Active matrix metalloproteinase-8 (aMMP-8) point-of-care test (POCT) in the COVID-19 pandemic

Sorsa, Timo; Sahni, Vaibhav; Buduneli, Nurcan; Gupta, Shipra; Räisänen, Ismo T.; Golub, Lorne M.; Lee, Hsi‐Ming; Pätilä, Tommi; Bostancı, Nagihan; Meurman, Jukka H.; Pärnänen, Pirjo; Nwhator, Solomon O.; Singla, Mohita; Gauba, Krishan

doi:10.1080/14789450.2021.1976151

Cited by 30 publications

(44 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since the start of the pandemic in 2019, our team has been working diligently towards unravelling the intricacies of this association [ 2 – 6 ]. An association between periodontitis and higher risk of Intensive Care Unit admission, need for ventilation and death of COVID-19 patients, is now well documented [ 3 ].…”

mentioning

confidence: 99%

“…Hence any diagnostic modality which could predictably screen the presence of periodontal disease could very well act as an alarm to encourage and motivate the patients to seek dental care before it has progressed to severe stage. Recently developed and validated active matrix metalloproteinase (aMMP)-8 point-of-care/chair-side biomarker technology provides possibilities for health care professionals in this regard [ 6 ]. These lateral flow immunoassay based kits are capable of diagnosing active periodontal disease at both full-mouth and site-specific levels and can aid in tailoring therapy as per patient requirements and at the same time sensitizing them towards the need to maintain oral health [ 6 ].…”

mentioning

confidence: 99%

“…Recently developed and validated active matrix metalloproteinase (aMMP)-8 point-of-care/chair-side biomarker technology provides possibilities for health care professionals in this regard [ 6 ]. These lateral flow immunoassay based kits are capable of diagnosing active periodontal disease at both full-mouth and site-specific levels and can aid in tailoring therapy as per patient requirements and at the same time sensitizing them towards the need to maintain oral health [ 6 ].

Fig.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Poor oral health may prolong COVID-19 illness

et al. 2022

Self Cite

View full text Add to dashboard Cite

mentioning

confidence: 99%

mentioning

confidence: 99%

Fig.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Poor oral health may prolong COVID-19 illness

et al. 2022

Self Cite

View full text Add to dashboard Cite

“…According to Sorsa et al, the active metalloproteinase-8 point-of-care test (aMMP-8 POCT) provides a simple, Non-invasive, rapid, and real-time tool that may be used to identify the Peri-implant disease as a potential risk factor of COVID-19 [ 63 ]. The aMMP-8 levels above 20 ng/ml are indicative of active Peri-implant disease.…”

Section: Resultsmentioning

confidence: 99%

Impact of COVID-19 on Periodontitis and Peri-Implantitis: A Narrative Review

Mancini¹,

Americo²,

Pizzolante³

et al. 2022

Front. Oral. Health

View full text Add to dashboard Cite

COVID-19 is reported as one of the most widespread diseases in the world. An extraordinary number of articles and manuscripts have focused on the inflammatory cascade and sequelae, showing the important roles of cytokines and renin-angiotensin levels and possible links to other pathologies. Nowadays, interest regarding the possible correlation between COVID-19 and periodontal and Peri-implant diseases is increasing. This mini-review aims to collect data regarding the possible correlation between COVID-19 and periodontitis or Peri-implantitis through the analysis of articles published in the last 3 years. The following keywords were used: ([periodontitis OR periodontal disease] AND [COVID-19]); ([Peri-implantitis OR mucositis] AND [COVID-19 OR Sars-CoV-2]). The inclusion criteria were studies on COVID-19 or SARS-CoV-2 and periodontitis or Peri-implantitis, and studies on the molecular and cellular aspect of COVID-19 in periodontal or Peri-implant tissues. The search revealed 484 articles in total (PubMed 208 and Scopus 276). After a screening of titles and abstracts, 47 articles were included in the full-text analysis. Two articles comprised the Peri-implant group: a short communication and a review. Regarding the periodontal group, 45 articles were selected and analyzed according to the type of study, population, and aim. Of these, 10 articles were clinical studies, and the other 35 were hypotheses, reviews, letters to the editor, or commentaries. In conclusion, according to the data extracted, a mutual correlation between COVID-19 and periodontitis can be stated; however, data linked to Peri-implantitis are still missing, and future clinical studies are still needed.

