Biochemical genetics of Neurospora crassa conidial germination.

Schmit, Joseph C.; Brody, Stuart

doi:10.1128/mmbr.40.1.1-41.1976

Cited by 100 publications

(71 citation statements)

References 156 publications

(328 reference statements)

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“…A`candidate-gene' approach has been very successful in isolating mutants defective in late germination and appressorium formation in M. grisea [12], U. maydis [13] and Colletotrichum lagenarium [14]. Inhibitors were widely used in many early studies (for example, see [15]), but interpretation of those results is complicated, because most inhibitors are not truly speci¢c.…”

Section: Approaches Used To Identify Genes and Proteins Involved In Cmentioning

confidence: 99%

“…Magnaporthe, Colletotrichum, Ustilago), the hyphal tip swells to form the appressorium. At the same time, numerous metabolic activities, including respiration, RNA and protein synthesis, trehalose breakdown as well as di¡erences in the composition of the cell wall can be detected [15,18].…”

Section: A Short Description Of Conidial Germinationmentioning

confidence: 99%

“…Conidial germination in most ¢lamentous fungi requires the presence of low-molecular-mass nutrients such as sugars, amino acids and inorganic salts [19]. In N. crassa, germination occurs in the presence of salt and a carbon source alone [15], whereas in A. nidulans, the presence of glucose alone is su¤cient to induce germination [8]. Considerable research has been carried out on the molecular basis of the glucose-sensing pathways in S. cerevisiae [20], and this, although not dealing directly with conidial germination, may provide interesting possible parallels between the two processes.…”

Section: Triggers Of Conidial Germinationmentioning

confidence: 99%

“…This has led to the hypothesis that glutamic acid reduction generates NAD(P)H which is used to generate reduced glutathione. In turn, glutathione activates the proteins necessary for germination by breaking protein disul¢de (Cys-S^S-Cys) bonds into Cys-SH-free residues, leading to their activation ( [51] and reviewed in [15]). The validity of this model has not been pursued experimentally, nor does it explain the necessity for the other elements of signaling discussed in this review.…”

Section: The Protein Disul¢de Model In N Crassamentioning

confidence: 99%

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The molecular mechanisms of conidial germination

Osherov

2001

FEMS Microbiology Letters

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The asexual spore, or conidium, is critical in the life cycle of many fungi because it is the primary means for dispersion and serves as a`safe house' for the fungal genome in adverse environmental conditions. This review discusses the physiological process of germination, conidial adhesion and initiation of protein synthesis and also the regulatory pathways used to activate conidial germination. These include Ca 2 / calmodulin-mediated signaling, the cyclic AMP/protein kinase A and the ras/mitogen-activated protein kinase pathways. Insights into the process of conidial germination will increase our understanding of the mechanisms of dormancy and sensing of environmental stimuli, and permit identification of novel therapeutic targets for the treatment of spore-borne fungal infections in plants and animals. ß

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Section: Approaches Used To Identify Genes and Proteins Involved In Cmentioning

confidence: 99%

Section: A Short Description Of Conidial Germinationmentioning

confidence: 99%

Section: Triggers Of Conidial Germinationmentioning

confidence: 99%

Section: The Protein Disul¢de Model In N Crassamentioning

confidence: 99%

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The molecular mechanisms of conidial germination

Osherov

2001

FEMS Microbiology Letters

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“…Asexual life cycle in N . crassa is very simple being constituted by only three different cell types : vegetative hyphae, aerial hyphae and conidia [6]. .…”

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confidence: 99%

Purification and Some Properties of NAD‐Glycohydrolase from Conidia of Neurospora crassa

Menegus¹,

Pace

1981

European Journal of Biochemistry

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NAD‐glycohydrolase from conidia of Neurospora crassa was purified by affinity chromatography, using 4‐methylnicotinamide adenine dinucleotide as ligand immobilized onto Sepharose through a hydrophilic spacer arm. The pure enzyme is a glycoprotein with an isoelectric point of 5.5 and a molecular weight of 33000 as determined by sodium dodecylsulphate gel electrophoresis. The specific activity is the highest so far found for NAD‐glycohydrolases and in various aspects the enzyme is different from that isolated from mycelia ofN. crussa grown in a ‘zinc‐deficient’ medium.

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Genetic control of fungal differentiation: The three sporulation pathways of Neurospora crassa

1993

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Sporulation in the mold Neurospora crassa can proceed along three very different pathways, leading to the production of three types of spores. Two asexual sporulation pathways that lead to the formation of macroconidia and microconidia involve budding from hyphae by two different mechanisms. A much more complex sexual reproductive pathway involves the formation of a fruiting body called a perithecium, in which meiosis takes place and ascospores are formed in sac-like cells called asci. Numerous mutations exist that affect these developmental pathways and genes have been isolated that are expressed preferentially during sporulation. The Neurospora sporulation pathways offer a simple system with which to study mechanisms and regulation of development that are usually obscured by complex cell-cell interactions involved in animal and plant development.

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Biochemical genetics of Neurospora crassa conidial germination.

Cited by 100 publications

References 156 publications

The molecular mechanisms of conidial germination

The molecular mechanisms of conidial germination

Purification and Some Properties of NAD‐Glycohydrolase from Conidia of Neurospora crassa

Genetic control of fungal differentiation: The three sporulation pathways of Neurospora crassa

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