Activities of Hydrogen Peroxide-Scavenging Enzymes in Germinating Wheat Seeds

Morphological and Biochemical Answer of the Wheat Seeds at Treatment with 2,4-Dinitrophenol and Potassium Iodate

“…Indeed, at higher concentrations than 3x10 -3 M, DNP completely inhibited seed germination. Our data are in agreement with other authors (Cakmak et al 1993) that suggest that the enzyme activity of POX, CAT, SOD and GPX (glutathione peroxidase) increases during the germination of seeds, and, especially, during the emergence of the radicles (Kang and Saltveit 2002). Indeed, after 24-48 hours from the imbibition, the increase in enzyme activities is much higher in the embryo than in the endosperm, whereas the SOD activity increases less after 72 hours in comparison with other enzymes (Dučić et al 2003/4;Prodanović et al 2007).…”

Oxidative stress induced expression of monodehydroascorbate reductase gene in Eleusine coracana

“…Under in vivo stress conditions, the seeds were germinated and the seedlings were grown in MS medium supplemented with NaCl, which enhanced the stress exposure of the seedlings, whereas in poly-house grown plants (in vitro stress treatment) the seeds were germinated and the seedlings were first grown under optimal conditions and only later when they have grown to a particular stage, they were subjected to stress. It is well-known that the plants are more prone to stress induced injury at the time of seed germination, as compared to the vegetative growth stages, wherein they develop the capability to combat the imposed stress (Cakmak et al 1993). Therefore the level of mdar gene expression as well as the protein activity were subject to severe stress consequences in the in vivo stress conditions as compared to the in vitro stress conditions.…”

Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

“…The activity was calculated from the change in absorbance at 265 nm for 1 min using extinction co-efficient of 14 mM −1 cm −1 . GR (EC: 1.6.4.2) activity was measured by the method of Cakmak et al (1993). The reaction mixture contained 0.1 M K-phosphate buffer (pH 7.8), 1 mM EDTA, 1 mM GSSG, 0.2 mM NADPH, and enzyme solution in a final volume of 1 ml.…”