show abstract

“…The visible result on the dipstick was documented with a photograph too. Among the positive cases with two lines there existed both weak or thin(ner) (a weak positive) or strong or thick(er) (a strong positive) second line (Sorsa et al, 2021 ). The remaining elution fluid of PISF was used for further analysis in the study and was analyzed for MMP‐2 and MMP‐9 as well as total MMP‐8, IL‐6, and calprotectin by Western immunoblotting, gelatin‐zymography, and ELISA as described in Sections 2.2.2 , 2.2.3 , 2.2.4 .…”

Section: Methodsmentioning

confidence: 99%

Active MMP‐8 point‐of‐care (PoC)/chairside enzyme‐test as an adjunctive tool for early and real‐time diagnosis of peri‐implantitis

Lähteenmäki

Tervahartiala

Räisänen

et al. 2022

Clinical & Exp Dental Res

Self Cite

View full text Add to dashboard Cite

Objective The aim of this study was to investigate the utility of the active matrix metalloproteinase (aMMP‐8)‐point‐of‐care (PoC) test as a quantitative real‐time chair‐side diagnostic tool for peri‐implant diagnosis, as well as assess the potentially developing and ongoing risk relative to the traditional clinical methods. Background Current peri‐implant and periodontal disease diagnoses rely on clinical and radiological examinations. This case‐control study investigated the applicability of aMMP‐8‐PoC immunotest for quantitative real‐time diagnosis and monitoring of dental implants in health and disease. Methods Sixty‐eight patients visiting a specialist clinic for maintenance following dental implant placement underwent assessment of their peri‐implant health. aMMP‐8‐PoC peri‐implant sulcular fluid (PISF) lateral‐flow immunotests were performed using ImplantSafe® technology quantitated by ORALyzer®. In addition, the PISF samples were analyzed for total MMP‐8, calprotectin, and interleukin (IL)‐6 by enzyme‐linked immunosorbent assays (ELISA), aMMP‐8 by western immunoblot, and MMP‐2 and MMP‐9 by gelatin zymography. Results The aMMP‐8‐PoC test promptly recorded and reflected peri‐implant disease, differentiating it clearly from health. X‐ray findings (bone loss > 2 mm), peri‐implant pocket depth ≥ 3 mm, and bleeding on probing were significantly more prevalent among implants positive for the aMMP‐8‐PoC test. aMMP‐8/ORALyzer analysis was more precise in recording disease than total MMP‐8, calprotectin, IL‐6, MMP‐2, and MMP‐9. Conclusions The aMMP‐8‐PoC test can be conveniently implemented to alert for and detect active collagenolysis affecting peri‐implant tissues, both in the early and advanced stages of the disease. Active and fragmented MMP‐8 exhibits a strong and significant association with peri‐implantitis as compared to total MMP‐8 and other biomarkers and can be utilized as the POC/chairside biomarker of choice in the new classification of peri‐implantitis.

show abstract

Active matrix metalloproteinase-8 (aMMP-8) point-of-care test (POCT) in the COVID-19 pandemic

Cited by 30 publications

References 98 publications

Poor oral health may prolong COVID-19 illness

Poor oral health may prolong COVID-19 illness

Impact of COVID-19 on Periodontitis and Peri-Implantitis: A Narrative Review

Active MMP‐8 point‐of‐care (PoC)/chairside enzyme‐test as an adjunctive tool for early and real‐time diagnosis of peri‐implantitis

Contact Info

Product

Resources

About