Activities of respiratory chain complexes in isolated platelets in females with anorexia nervosa

Böhm, M.; Papežová, Hana; Hansı́ková, Hana; Wenchich, László; Zeman, Jiřı́

doi:10.1002/eat.20403

Cited by 15 publications

(11 citation statements)

References 29 publications

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“…The activities of respiratory chain Complexes I and II in isolated platelets were signifi cantly higher in females with anorexia nervosa in comparison with control group. No differences were found in the activities of Complexes IV, I+III, and citrate synthase (39).…”

Section: Discussionmentioning

confidence: 74%

“…The study of physiological and pathological mitochondrial function is essential for the diagnostics and targeting therapy of mitochondrial diseases. It was shown that circulating platelets can act as sensors of the infl ammatory and metabolic stresses associated with various chronic diseases, as cardiovascular disease, neurodegenerative diseases and diabetes (39). The respirometry of human platelets allows dynamic monitoring of mitochondrial function.…”

Section: Discussionmentioning

confidence: 99%

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Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method

Gvozdjáková¹,

Sumbalová²,

Kucharská³

et al. 2019

BLL

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OBJECTIVES: To test the hypothesis if mitochondrial bioenergetic function analyzed in circulating platelets may represent peripheral signature of mitochondrial dysfunction in nephropathy associated to noncommunicable human diseases such as cardiovascular diseases, diabetes and with statins treatment. METHODS: High-resolution respirometry was used for analysis of mitochondrial bioenergetics in human platelets isolated from peripheral blood. This method is less-invasively compared to skeletal muscle biopsy. Patients with nephropathies and in combination with non-communicable diseases were included in the study. RESULTS: This pilot study showed platelet mitochondrial bioenergy dysfunction in patients with nephropathies and non-communicable diseases. Positive effect of treatment with 10 mg atorvastatin on platelet mitochondrial respiratory chain Complex I-linked respiration and ATP production in patients with nephropathies, diabetes and 80 mg atorvastatin in patient with nephropathy and dialysis was found. Positive effect of 80 mg fl uvastatin treatment, and negative effect of thrombocytopenia and renal transplantation on platelet mitochondrial bioenergy was determined. CONCLUSION: High-resolution respirometry allowed detection of small changes in platelet mitochondrial function. This method could be used as a sensitive bioenergetic test of mitochondrial function for diagnosis and monitoring the therapy in patients with nephropathy (Tab. 1, Fig. 3, Ref. 39). Text in PDF www.elis.sk.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method

Gvozdjáková¹,

Sumbalová²,

Kucharská³

et al. 2019

BLL

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“…Studies examining mitochondrial function are typically measured in permeabilized skeletal muscle fibers. However, platelet mitochondria have been established as well suited for ex vivo analysis of cellular respiration (Sjövall et al 2010, Hroudová et al 2013) and the activity of respiratory chain complexes (Böhm et al 2007). Platelets are an easily obtainable source of viable mitochondria and disturbances in the function of platelet mitochondria have been demonstrated in aging (Merlo Pich et al 1996, Lenaz et al 2000, Xu et al 2007) and in variety of diseases, including neurodegenerative disorders (Krige et al 1992, Hauptmann et al 2006, Shi et al 2008, Hroudová et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Statin-Induced Changes in Mitochondrial Respiration in Blood Platelets in Rats and Human With Dyslipidemia

Vevera

Fišar

Nekovarova³

et al. 2016

Physiol Res

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3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) are widely used drugs for lowering blood lipid levels and preventing cardiovascular diseases. However, statins can have serious adverse effects, which may be related to development of mitochondrial dysfunctions. The aim of study was to demonstrate the in vivo effect of high and therapeutic doses of statins on mitochondrial respiration in blood platelets. Model approach was used in the study. Simvastatin was administered to rats at a high dose for 4 weeks. Humans were treated with therapeutic doses of rosuvastatin or atorvastatin for 6 weeks.Platelet mitochondrial respiration was measured using highresolution respirometry. In rats, a significantly lower physiological respiratory rate was found in intact platelets of simvastatintreated rats compared to controls. In humans, no significant changes in mitochondrial respiration were detected in intact platelets; however, decreased complex I-linked respiration was observed after statin treatment in permeabilized platelets. We propose that the small in vivo effect of statins on platelet energy metabolism can be attributed to drug effects on complex I of the electron transport system. Both intact and permeabilized platelets can be used as a readily available biological model to study changes in cellular energy metabolism in patients treated with statins.

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“…The lack of energy supply in the patients with anorexia nervosa may be compensated by a higher efficiency of ATP production. In adult females with anorexia nervosa, Böhm and coworkers [28] suggest a higher efficiency of the respiratory complexes I and II in isolated Table 3 Coenzyme Q10 in juveniles (n 5 16) with anorexia nervosa at the time points of admission to and discharge from the children's hospital platelets, but the authors suggest normal activity of complexes III and IV which probably contests the facility of an increased total capacity in oxidative phosphorylation in patients suffering from anorexia nervosa. An inhibition of mitochondrial energy metabolism is supposed for inborn errors of metabolism like methyl malonyl aciduria (MMA).…”

Section: Discussionmentioning

confidence: 95%

Coenzyme Q10 concentration in plasma and blood cells of juvenile patients hospitalized for anorexia nervosa

et al. 2012

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The antioxidant status of coenzyme Q10 (CoQ10) was investigated in plasma, erythrocytes, and platelets of juvenile patients with anorexia nervosa. Blood for analysis of the CoQ10 status was taken from 16 juvenile patients suffering from anorexia nervosa (restricting form) at the time point of admission to the hospital and at discharge after about 12 weeks. Plasma and blood cells isolated by a density gradient were stored at -84 °C until analysis. CoQ10 concentration and redox status were measured by high pressure liquid chromatography with electrochemical detection and internal standardization. The improvement of physical health during the hospital refeeding process was followed up by the body mass index (BMI). The antioxidant status of plasma CoQ10 in juvenile patients suffering from anorexia nervosa indicated no abnormalities in comparison to healthy controls. However, the decreased concentration of CoQ10 observed in platelets at the time point of hospital admission may represent mitochondrial CoQ10 depletion. This initial deficit improved during the hospital refeeding process. The platelet CoQ10 concentration showed a positive correlation to the BMI of the patients.

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Activities of respiratory chain complexes in isolated platelets in females with anorexia nervosa

Cited by 15 publications

References 29 publications

Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method

Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method

Statin-Induced Changes in Mitochondrial Respiration in Blood Platelets in Rats and Human With Dyslipidemia

Coenzyme Q10 concentration in plasma and blood cells of juvenile patients hospitalized for anorexia nervosa

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