Activity and isoenzyme profile of N-acetyl-β-d-glucosaminidase in urine from workers exposed to cadmium

Tassi, C; Abbritti, Giuseppe; Mancuso, Francesca; Morucci, P; Feligioni, L; Muzi, Giacomo

doi:10.1016/s0009-8981(00)00276-x

Cited by 17 publications

(20 citation statements)

References 25 publications

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“…These results agree with previous reports. [26][27][28][29][30][31] However, the activity of NAG A isoenzyme in urine of normal pregnant women was also significantly higher compared with urine of controls (33.1 ± 5.5 U/mmol Cr vs. 9.1 ± 1.8 U/mmol Cr; ratio 3.6), while NAG/NAG A ratio (an index of the contribution of NAG B to NAG activity) was significantly lower in urine of normal pregnant women with respect to that of controls.…”

Section: Discussionmentioning

confidence: 99%

“…24 Previous studies on isoenzymatic profile, evaluated by ion-exchange chromatography, of NAG from various biological sources showed that NAG A activity, evaluated by MUGS, is quantitatively lower than that measured by MUG and is a variable proportion of NAG activity. [25][26][27] The possibility to evaluate the different isoenzymatic profile of NAG has led to the notion of the so-called functional enzymuria mainly linked to preferential urinary excretion of A isoenzyme and the so-called lesional enzymuria with preferential urinary release of the B isoform. 28,29 However, little is known about the isoenzymatic pattern of urinary NAG during normal uncomplicated pregnancy.…”

Section: Introductionmentioning

confidence: 99%

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Isoenzyme A and UrinaryN-Acetyl-β-D-Glucosaminidase Activity in Normal Pregnancy

2011

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Objectives: Urinary N-acetyl-β-D-glucosaminidase (NAG) activity has been found to increase during normal uncomplicated pregnancy and such behavior could limit the diagnostic value of this enzyme for detection of subclinical tubular injury. The aim of this study was to evaluate urinary NAG activity and isoenzyme A in normal pregnant women at 30th week of pregnancy and in healthy women, to discriminate between physiological and lesional enzymuria. Design and methods: Enzyme activities in first morning fasting urine samples from 20 nonpregnant control and 20 normal pregnant women at 30th gestational week were evaluated by fluorometric methods. Results: Both total and isoenzyme A activity was significantly higher ( p < 0.01) in urines of normal pregnant women compared with control urines, whereas ratio between these two parameters was significantly lower ( p < 0.001). Conclusions: The increase of urinary NAG activity during normal uncomplicated pregnancy appears to be characterized by a prevalent increase in isoenzyme A form, a finding associated with functional (not lesional) enzymuria. The fluorometric assays may represent a simple and rapid method to evaluate whether increase in urinary NAG activity represents a renal physiological adaptation during pregnancy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isoenzyme A and UrinaryN-Acetyl-β-D-Glucosaminidase Activity in Normal Pregnancy

2011

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“…Others use HEX for monitoring chronic Cd exposure of factory workers, e.g. where particular pigments are used or Cd batteries are produced [22].…”

Section: Body Fluid Exoglycosidases As Disease Markersmentioning

confidence: 99%

Exoglycosidase markers of diseases

Chojnowska

Kępka

Szajda

et al. 2011

Biochemical Society Transactions

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Exoglycosidases are hydrolases involved in lysosomal degradation of oligosaccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). In tissues and body fluids, a higher exoglycosidase specific activity is found in N-acetyl-β-hexosaminidase, than β-glucuronidase, α-L-fucosidase, β-galactosidase, α-mannosidase and α-glucosidase. Determination of exoglycosidases (especially N-acetyl-β-hexosaminidase and β-glucuronidase) in body fluids could be an inexpensive, easy to perform and sensitive test for pathological evaluation, as well as in screening and monitoring many diseases, including alcohol abuse, risk of arteriosclerosis, bacterial infections (e.g. Lyme borreliosis), chronic inflammatory processes, such as rheumatoid arthritis and juvenile idiopathic arthritis, asthma, autoimmune hepatitis and primary biliary cirrhosis, as well as cancers.

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“…In proximal convoluted tubules HEX activity is three times higher than in renal corpuscles (Jung et al, 1992). HEX A and HEX B with the distinct dominance of HEX A are present in renal medulla and renal cortex (Tassi et al, 2000). In the urine of healthy humans the activity of HEX is low, mainly due to HEX A 97 derived from naturally exfoliated proximal convoluted tubule cells.…”

Section: Hex -Most Active Exoglycosidase In Catabolism Of Glycoconjugmentioning

confidence: 99%

Urinary Fructose-1,6-Bisphosphatase (FBP-1,6) and N-Acetyl-β-Hexosaminidase (HEX) in Monitoring Kidney Transplantation - Literature Review

Kępka¹,

Szajda²,

Waszkiewicz³

et al. 2011

Kidney Transplantation - New Perspectives

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Activity and isoenzyme profile of N-acetyl-β-d-glucosaminidase in urine from workers exposed to cadmium

Cited by 17 publications

References 25 publications

Isoenzyme A and UrinaryN-Acetyl-β-D-Glucosaminidase Activity in Normal Pregnancy

Isoenzyme A and UrinaryN-Acetyl-β-D-Glucosaminidase Activity in Normal Pregnancy

Exoglycosidase markers of diseases

Urinary Fructose-1,6-Bisphosphatase (FBP-1,6) and N-Acetyl-β-Hexosaminidase (HEX) in Monitoring Kidney Transplantation - Literature Review

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