which was given intravenously just after the antigen injection. Antiallergic drugs (tra nilast, ketotifen and azelastine), antihistamines (chlorpheniramine, diphenhydramine and mequitazine), fl-stimulants (isoproterenol and salbutamol), theophylline and glucocorticoids (hydrocortisone, predni solone and dexamethasone) inhibited the reaction significantly. These drugs inhibited both ACA in mice immunized with alum-precipitated antigen and ACA in mice injected with CFA-emulsified antigen similarly. ACA in the mouse ear might be a useful tool for studying drugs for allergy.Keywords: Active cutaneous anaphylaxis, Ear (mouse), Antiallergic drug, IgE antibody, IgG1 antibody Passive cutaneous anaphylaxis (PCA) (1, 2) is a use ful experimental model for studying drugs for allergy (3 6). The rat has been the most useful species for this purpose and the reaction is assessed by measuring the amount of extravasated dye (7,8). On the other hand, the mouse is the most established experimental animal and there are many strains of mice with unique im munological characteristics (9-12).Furthermore, Feinberg (13) reported the suitability of mouse ear for induction of PCA, and Prouvost-Danon and Binaghi (14) demonstrated that mouse ear had higher reactivity for PCA than mouse dorsal skin. According to these re ports, we established a method for PCA in the mouse ear by assessing the amount of extravasated dye (15) and characterized it pharmacologically and immunologi cally (16-23). However, although passive sensitization provides a simple model that is suitable for analyzing the mechanisms involved in both allergic reactions and drug action, active sensitization is also important for studying allergic diseases, because allergic diseases are caused by active sensitization. In the present study, therefore, we applied the technique of mouse ear PCA to active cutaneous anaphylaxis (ACA) elicited in female BALB/c mice, and examined the effect of drugs which are used for allergic diseases on the ACA. MATERIALS AND METHODS MiceFemale BALB/c mice were used for ACA and male ddY mice were used for titration of serum antibodies. Both mice were purchased from Japan SLC, Inc. (Hamamatsu, Japan). Antigen and adjuvantsOvalbumin (OA, 5 times crystalized) was purchased from Seikagaku Kogyo Co., Ltd. (Tokyo, Japan). As adjuvants, aluminium hydroxide gel (alum) and com plete Freund's adjuvant (CFA) were used. Alum was prepared according to the method of Levine and Vaz (24), and CFA was obtained from Nacalai Tesque, Inc. (Kyoto, Japan). ACATen-weeks-old female BALB/c mice were immunized with OA in the presence of an adjuvant. To induce IgE antibody mainly, 1,ug...

Orally supplemented Lactobacillus acidophilus strain L‐92 inhibits passive and active cutaneous anaphylaxis as well as 2,4‐dinitroflurobenzene and mite fecal antigen induced atopic dermatitis‐like skin lesions in mice

“…Con, control (sensitized mice); L-92: L-92-treated sensitized mice; Tac, tacrolimus-treated sensitized mice; Veh, vehicle (non-sensitized mice). gic reactions, active sensitization plays a more significant role in the pathogenesis of allergic diseases (31). However, similar to the PCA response, L-92 potentially inhibited the vascular permeability increase caused by ACA and production of OVA-specific IgE in comparison with the control mice.…”

“…Therefore, L‐92 may suppress IgE‐dependent mast cell activation and/or antagonize against histamine action. Although passive sensitization is suitable for analysis of allergic reactions, active sensitization plays a more significant role in the pathogenesis of allergic diseases (31). However, similar to the PCA response, L‐92 potentially inhibited the vascular permeability increase caused by ACA and production of OVA‐specific IgE in comparison with the control mice.…”

Effect of Protease-Activated Receptor-2 Deficiency on Allergic Dermatitis in the Mouse Ear

“…Furthermore, participation of mast cells in CH has been suggested (44), and a defective CH was demonstrated in mast cell-deficient mice (45). Because activation of mast cells is important in PCA and the degree of PCA can be estimated by measuring the amount of extravasated dye in the reaction site (31,32,46,47), we examined the effect of PAR-2 deficiency on the increased dye content after ear PCA. As shown in Fig.…”

Stigmasterol Alleviates Cutaneous Allergic Responses in Rodents

“…The cutaneous antigen challenge of previously sensitized mice triggers mast cell activation and degranulation, release of vasoactive mediators, increase in vascular permeability, and dye extravasation. Mast cell stabilizers like sodium cromoglycate, antihistamines (H 1 receptor blockers), and glucocorticoids given over longer periods have been found to reduce this allergic reaction [ 36 ]. In the classic ACA study by Inagaki et al (1992), mast cell stabilizers and antihistamines were shown to be effective when given in a short period while steroids showed effect when given 8 h prior to allergen challenge.…”