Photosynthetic Carbon Assimilation 1978
DOI: 10.1007/978-1-4684-8106-8_15
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Active Site of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase

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Cited by 20 publications
(21 citation statements)
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“…cm-' ; Ogawa and Fujioka, 1980), aspartate transcarbamylase (5350 M-'.crn-'; Greenwell et al, 1973) and ribulose-1,5-bisphosphate carboxylase/oxygenase (4800 M-' . cm-' ; Paech and Tolbert, 1978) modified with pyridoxal 5'-phosphate/NaBH,. Since our value has been calculated from [I4C]CN-fixation, assuming that each cyanide ion trapped a pyridoxal 5'-phosphate molecule and corrected for nonspecific fixation of cyanide, an overestimation of its concentration (leading to an increase in F ) seems unlikely.…”
Section: Discussionmentioning
confidence: 99%
“…cm-' ; Ogawa and Fujioka, 1980), aspartate transcarbamylase (5350 M-'.crn-'; Greenwell et al, 1973) and ribulose-1,5-bisphosphate carboxylase/oxygenase (4800 M-' . cm-' ; Paech and Tolbert, 1978) modified with pyridoxal 5'-phosphate/NaBH,. Since our value has been calculated from [I4C]CN-fixation, assuming that each cyanide ion trapped a pyridoxal 5'-phosphate molecule and corrected for nonspecific fixation of cyanide, an overestimation of its concentration (leading to an increase in F ) seems unlikely.…”
Section: Discussionmentioning
confidence: 99%
“…Similar results were found with the Pxl-P inactivation of Rhs. rubrum RuBP carboxylase implicated in the interaction of PLP with spinach carboxylase [4]. Although activation by Pxl-P is most apparent when carboxylase activity is measured at low bicarbonate, Pxl-P does not appear to shorten the brief lag observed in the absence of Pxl-P ( fig.2).…”
Section: Resultsmentioning
confidence: 94%
“…In both cases, the chromophore at 330 nm [3], which is not reducible by sodium borohydride, was absent in these experiments performed with spinach carboxylase. Moreover, after modification with NaBT4 and Pxl-P and subsequent dissociation in sodium dodecyl sulfate, only large subunits were labeled under both inactivation [4] and activation conditions*.…”
Section: Resultsmentioning
confidence: 99%
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