Activities of Antioxidant Enzymes during Senescence of Prunus Armeniaca Leaves

Scebba, Francesca; Sebastiani, L.; Vitagliano, C.

doi:10.1023/a:1017962102950

Cited by 91 publications

(33 citation statements)

References 20 publications

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“…Peroxidase (EC 1.11.1.7) activity was determined at 25°C according to Scebba et al (2001) . The reaction mixture contained 2.25 mM guaiacol, 11 mM H 2 O 2 in 0.1 M phosphate buffer (pH 6.0), and 100 μl of the enzyme extract in the total volume of 2 ml.…”

Section: Methodsmentioning

confidence: 99%

Exogenous Melatonin Improves Antioxidant Defense in Cucumber Seeds (Cucumis sativus L.) Germinated under Chilling Stress

2016

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The relationship between exogenous melatonin applied into cucumber seeds during osmopriming and modifications of their antioxidant defense was studied. Accumulation of hydrogen peroxide, antioxidant enzyme activities and glutathione pool were investigated in embryonic axes isolated from the control, osmoprimed, and osmoprimed with melatonin seeds. Germinating cucumber seeds are very sensitive to chilling. Temperature 10°C causes oxidative stress in young seedlings. Seed pre-treatment with melatonin seemed to limit H2O2 accumulation during germination under optimal condition as well as during chilling stress and recovery period. Melatonin affected superoxide dismutase (SOD) activity and its isoforms during stress and recovery period but did not influence CAT and POX activities. Thus it is possible that in cucumber this indoleamine could act mostly as a direct H2O2 scavenger, but superoxide anion combat via SOD stimulation. The GSH/GSSG ratio is considered as an indirect determinant of oxidative stress. When the cells are exposed to oxidative stress GSSG is accumulated and the ratio of GSH to GSSG decreases. In our research pre-sowing melatonin application into the cucumber seeds caused high beneficial value of GSH/GSSG ratio that could be helpful for stress countering. Glutathione reductase (GSSG-R) activity in the axes isolated from these seeds was two fold higher than in those isolated from the control and from the osmoprimed without melatonin ones. Additional isoforms of GSSG-R in melatonin treated seeds were also observed. It explains high and effective GSH pool restoration in the seeds pre-treated with melatonin. We confirmed that melatonin could protect cucumber seeds and young seedlings against oxidative stress directly and indirectly detoxifying ROS, thereby plants grown better even in harmful environmental conditions. This work is the first that investigated on plant in vivo model and documented melatonin influence on redox state during seed germination. This way we try to fill lack of information about melatonin-regulated pathways involved in antioxidant strategy of plant defense.

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Section: Methodsmentioning

confidence: 99%

Exogenous Melatonin Improves Antioxidant Defense in Cucumber Seeds (Cucumis sativus L.) Germinated under Chilling Stress

2016

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“…Superoxide dismutase (SOD) activity was assayed by the nitroblue tetrazolium (NBT) method [41] based on photochemical reduction of nitro blue tetrazolium (NBT) as monitored at 560 nm. Activities of peroxidase (POD) and catalase (CAT) were assayed using the method of Scebba et al [42]. POD was assayed at 470 nm by using hydrogen peroxide and guaiacol as the substrates.…”

Section: Assays Of Antioxidative Enzyme Activitiesmentioning

confidence: 99%

Melatonin Alleviates Drought Stress by a Non-Enzymatic and Enzymatic Antioxidative System in Kiwifruit Seedlings

Xia

et al. 2020

IJMS

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Although melatonin was affirmed to alleviate drought stress in various plant species, the mechanism in kiwifruit remains to be elucidated. In this study, the transcriptomes of kiwifruit leaves under control (CK), DR (drought stress), and MTDR (drought plus melatonin) treatments were evaluated. After comparisons of the gene expression between DR and MTDR, the differentially expressed genes (DEGs) were screened. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated three significant pathways, which were mainly involved in the glutathione metabolism, ascorbate and aldarate metabolism, and carotenoid metabolism. Therefore, the content and metabolic gene expression level of ascorbic acid (AsA), glutathione, and carotenoid were higher in the MTDR treatment than that in others. Furthermore, the activity and mRNA expression level of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were also promoted in the MTDR group. Combined with these results of important secondary metabolites and protective enzymes measured in the seedlings in different treatments, it could be concluded that exogenous melatonin induced the ascorbic acid-glutathione (AsA-GSH) cycle, carotenoid biosynthesis, and protective enzyme system to improve seedling growth. Our results contribute to the development of a practical method for kiwifruit against drought stress.

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“…Superoxide dismutase (SOD; EC 1.15.1.1) activity was measured based on its ability to inhibit the photochemical reduction of Nitroblue tetrazolium 39 . Peroxidase (POD; EC 1.11.1.7) activity was measured at 25 °C by monitoring the increase in absorbance at 470 nm 40 . Catalase (CAT; EC 1.11.1.6) activity was measured at 25 °C by the absorbance decrease at 240 nm due to the H 2 O 2 decomposition 41 .…”

Section: Methodsmentioning

confidence: 99%

Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

Zhang

Wang

et al. 2017

Sci Rep

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Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused “dwarf” phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the CesA gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface. The proline and soluble sugar content increased contrarily. Under the salt stress, the T3 of RNAi plants showed significant higher resistance. The expression levels of some salt tolerance related genes (BoiProH, BoiPIP2;2, BoiPIP2;3) were significantly changed in T3 of RNAi plants. The results showed that the hairpin structure of CesA specific fragment inhibited the endogenous gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis. Moreover, modulation cellulose synthesis probably was an important influencing factor in polysaccharide metabolism and adaptations of plants to stresses. This will provide technological possibilities for the further study of modulation of the cellulose content of crops.

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Activities of Antioxidant Enzymes during Senescence of Prunus Armeniaca Leaves

Cited by 91 publications

References 20 publications

Exogenous Melatonin Improves Antioxidant Defense in Cucumber Seeds (Cucumis sativus L.) Germinated under Chilling Stress

Exogenous Melatonin Improves Antioxidant Defense in Cucumber Seeds (Cucumis sativus L.) Germinated under Chilling Stress

Melatonin Alleviates Drought Stress by a Non-Enzymatic and Enzymatic Antioxidative System in Kiwifruit Seedlings

Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

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