Activity and abundance of methane-oxidizing bacteria on plants in experimental lakes subjected to different nutrient and warming treatments

Esposito, Chiara; Nijman, Thomas P. A.; Veraart, Annelies J.; Audet, Joachim; Levi, Eti E.; Lauridsen, Torben L.; Davidson, Thomas A.

doi:10.1016/j.aquabot.2022.103610

Cited by 8 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6). Littoral zones can have markedly different GHG dynamics to deeper zones due to shallower water having lower pressure (Wik et al, 2013), less time for CH4 oxidation (Bastviken et al, 2008) or abundant plants which influence a range of biogeochemical processes (Davidson et al, 2018;Esposito et al, 2023). It is therefore possible that littoral zone dynamics could cause these differences.…”

Section: Discussionmentioning

confidence: 99%

Temporary stratification promotes large greenhouse gas emissions in a shallow eutrophic lake

Davidson¹,

Søndergaard²,

Audet³

et al. 2023

Preprint

View full text Add to dashboard Cite

Abstract. Shallow lakes and ponds undergo frequent temporary thermal stratification. How this affects greenhouse gas (GHG) emissions is moot, with both increased and reduced GHG emissions hypothesised. Here, weekly estimation of GHG emissions were combined with high-resolution temperature and oxygen profiles of an 11 hectare shallow lake to investigate how thermal stratification shapes GHG emissions. There were three main stratification periods with profound anoxia in the bottom waters occurring quickly upon isolation from the atmosphere. Average diffusive emission of methane (CH4) and nitrous oxide (N2O) were larger and more variable in stratified phase, whereas carbon dioxide (CO2) was on average lower. CH4 ebullition was an order of magnitude greater in the stratified phase. In addition, there was a large efflux of CH4 and CO2 when the lake mixed after periods of extended (circa 14 days) thermal stratification. These two turnover events were estimated to have released the majority of the CH4 emitted between May and September. These results highlight the role of turnover emissions resulting from temporary thermal stratification and also the need high frequency measurements of GHG emission in order to accurately characterise emissions from these temporarily stratifying lakes.

show abstract

Section: Discussionmentioning

confidence: 99%

Temporary stratification promotes large greenhouse gas emissions in a shallow eutrophic lake

Davidson¹,

Søndergaard²,

Audet³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…For each mesocosm, we determined the percent coverage (%-coverage) of the water column filled with macrophytes each week (Esposito et al, 2023). To do so, we photographed the side profile of each mesocosm, which included a scale for height for analysis in ImageJ (Rasband, 1997(Rasband, -2018.…”

Section: Plant Growth and Coveragementioning

confidence: 99%

Submersed Macrophyte Density Regulates Aquatic Greenhouse Gas Emissions

Theus,

Ray,

Bansal

et al. 2023

JGR Biogeosciences

View full text Add to dashboard Cite

Shallow freshwater ecosystems emit large amounts of greenhouse gases (GHGs), such as carbon dioxide (CO2) and methane (CH4), yet emissions are highly variable. The role that aquatic macrophytes play in regulating aquatic GHG emissions is uncertain despite their ability to dominate shallow waterbodies. Here, we studied the effects of submersed macrophyte (Ceratophyllum demersum) density on CO2 and CH4 concentrations and fluxes. We conducted a 61‐days experiment using mesocosms containing one of the following C. demersum density treatments: 0, 10, 20, or 30 individual shoots (n = 3). We found that high density C. demersum had the highest CO2 and CH4 surface water concentrations and emissions while there was no significant difference in CH4 in the low and medium densities and no plant control. The high density treatment lost biomass over the course of the experiment, indicating die‐off and additions of organic matter to the sediment. High organic matter loading and low dissolved oxygen likely stimulated GHG production in the high density treatment. Our results emphasize that submersed macrophyte density and periods of growth and dieback are important in regulating GHG emissions, which may help explain why shallow waterbodies are high yet variable sources of GHGs to the atmosphere.

show abstract

“…MOB oxidizes CH 4 when it diffuses into the water column during lake overturn, preventing CH 4 emission from lakes ( Bastviken et al, 2011 ; Esposito et al, 2023 ), which makes them essential mitigators of CH 4 release from lakes’ deeper layers ( Trotsenko and Murrell, 2008 ; Mayr et al, 2020 ; Ming et al, 2022 ). MOB communities also facilitate benthic-pelagic carbon intake and sequestration in aquatic food webs ( Bastviken et al, 2003 ; Deines and Fink, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

The methane-oxidizing microbial communities of three maar lakes in tropical monsoon Asia

Bicaldo,

Padilla,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Methane-oxidizing bacteria (MOB) is a group of planktonic microorganisms that use methane as their primary source of cellular energy. For tropical lakes in monsoon Asia, there is currently a knowledge gap on MOB community diversity and the factors influencing their abundance. Herewith, we present a preliminary assessment of the MOB communities in three maar lakes in tropical monsoon Asia using Catalyzed Reporter Deposition, Fluorescence In-Situ Hybridization (CARD-FISH), 16S rRNA amplicon sequencing, and pmoA gene sequencing. Correlation analysis between MOB abundances and lakes’ physicochemical parameters following seasonal monsoon events were performed to explain observed spatial and temporal patterns in MOB diversity. The CARD-FISH analyses detected the three MOB types (I, II, and NC10) which aligned with the results from 16S rRNA amplicons and pmoA gene sequencing. Among community members based on 16S rRNA genes, Proteobacterial Type I MOB (e.g., Methylococcaceae and Methylomonadaceae), Proteobacterial Type II (Methylocystaceae), Verrucomicrobial (Methylacidiphilaceae), Methylomirabilota/NC10 (Methylomirabilaceae), and archaeal ANME-1a were found to be the dominant methane-oxidizers in three maar lakes. Analysis of microbial diversity and distribution revealed that the community compositions in Lake Yambo vary with the seasons and are more distinct during the stratified period. Temperature, DO, and pH were significantly and inversely linked with type I MOB and Methylomirabilota during stratification. Only MOB type I was influenced by monsoon changes. This research sought to establish a baseline for the diversity and ecology of planktonic MOB in tropical monsoon Asia to better comprehend their contribution to the CH4 cycle in tropical freshwater ecosystems.

show abstract

Activity and abundance of methane-oxidizing bacteria on plants in experimental lakes subjected to different nutrient and warming treatments

Cited by 8 publications

References 49 publications

Temporary stratification promotes large greenhouse gas emissions in a shallow eutrophic lake

Temporary stratification promotes large greenhouse gas emissions in a shallow eutrophic lake

Submersed Macrophyte Density Regulates Aquatic Greenhouse Gas Emissions

The methane-oxidizing microbial communities of three maar lakes in tropical monsoon Asia

Contact Info

Product

Resources

About