Activities of carbon and nitrogen metabolism enzymes during germinating sorghum seeds and early seedlings growth

Mrid, Reda Ben; Omari, Redouane El; Bouargalne, Youssef; Mourabit, Nourdin El; Nhiri, Mohamed

doi:10.1556/0806.45.2017.044

Cited by 12 publications

(4 citation statements)

References 35 publications

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“…Furthermore, the treatment with Comp-4 at 12.5 μM led to the highest Gly I activity, and the treatment with Cur, Ros-A at 25 μM, and Comp-4 at 25 μM were responsible for the highest Gly II activity. The increase in the enzyme activities of the glyoxalase system is correlated with the increase in GR activity, for which the pool of GSH generated is used as a cofactor for the Gly I enzyme (Ben Mrid et al, 2018); what is more, under oxidative stress or aging conditions, the activity of Gly I was reported to decrease, leading to an increase in glycation reaction and therefore, tissue injury. On the other hand, overexpression of Gly I in endothelial cells under high concentration of glucose inhibited the formation of the advanced glycation end products (AGEs), indicating the importance of this enzyme in detoxifying cells from MG, the primary precursor implicated in the formation of AGEs (Suh et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Synergistic antioxidant effects of natural compounds on H2O2-induced cytotoxicity of human monocytes

et al. 2022

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Natural compounds are endowed with a broad spectrum of biological activities, including protection against Toxins. Most of them are known for their antioxidant and radical scavenging activities. However, the synergistic combination of these natural molecules is not well studied. Therefore, the present study aims first to investigate the effect of four potent natural molecules [rosmarinic acid (Ros-A), ellagic acid (Ella-A), curcumin (Cur), and syringic acid (Syr-A)] on H2O2 -induced cell cytotoxicity and oxidative stress on the human monocytes (THP-1) and then to evaluate their combined action effect. Optimal combinations of these molecules were predicted using an augmented mixture design approach. In the first, as preliminary antioxidant activities screening, two in vitro assays were adopted to assess the single radicals scavenging activity of these natural compounds, DPPH• and ABTS• + tests. Based on the results obtained, the multitude of optimal formulas proposed by the mixture design study led to choosing four potent compositions (comp) in addition to ellagic acid, proposed as the most efficient when applied alone. The different molecules and mixtures were used to assess their cytoprotective effect on THP-1 cells in the presence and absence of H2O2. The most potent Comp-4, as well as the molecules forming this mixture, were exploited in a second experiment, aiming to understand the effect on oxidative stress via antioxidant enzyme activities analysis in the H2O2-induced oxidative stress in the THP-1 cell line. Interestingly, the natural molecules used for THP-1 cells treatment exhibited a significant increase in the antioxidant defense and glyoxalase system as well as suppression of ROS generation evaluated as MDA content. These results indicate that the natural compounds tested here, especially the synergistic effect of Cur and Ros-A (Comp-4), could serve as cytoprotective and immunostimulant agents against H2O2-induced cytotoxicity THP-1 cells, which makes them interesting for further investigations on the molecular mechanisms in preclinical animal models.

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Section: Discussionmentioning

confidence: 99%

Synergistic antioxidant effects of natural compounds on H2O2-induced cytotoxicity of human monocytes

et al. 2022

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“…The increase in the NADP+-ICDH could also be due to a higher demand for carbon skeletons in the forms of 2-oxoglutarate as proposed by [58]. The carbon generated through this reaction may be used for the process of biosynthesis of amino acids [59].…”

Section: Effect Of Ure On Plant Growth and Oxidative Stress Metabolism Of Wheat Plantsmentioning

confidence: 99%

The effect of foliar application of Ulva rigida extract on the growth and biochemical parameters of wheat plants

Latique

Mrid

Kabach³

et al. 2021

E3S Web Conf.

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Many research studies have shown the importance of seaweed extract in agriculture. Marine macroalgae are rich in diverse compounds like lipids, proteins, carbohydrates, phytohormones, amino acids, osmoprotectants and minerals. In this study, we have tried to characterize Moroccan macroalgae species Ulva rigida collected from the coastal area of Akhfenir in order to evaluate their potential to improve growth plant. The proximate composition, mineral element (Ca, K, Mg, Na and Cl), pigments, amino acid, proteins, lipids, polyphenol contents were determined in liquid extract obtained from green seaweed (SWE) Ulva rigida. Results show that the application of SWE at different concentrations (0,12.5, 25, 50%) significantly enhanced growth parameters, especially with 25% of seaweed liquid extract. Therefore, algal treatment by foliar application is proved to be an effective technique to improve the growth of wheat plants. This study provides important information on the characterization, identification and utilization of seaweed resources for agriculture as biostimulants.

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“…The total soluble protein content of the enzyme extracts was determined using the Bradford method [32], with the protein standard for the calibration curve being bovine serum albumin (BSA).…”

Section: Protein Determinationmentioning

confidence: 99%

Saccorhiza polyschides Extract as Biostimulant for Reducing Salt Stress Effect in Common Bean Crops

Nhhala,

Latique,

Kchikich

et al. 2024

Preprint

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Salinity stress emerges as an important menace to global food security, particularly affecting susceptible crops such as common bean plants (Phaseolus vulgaris L.). The disruption of ion balances caused by salt stress withing plants cells reduces water and nutrient absorption, and hampers growth and productivity. Sacchorhiza polyschides, a brown macroalga rich in essential elements, and macrominerals, has drawn attention as a natural biostimulant, being a potential remedy to bolster crop resilience against salt stress. This study delves into the effect of water extracts of Sacchorhiza polyschides (SPE) across varying concentrations (1%, 2%, and 5%) in common bean plants under salt stress (68.4 mM NaCl) conditions. Two different methods of application were used: by spraying the aerial part of the plant or by irrigation together with the watering solution. The effects of both systems of application were studied on the aerial parts of the plants. SPE exhibited significant enhancements in morphological parameters, chlorophyll content, carbon-nitrogen activities, and antioxidant enzymatic system activities, alongside increasing osmolyte accumulation, compared to salt-stressed plants without biostimulation. Moreover, SPE reduced reactive oxygen species (ROS) levels caused by salt stress, mitigating lipid peroxidation. The concentrations of 2% and 5% SPE applied by spraying showed the most significant results in most of the parameters analyzed. By shedding light on the underlying science behind this biostimulant effect, this research underscores the potential of SPE to enhance crop resilience and augment food production in salt-challenged environments.

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Activities of carbon and nitrogen metabolism enzymes during germinating sorghum seeds and early seedlings growth

Cited by 12 publications

References 35 publications

Synergistic antioxidant effects of natural compounds on H2O2-induced cytotoxicity of human monocytes

Synergistic antioxidant effects of natural compounds on H2O2-induced cytotoxicity of human monocytes

The effect of foliar application of Ulva rigida extract on the growth and biochemical parameters of wheat plants

Saccorhiza polyschides Extract as Biostimulant for Reducing Salt Stress Effect in Common Bean Crops

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