Active transport of inducer in enzyme biosynthesis

Vieth, W. R.; Kaushik, Krishna; Venkasubramanian, K.

doi:10.1002/bit.260240617

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…In the present work, a relationship between p4 and the intracellular IPTG concentration is extracted from models of genetic control of protein synthesis and the lac ~p e r~n . '~~'~~~ Since the trc promotor is not subject to catabolite repression, the models of Vieth et al 36 and Vieth37 were simplified to a relationship between the rate of mRNA synthesis and the inducer concentration. This results in…”

Section: Alternative Modeling Approachmentioning

confidence: 99%

Dynamics of induced CAT expression in E. coli

Bentley

Davis

Kompala

1991

Biotech & Bioengineering

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The dynamics of chemically induced chloramphenicolaceyl-transferase (CAT) expression are determined in batch cultures of Escherichia coli DH5alphaF'IQ [pKK262-1]. This article is directed towards understanding the coupling of induced cloned-protein synthesis and reduced cell growth which are balanced in the optimal system. Experimental results indicate that the best inducer (IPTG) concentration is near 1.0 mM when added during midexponential growth. Lower concentrations cause only weak induction, whereas higher concentrations cause sufficiently strong induction that cell growth is suppressed. Induction at the onset of the stationary phase results in high expression but is accompanied by stimulated protease activity. Also, cell mass yield is adversely affected by enhanced protein synthesis. A structured metabolic model is shown to predict the responses of instantaneous growth rate and productivity which result from protein overexpression. This model can be employed to predict alternative reactor strategies and operating conditions necessary for the design of efficient bioprocess.

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Section: Alternative Modeling Approachmentioning

confidence: 99%

Dynamics of induced CAT expression in E. coli

Bentley

Davis

Kompala

1991

Biotech & Bioengineering

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“…Vieth et al (1982) have described lactose transport through the cell membrane by an equation of the following type: Active transport seems to be a common mode of transport of sugars into cells.…”

Section: Substrate Transport Into the Immobilized Cellmentioning

confidence: 99%

Chemical Engineering Analysis of Immobilized-Cell Systems

Venκatasubramanian

Karkare

Vieth

1983

Immobilized Microbial Cells

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Genetically structured models forlac promoter–operator function in the Escherichia coli chromosome and in multicopy plasmids: Lac operator function

Lee

Bailey

1984

Biotech & Bioengineering

115

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A mathematical model based on known molecular interactions has been formulated to describe quantitatively regulation of expression of the lactose (lac) operon in the Escherichia coli chromosome and in multicopy plasmids. This model is genetically structured such that a nucleotide sequence change affecting transcription initiation at the lac promoter-operator influences one or very few directly corresponding model parameters. The model simulates chromosomal lac operon function in good agreement with previous experimental measurements for many lacl and lacO mutant systems as well as for diploid cells which carry F'lac episomes. Simulation results clearly show the loss of cloned lac operator regulation as the plasmid copy number increases, in agreement with experimental trends. The importance of this class of models in designing DNAs, organisms, and reactors for precise regulation of cloned gene expression is discussed.

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Active transport of inducer in enzyme biosynthesis

Cited by 8 publications

References 9 publications

Dynamics of induced CAT expression in E. coli

Dynamics of induced CAT expression in E. coli

Chemical Engineering Analysis of Immobilized-Cell Systems

Genetically structured models forlac promoter–operator function in the Escherichia coli chromosome and in multicopy plasmids: Lac operator function

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