Activit�s de quelques enzymes associ�s � la conidiogen�se du Neurospora crassa

Combépine, G.; Turian, G.

doi:10.1007/bf00411013

Cited by 13 publications

(6 citation statements)

References 21 publications

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“…Under the latter conditions, Stine (38) reported fluffy to have NADase levels many times that of the wild-type strain. However, in our experiments, only low levels of NADase could be detected in the fluffy strain when grown under these conditions, which is consistent with the hypothesis that NADase is associated with conidiogenesis (5,39,47,51). The factor(s) responsible for the differences between the results reported here and those of Stine (38) have not been identified.…”

Section: Vol 120 1974supporting

confidence: 88%

Rhythms of Enzyme Activity Associated with Circadian Conidiation in Neurospora crassa

Hochberg

Sargent

1974

J Bacteriol

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The mycelial growth front of the band strain of Neurospora grown on a solid surface exhibits a circadian rhythm of conidiation. Enzyme assays on extracts from that mycelium have shown that the activities of 6 of 13 enzymes (nicotinamide adenine dinucleotide nucleosidase, isocitrate lyase, citrate synthase, glyceraldehydephosphate dehydrogenase, phosphogluconate dehydrogenase, and glucose-6-phosphate dehydrogenase) and soluble-protein content oscillate with the visible morphological change. The rhythmic enzymes associated with the Krebs and glyoxylate cycles are more active during conidiogenesis, whereas the activities of the rhythmic enzymes of glycolysis and the hexose monophosphate shunt are reduced during that phase. The absence of enzyme oscillations in wild-type and fluffy strains which do not form conidia under the conditions employed suggests that the enzyme fluctuations are associated with conidiogenesis itself. Oscillations of enzyme activity as a function of time are restricted to the growth front. A permanent record of rhythmicity associated with conidial and nonconidial regions does, however, exist in the mycelial mat behind the growth front. The activities of three enzymes (nicotinamide adenine dinucleotide nucleosidase, glucose-6-phosphate dehydrogenase, and phosphogluconate dehydrogenase) are not directly influenced by CO2 concentration, but are correlated with the prescence or absence of conidiation which is controlled by CO2 concentration. In contrast, citrate synthase and malate dehydrogenase activities are correlated with changes in CO2 concentration.

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Section: Vol 120 1974supporting

confidence: 88%

Rhythms of Enzyme Activity Associated with Circadian Conidiation in Neurospora crassa

Hochberg

Sargent

1974

J Bacteriol

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“…laminarinase, invertase, phospholipase, trehalase and NADase from Neurospora crassa (Mahadevan & Menon, 1968;Metzenberg, 1963;Chakravarti et al, 1981;Hill & Sussman, 1964;Combepine & Turian, 1970) P-glucosidase from Aspergillus oryzae (Horikoshi & Ikeda, endo-1,4-P-glucanase, (0) and purified endo-1 ,4-P-glucanase ( 0 ) at 5 pg ml-l. The enzymes were kept at the indicated temperature for 60 min, and the recovery of original activity is indicated.…”

Section: Eject O J P-octylglucoside Treatment On the Ability Of T Rementioning

confidence: 99%

Properties of a Conidial-bound Cellulase Enzyme System from Trichoderma reesei

Kubicek¹,

Mühlbauer²,

Klotz³

et al. 1988

Microbiology

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“…NADase [NAD(P) glycohydrolase; EC 3.2.2.5] is uniquely associated with aerial hyphae and conidia when grown on normal media (31,193,195,215,245). However, this enzyme can be induced in mycelia by zinc starvation (52) and thus is not absolutely unique to conidia.…”

Section: Exocellular Enzymesmentioning

confidence: 99%

Section: Macromoleculesmentioning

confidence: 99%

“…Different isozyme patterns of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) have been detected in conidia and in vegetative mycelia (31). Conidia had three electrophoretically distinguishable glucose-6-phosphate dehydrogenase activities, whereas only two were detected in mycelia harvested after 3 days of growth in sucrose medium (31). However, three distinguishable activities have been found in mycelia grown in glucose minimal medium (174,175) and in mycelia from cultures grown for 3 days in acetate medium (31).…”

Section: Macromoleculesmentioning

confidence: 99%

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