Active microrheology using pulsed optical tweezers to probe viscoelasticity of lamin A

Mukherjee, C. D.; Kundu, Avijit; Dey, Raunak; Banerjee, Ayan; Sengupta, Kaushik

doi:10.1039/d1sm00293g

Cited by 11 publications

(9 citation statements)

References 49 publications

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“…normal stress coefficient and fluid relaxation time) that are difficult to be measured using a typical rheometer. 4,5,6,[21][22][23] A detailed understanding of the viscoelastic lift on a particle in a confined flow is vital to the design and research for the aforementioned applications. In general, the combined effects of particle properties (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Experimental and theoretical studies of cross-stream migration of non-spherical particles in a quadratic flow of a viscoelastic fluid

Tai¹,

Narsimhan²

2022

Soft Matter

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Section: Introductionmentioning

confidence: 99%

“…normal stress coefficient and fluid relaxation time) that are difficult to be measured using a typical rheometer. 4,5,6,21–23…”

Section: Introductionmentioning

confidence: 99%

Experimental and theoretical studies of cross-stream migration of non-spherical particles in a quadratic flow of a viscoelastic fluid

Tai¹,

Narsimhan²

2022

Soft Matter

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“…Mutation of A350P in lamin A principally affects cardiac muscles and leads to dilated cardiomyopathy. In a 2021 study, a pulsed optical tweezer was used to detect the viscosity parameters of wild-type and A350P lamin A, and it was found that the complex viscosity for A350P lamin A was higher than that of wild-type lamin A, which may translate into nuclear plasticity ( Mukherjee et al, 2021 ). Moreover, mutation of the lamin A/C gene D192G also led to weakened tunneling nanotubes (TNTs) and compromised cell–cell adhesion in neonatal rat ventricular fibroblasts, as shown by biomechanical studies using optical tweezers ( Lachaize et al, 2021 ).…”

Section: Single-molecule Studies Of Cardiovascular Diseasesmentioning

confidence: 99%

Application of optical tweezers in cardiovascular research: More than just a measuring tool

Yang

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

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Recent advances in the field of optical tweezer technology have shown intriguing potential for applications in cardiovascular medicine, bringing this laboratory nanomechanical instrument into the spotlight of translational medicine. This article summarizes cardiovascular system findings generated using optical tweezers, including not only rigorous nanomechanical measurements but also multifunctional manipulation of biologically active molecules such as myosin and actin, of cells such as red blood cells and cardiomyocytes, of subcellular organelles, and of microvessels in vivo. The implications of these findings in the diagnosis and treatment of diseases, as well as potential perspectives that could also benefit from this tool, are also discussed.

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“…Due to the subcellular scale and hard-to-reach location of the diagnostic structures inside cells, microrheology remains the most potential technique to probe these areas. Technologies incorporating microrheology may be developed to involve mechanical phenotype to explain the cause and progression or aid in the diagnosis of diseases ( Mukherjee et al, 2021 ). One can envision using microrheological techniques to map the structure and mechanical properties of the brain in real-time on a patient-by-patient basis, enabling researchers to monitor neural changes in response to various stimuli and disease progression.…”

Section: Summary and Future Prospectsmentioning

confidence: 99%

Passive and Active Microrheology for Biomedical Systems

Mao

Nielsen

Ali

2022

Front. Bioeng. Biotechnol.

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Microrheology encompasses a range of methods to measure the mechanical properties of soft materials. By characterizing the motion of embedded microscopic particles, microrheology extends the probing length scale and frequency range of conventional bulk rheology. Microrheology can be characterized into either passive or active methods based on the driving force exerted on probe particles. Tracer particles are driven by thermal energy in passive methods, applying minimal deformation to the assessed medium. In active techniques, particles are manipulated by an external force, most commonly produced through optical and magnetic fields. Small-scale rheology holds significant advantages over conventional bulk rheology, such as eliminating the need for large sample sizes, the ability to probe fragile materials non-destructively, and a wider probing frequency range. More importantly, some microrheological techniques can obtain spatiotemporal information of local microenvironments and accurately describe the heterogeneity of structurally complex fluids. Recently, there has been significant growth in using these minimally invasive techniques to investigate a wide range of biomedical systems both in vitro and in vivo. Here, we review the latest applications and advancements of microrheology in mammalian cells, tissues, and biofluids and discuss the current challenges and potential future advances on the horizon.

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Active microrheology using pulsed optical tweezers to probe viscoelasticity of lamin A

Abstract: Lamins are nucleoskeletal proteins of mammalian cells that stabilize the structure and maintain the rigidity of the nucleus. These type V intermediate filament proteins which are predominantly of A and...

Cited by 11 publications

References 49 publications

Experimental and theoretical studies of cross-stream migration of non-spherical particles in a quadratic flow of a viscoelastic fluid

Experimental and theoretical studies of cross-stream migration of non-spherical particles in a quadratic flow of a viscoelastic fluid

Application of optical tweezers in cardiovascular research: More than just a measuring tool

Passive and Active Microrheology for Biomedical Systems

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