Activity Correlation Imaging: Visualizing Function and Structure of Neuronal Populations

Junek, Stephan; Chen, Tsai Wen; Alevra, Mihai; Schild, Detlev

doi:10.1016/j.bpj.2008.12.3962

Cited by 49 publications

(76 citation statements)

References 27 publications

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“…The data were analyzed using custom written programs in MATLAB (Mathworks, Natick, USA). To facilitate selection of regions of interest, a "pixel correlation map" was obtained (see [24]). The fluorescence changes for individual ORNs are given as ΔF/F values.…”

Section: Calcium Imaging and Data Evaluationmentioning

confidence: 99%

Coordinated shift of olfactory amino acid responses and V2R expression to an amphibian water nose during metamorphosis

Syed

Sansone

Hassenklöver

et al. 2016

Cell. Mol. Life Sci.

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All olfactory receptors identified in teleost fish are expressed in a single sensory surface, whereas mammalian olfactory receptor gene families segregate into different olfactory organs, chief among them the main olfactory epithelium expressing ORs and TAARs, and the vomeronasal organ expressing V1Rs and V2Rs. A transitional stage is embodied by amphibians, with their vomeronasal organ expressing more 'modern', later diverging V2Rs, whereas more 'ancient', earlier diverging V2Rs are expressed in the main olfactory epithelium.During metamorphosis the main olfactory epithelium of Xenopus tadpoles transforms into an air-filled cavity (principal cavity, air nose), whereas a newly formed cavity (middle cavity) takes over the function of a water nose. We report here that larval expression of ancient V2Rs is gradually lost from the main olfactory epithelium as it transforms into the air nose.Concomitantly, ancient v2r gene expression begins to appear in the basal layers of the newly forming water nose. We observe the same transition for responses to amino acid odorants, consistent with the hypothesis that amino acid responses may be carried by V2R receptors.3

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Section: Calcium Imaging and Data Evaluationmentioning

confidence: 99%

Coordinated shift of olfactory amino acid responses and V2R expression to an amphibian water nose during metamorphosis

Syed

Sansone

Hassenklöver

et al. 2016

Cell. Mol. Life Sci.

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“…Two reference traces were taken from regions of interest in the recorded volume, one responding exclusively to temperature drops, the other one, to histidine only. Activity correlation imaging (ACI) 6 was computed based on the selected reference traces to visualize with high contrast the dendritic morphology of the postsynaptic networks corresponding either to the temperature or the histidineresponsive Ca 2+ signal (Figure 3C, D). Finally, thermosensitive and chemosensitive maps were color-coded and overlaid on top of each other,…”

Section: Representative Resultsmentioning

confidence: 99%

“…Finally, the imaging itself is done by line-illumination microscopy allowing the acquisition of 3D volumes. Lineillumination microscopy is one of the confocal techniques providing the highest possible acquisition rates 6 which are necessary to cover a large fraction of the olfactory bulb. Slower acquisition systems may be used but have the disadvantage that the size of the recorded volume must be reduced.…”

Section: Discussionmentioning

confidence: 99%

“…Note: Suitable setups include for example line-illumination microscopes scanning the sample line-wise instead of point by point. A simple realization of such a setup has been previously described 6 . Other options are spinning disk microscopes.…”

Section: Measurement Settingsmentioning

confidence: 99%

“…Note: Recordings with too much movement can be rescued by applying a shift correction procedure described in Kludt et al Note: Follow the steps described in Junek et al 6 as an alternative to using the provided MATLAB script.…”

Section: Image Processing Using Activity Correlation Imaging (Aci)mentioning

confidence: 99%

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Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

Brinkmann

Okom

Kludt

et al. 2016

JoVE

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The olfactory system, specialized in the detection, integration and processing of chemical molecules is likely the most thoroughly studied sensory system. However, there is piling evidence that olfaction is not solely limited to chemical sensitivity, but also includes temperature sensitivity. Premetamorphic Xenopus laevis are translucent animals, with protruding nasal cavities deprived of the cribriform plate separating the nose and the olfactory bulb. These characteristics make them well suited for studying olfaction, and particularly thermosensitivity. The present article describes the complete procedure for measuring temperature responses in the olfactory bulb of X. laevis larvae. Firstly, the electroporation of olfactory receptor neurons (ORNs) is performed with spectrally distinct dyes loaded into the nasal cavities in order to stain their axon terminals in the bulbar neuropil. The differential staining between left and right receptor neurons serves to identify the γ-glomerulus as the only structure innervated by contralateral presynaptic afferents. Secondly, the electroporation is combined with focal bolus loading in the olfactory bulb in order to stain mitral cells and their dendrites. The 3D brain volume is then scanned under line-illumination microscopy for the acquisition of fast calcium imaging data while small temperature drops are induced at the olfactory epithelium. Lastly, the post-acquisition analysis allows the morphological reconstruction of the thermosensitive network comprising the γ-glomerulus and its innervating mitral cells, based on specific temperatureinduced Ca 2+ traces. Using chemical odorants as stimuli in addition to temperature jumps enables the comparison between thermosensitive and chemosensitive networks in the olfactory bulb.

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Detection of silent cells, synchronization and modulatory activity in developing cellular networks

Hjorth

Dawitz

Kroon

et al. 2015

Developmental Neurobiology

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Developing networks in the immature nervous system and in cellular cultures are characterized by waves of synchronous activity in restricted clusters of cells. Synchronized activity in immature networks is proposed to regulate many different developmental processes, from neuron growth and cell migration, to the refinement of synapses, topographic maps, and the mature composition of ion channels. These emergent activity patterns are not present in all cells simultaneously within the network and more immature "silent" cells, potentially correlated with the presence of silent synapses, are prominent in different networks during early developmental periods. Many current network analyses for detection of synchronous cellular activity utilize activity-based pixel correlations to identify cellular-based regions of interest (ROIs) and coincident cell activity. However, using activity-based correlations, these methods first underestimate or ignore the inactive silent cells within the developing network and second, are difficult to apply within cell-dense regions commonly found in developing brain networks. In addition, previous methods may ignore ROIs within a network that shows transient activity patterns comprising both inactive and active periods. We developed analysis software to semi-automatically detect cells within developing neuronal networks that were imaged using calcium-sensitive reporter dyes. Using an iterative threshold, modulation of activity was tracked within individual cells across the network. The distribution pattern of both inactive and active, including synchronous cells, could be determined based on distance measures to neighboring cells and according to different anatomical layers.

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Activity Correlation Imaging: Visualizing Function and Structure of Neuronal Populations

Cited by 49 publications

References 27 publications

Coordinated shift of olfactory amino acid responses and V2R expression to an amphibian water nose during metamorphosis

Coordinated shift of olfactory amino acid responses and V2R expression to an amphibian water nose during metamorphosis

Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of <em>Xenopus laevis</em>

Detection of silent cells, synchronization and modulatory activity in developing cellular networks

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