Active transport of oil droplets along oriented microtubules by kinesin molecular motors

Bottier, Céline; Fattaccioli, Jacques; Tarhan, Mehmet C.; Yokokawa, Ryuji; Morin, Fabrice O.; Kim, Beomjoon; Collard, Dominique; Fujita, Hiroyuki

doi:10.1039/b822519b

Cited by 28 publications

(27 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By exploiting the well-characterized ligand-receptor pair biotin-streptavidin, EDs were immobilized on glass surfaces (9) and actively transported along microtubules (10). In the context of self-assembly, both polymer-mediated (11-13) and biotin-streptavidin-mediated aggregation of EDs was shown (14).…”

mentioning

confidence: 99%

Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets

Hadorn

Boenzli

Sørensen

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Higher-order structures that originate from the specific and reversible DNA-directed self-assembly of microscopic building blocks hold great promise for future technologies. Here, we functionalized biotinylated soft colloid oil-in-water emulsion droplets with biotinylated single-stranded DNA oligonucleotides using streptavidin as an intermediary linker. We show the components of this modular linking system to be stable and to induce sequencespecific aggregation of binary mixtures of emulsion droplets. Three length scales were thereby involved: nanoscale DNA base pairing linking microscopic building blocks resulted in macroscopic aggregates visible to the naked eye. The aggregation process was reversible by changing the temperature and electrolyte concentration and by the addition of competing oligonucleotides. The system was reset and reused by subsequent refunctionalization of the emulsion droplets. DNA-directed self-assembly of oil-in-water emulsion droplets, therefore, offers a solid basis for programmable and recyclable soft materials that undergo structural rearrangements on demand and that range in application from information technology to medicine.C omplex higher-order structures that spontaneously arise from the specific and reversible self-assembly of simple building blocks hold great promise to become the foundation of tomorrow's technology (1). In the context of DNA-directed self-assembly of hard colloids and artificial vesicles, linear DNA oligonucleotides were shown to be adhesive elements that allowed the self-assembly process not only to be specific but also to be reversible by a variety of external stimuli such as temperature (2-4), changes in the electrolyte concentration (4, 5), addition of competing oligonucleotides (6), and exploitation of the secondary structure of DNA oligonucleotides (7).Oil-in-water (o/w) emulsion droplets (EDs) offer a robust, configurable, and recyclable soft-colloid system with interesting properties for the fabrication of new delivery systems and advanced synthetic materials with adaptable properties (8). By exploiting the well-characterized ligand-receptor pair biotin-streptavidin, EDs were immobilized on glass surfaces (9) and actively transported along microtubules (10). In the context of self-assembly, both polymer-mediated (11-13) and biotin-streptavidin-mediated aggregation of EDs was shown (14). Despite the advantages a combination of EDs as building blocks and DNA oligonucleotides as linking agent offers, a protocol for DNA-directed self-assembly of EDs is currently missing.To close this gap, here we prepared an o/w emulsion composed of heavier-than-water diethyl phthalate (DEP) dispersed in an aqueous medium, stabilized by phospholipids, and functionalized with single-stranded DNA (ssDNA) oligonucleotides. Palmitoyloleoyl phosphatidylcholine (POPC) and distearoylphosphatidyl ethanolamine (DSPE) in a 9:1 molar ratio were used to stabilize the EDs. The DSPE was grafted with flexible poly(ethylene glycol) (PEG) tethers with a molecular weight of 2,000 (DSPE-PEG...

show abstract

mentioning

confidence: 99%

Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets

Hadorn

Boenzli

Sørensen

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…However, liquid manipulation, that is, unidirectional flow, is still necessary for the orientation of MTs in the channel. MTs can be oriented inside relatively large, "surrogate" PDMS channels [17] before the final microfluidic channel system with smaller channel dimensions is applied on the substrate. However, the handling of a PDMS slab with sub-micrometer features presents its own challenges.…”

Section: Molecular Sorting and Transportmentioning

confidence: 99%

“…In bead assay, MTs are immobilized on a glass surface and kinesin molecules perform unidirectional motion along the MTs. [14] The tail of the kinesin molecule can be used to attach cargo, such as microspheres, [15] quantum dots, [16] oil droplets, [17] and micromachined structures. [18][19][20] Another issue with handling extremely small amounts of molecules is ensuring a highly selective capturing mechanism.…”

Section: Introductionmentioning

confidence: 99%

Specific Transport of Target Molecules by Motor Proteins in Microfluidic Channels

et al. 2013

Self Cite

View full text Add to dashboard Cite

Direct transport powered by motor proteins can alleviate the challenges presented by miniaturization of microfluidic systems. There have been several recent attempts to build motor-protein-driven transport systems based on simple capturing or transport mechanisms. However, to achieve a multifunctional device for practical applications, a more complex sorting/transport system should be realized. Herein, the proof of concept of a sorting device employing selective capture of distinct target molecules and transport of the sorted molecules to different predefined directions is presented. By combining the bottom-up functionality of biological systems with the top-down handling capabilities of micro-electromechanical systems technology, highly selective molecular recognition and motor-protein-based transport is integrated in a microfluidic channel network.

show abstract

“…Bottier et al used a hybrid platform in which kinesin motors actively carry oil-in-water droplets along microtubules [72]. The transport of droplets along organic tracks has recently attracted attention.…”

Section: Droplet-based Micro and Nanotechnologymentioning

confidence: 99%

Droplet microfluidics: A tool for biology, chemistry and nanotechnology

Mashaghi

Abbaspourrad

Weitz

et al. 2016

TrAC Trends in Analytical Chemistry

322

188

View full text Add to dashboard Cite

The ability to perform laboratory operations on small scales using miniaturized devices provides numerous benefits, including reduced quantities of reagents and waste as well as increased portability and controllability of assays. These operations can involve reaction components in the solution phase and as a result, their miniaturization can be accomplished through microfluidic approaches. One such approach, droplet microfluidics, provides a high-throughput platform for a wide range of assays and approaches in chemistry, biology and nanotechnology. We highlight recent advances in the application of droplet microfluidics in chipbased technologies, such as single-cell analysis tools, small-scale cell cultures, in-droplet chemical synthesis, high-throughput drug screening, and nanodevice fabrication.

show abstract

Active transport of oil droplets along oriented microtubules by kinesin molecular motors

Cited by 28 publications

References 36 publications

Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets

Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets

Specific Transport of Target Molecules by Motor Proteins in Microfluidic Channels

Droplet microfluidics: A tool for biology, chemistry and nanotechnology

Contact Info

Product

Resources

About