Activity against drug resistant‐tuberculosis strains of plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases

Abstract

Tuberculosis (TB) kills about 3 million people per year worldwide. Furthermore, TB is an infectious disease associated with HIV patients, and there is a rise in multidrug‐resistant TB (MDR‐TB) cases around the world. There is a need for new anti‐TB agents. The study evaluated the antimycobacterial activity of nine plants used in Mexican traditional medicine to treat tuberculosis and other respiratory diseases. Nasturtium officinale showed the best activity (MIC = 100 µg/mL) against the sensitive Mycobacterium tuberculosis. The following plants were active also but at 200 µg/mL: Citrus sinensis, Citrus aurantifolia, Foeniculum vulgare, Larrea tridentata, Musa acuminata and Olea europaea. Contrary to the above data, activity against drug‐resistant variants of M. tuberculosis was more evident, e.g. N. officinale was the most potent (MIC ≤ 100 µg/mL) against the four mono‐resistant variants tested; F. vulgare and O. europaea were active against all the resistant variants (MICs ≤ 100 µg/mL). The most susceptible variant was the isoniazid resistant, being inhibited by C. aurantifolia, C. sinensis and O. europaea (MIC = 25 µg/mL). These data point to the importance of biological testing of extracts against drug‐resistant M. tuberculosis isolates, and the bioguided assay of these extracts for the identification of lead compounds against MDR‐TB isolates. Copyright © 2007 John Wiley & Sons, Ltd.

Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method

“…There were many different reference to analyze the antituberculosis activity of plant extracts. In previous study, plants extract were considered active against M. tuberculosis if MIC was <100µg/mL (Borges-Argáez et al, 2007;Gautam et al, 2007;Okunade et al, 2004), <200µg/mL (Camacho-Corona et al, 2008;Tosun et al, 2004), < 1600µg/mL (Mohamad et al, 2011), ≤ 2048 µg/ml (Tekwu et al, 2012). In other paper, the antimicrobial activity of plant extracts was classified as significant (MIC ≤100µg/mL), moderate (100 < MIC < 625µg/mL), or weak (MIC > 625 µg/mL) (Kuete, 2010).…”

Natural Compounds from Mexican Medicinal Plants as Potential Drug Leads for Anti-Tuberculosis Drugs

“…However, we could not find the original source of the ethnobotanical information. In any case, the hexane extract of leaves from O. europaea was reported to exhibit a MIC= 25µg/mL against the isoniazidresistant variant of H37Rv (Camacho-Corona et al 2008); therefore it result the most potent of the extracts here reviewed.…”

“…The hexane extract of the fruit peel has been tested against isoniazid and ethambutol resistant M. tuberculosis strains in vitro, the MICs were 25 and 50 µg/mL. The hexane extract of C. aurantifolia fruit peel showed identical results with these strains (Camacho-Corona et al 2008).…”

Antitubercular and Phytochemical Investigation of Methanol Extracts of Medicinal Plants Used by the Samburu Community in Kenya

“…This was attributed to various chemical constituents of the extracts, such as sodium chloride and potassium chloride, salvadourea, salvadorine, saponins, tannins vitamin C, silica, and resin, as well as cyanogenic or lignan glycosides, alkaloids, terpenoids, and oleic and stearic acids [11]. Phytoalexins which tend to fall into several classes including terpenoids, glycosteroids and alkaloids have been found to exert antibacterial effects in previous studies [12]. They probably contributed to the observed activity of the medicinal plants studied.…”