Activity guided isolation of chemical constituents from the biologically active methanol extract of Euphorbia schimperi C. Presl

Abstract

ABSTRACT. In this study we investigated the chemical constituents of bioactive methanol extract of Euphorbia schimperi C. Presl. For this the methanol extract was fractionated into 20, 40, 60, 80% MeOH in CHCl3, and 100% MeOH fractions respectively by vacuum liquid chromatography. Excision wound surface of the animals were topically treated with these fractions at a dose of 100 mg/kg body weight for twenty days. Povidone-iodine ointment was used as a reference drug. Wound contraction measurement and period of epithelialization were used to assess the effect of fractions on wound repairing. The 100% MeOH fraction treated animals achieved significant (p < 0.001) value by showing 100% wound contraction and minimum period of epithelization (17.75±0.47) on the 20 th day as compared to standard drug treated animals on the same day. The active 100% MeOH fraction was subjected to various chromatographic techniques led to the isolation of miquelianin (1), kaempferol 3-O-glucuronide (2) and quercitrin (3). Compounds (1-3) were isolated from this plant for the first time.

Antidiabetic, antioxidant, molecular docking and HPTLC analysis of miquelianin isolated from Euphorbia schimperi C. Presl

“…Compounds 1 , 2 and 3 (standards) were isolated from E. schimperi (Ahmed et al, 2017). Analytical grade reagents such as ABTS, DPPH, p -anisaldehyde, acarbose, α‐Amylase, α‐glucosidae and BHA were purchased from Sigma Aldrich.…”

“…The active fraction leads to the isolation of a major compound quercetin-3- O -glucuronide (Compound 1) along with two minor compounds kaempferol-3- O -glucuronide (compound 2 ) and quercetin-3- O -rhamnoside (compound 3) (Fig. 1) using different chromatographic techniques (Ahmed et al, 2017). Kaempferol 3- O -glucuronide and quercetin-3- O -rhamnoside have also been reported from E. guyoniana and E. hirta (Smara et al, 2014, Mallavadhani et al, 2002).…”

Medicinal plants used for dermatological disorders among the people of the kingdom of Saudi Arabia: A narrative review

“…It is indigenous to Saudi Arabia's southern region, where it may reach a height of 1.7 m in height. It is known as “Al-dehin” in the Wadi Gama region of Saudi Arabia, where the milky latex collected from the plant is used in traditional medicine practice to cure cavernous stinking wounds ( Ali et al, 2017 , Ahmed et al, 2017 ). The plant is native to the Arabian Peninsula.…”

Novel Anti-Hepatitis B Virus Activity of Euphorbia schimperi and Its Quercetin and Kaempferol Derivatives

“…Euphorbia schimperi C. Presl. has been characterized for its various in vitro biological activities such as anti-cell proliferative, free-radical scavenging, and anti-microbial potential. , We have recently reported the in vivo wound healing property of the methanol extract of E. schimperi grown in Saudi Arabia . Several biologically active chemical constituents such as luteolin, scopoletin, kaempferol, flavonoid glycosides, and triterpenoids have been isolated from E.…”

“…21,22 We have recently reported the in vivo wound healing property of the methanol extract of E. schimperi grown in Saudi Arabia. 23 Several biologically active chemical constituents such as luteolin, scopoletin, kaempferol, flavonoid glycosides, and triterpenoids have been isolated from E. schimperi. 21,24 Previously, extracts of E. cotinifolia, E. cestrifolia, and E. tirucalli grown in Latin America have shown in vitro anti-herpes simplex virus (HSV) activities.…”