Activities of Some Medicinal Plants on the Proliferation and Invasion of Brain Tumor Cell Lines

Abstract

Cancer is a debilitating disease that is on the increase in both developed and developing countries. Anticancer drugs are often expensive, have narrow spectrum of activities, and are associated with toxicities and side effects such as myelosuppression, immunosuppression, gastrointestinal disturbance, alopecia, skin toxicity, and hepatotoxicity. Plants have been the major source of anticancer drugs both in orthodox and traditional medicine. Many of the plants claimed by the traditional medicine practitioners (TMPs) to be effective in the treatment of cancer are yet to be evaluated scientifically. In this work, five medicinal plants used by TMPs in Borno State, Nigeria, were tested against two brain tumor cell lines. Ethanol extracts of Securidaca longepedunculata, Andira inermis subsp. rooseveltii, Annona senegalensis, Carissa edulis, and Parinari polyandra were used. U87 and U231 brain tumor cell lines were used for proliferation assay, U251 cell line was used for the invasion assay in collagen V coated inserts, and U87 cell line was used for the western blot detection of cleaved Poly-ADP-Ribose-Polymerase (PARP). The result revealed that all tested extracts significantly (p<0.05) inhibited the proliferation of U87 and U231 cell lines with the respective IC50 values ranging between 8 and 20 μg/ml for S. longepedunculata and 100 and 90 μg/ml for P. polyandra. The five extracts significantly (p<0.05) inhibited the invasion of U251 cell line at the concentration of 10 μg/ml (S. longepedunculata), 20 μg/ml (A. inermis), 50 μg/ml (A. senegalensis), 50 μg/ml (C. edulis), and 50 μg/ml (P. polyandra). Securidaca longepedunculata extract induced the cleavage of PARP. It was concluded that these medicinal plants have antiproliferative and anti-invasive activities and possess good prospects as source of anticancer agents especially S. longepedunculata which induced apoptosis in U87 cell line.

Application of Data Mining Technology in Software Intrusion Detection and Information Processing

“…By verifying the experimental data of the intrusion detection prototype experimental system based on data mining, Ngulde, S.I. et al compared and analyzed the algorithm of the single intrusion detection model.Experimental results further confirm that the data mining model based on association rules and decision tree proposed in this paper is effective, especially in reducing the false positive rate of obvious effect, and has strong practical application [14]. Battini, N. et al discussed the process of designing and implementing a network intrusion detection system based on data mining and used experiments to verify the prototype system of intrusion detection and to optimize and improve existing problems [15].…”

Immunomodulatory Effect of Ginsenoside Rb2 Against Cyclophosphamide-Induced Immunosuppression in Mice

“…Almost all chemotherapeutic medicines are toxic, and chemotherapy has substantial and often fatal adverse effects, such as severe nausea, myelosuppression, and immunosuppression (Ngulde et al, 2020).…”

Sonicated Extract from the Aril of Momordica Cochinchinensis Inhibits Cell Proliferation and Migration in Aggressive Prostate Cancer Cells

“…Terefore, it is possible that phenolic compounds are active constituents of sonicated extract for suppression of prostate cancer cell viability and migration. Tis hypothesis is supported by many works showing that phenolic compounds from plants have anticancer and antimetastatic activities, which have been tested in several cancers, including skin cancer [44,45], oral cancer [46], pancreatic cancer [47], breast cancer [51], lung cancer [52], prostate cancer [53], and brain tumor [54].…”