Activity-Guided Isolation of Constituents of <i>Tephrosia purpurea</i> with the Potential to Induce the Phase II Enzyme, Quinone Reductase

Chang, Leng Chee; Gerhäuser, Clarissa; Song, Lynda L.; Farnsworth, N. R.; Pezzuto, John M.; Kinghorn, A. Douglas

doi:10.1021/np970236p

Cited by 93 publications

(70 citation statements)

References 17 publications

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“…Three novel fl avonoids, (+)-tephrorins A and B and (+)-tephrosone, were isolated also isolated from T. purpurea. [24,25] One of the deguelins, tephrosin, is poisonous to fi sh, but not to mammals. The leaves contain up to 2.5% rutin.…”

Section: Introductionmentioning

confidence: 99%

Comparative toxicity assessment of three Tephrosia species on Artemia salina and animal cell lines

Subhadra¹,

Ravindran²,

Chintala³

et al. 2011

J Nat Pharm

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Background: Tephrosia calophylla, Tephrosia maxima and Tephrosia purpurea are generally used in the treatment of various ailments including cancer by the folklore of Indian subcontinent. Materials and Methods: The powdered root of three plants were separately extracted with chloroform and then subjected to preliminary chemical screening. The total phenolic and fl avonoid content were estimated by using gallic acid and quercetin as standard. Brine shrimp hatchability and lethality tests were done by hatching the cysts of Artemia salina in brine solution. As these parameters can be associated to tumor cells, the investigation was extrapolated to animal cell lines, Daltons Lymphoma Ascites (DLA) and Erlisch Ascites Carcinoma (EAC). Trypan blue exclusion method was adopted for this screening. In all experiments dose selected were between the ranges of 10 and 200 μg/mL. Results: Preliminary chemical screening revealed the presence of carbohydrates, proteins, alkaloids, tannins and fl avonoids for T. calophylla and T. maxima; proteins, alkaloids, tannins and fl avonoids for T. purpurea. The total phenolic and fl avonoid content was found to be highest in the case of T. calophylla followed by T. maxima and then by T. purpurea. It was observed that in all the cytotoxicity tests performed T. calophylla was proved to be most potent followed by T. maxima and then by T. purpurea. The results thus obtained were found to correlate with the values obtained for total phenolic and fl avonoid content. Conclusion: The present investigation revealed that all three species of Tephrosia to be potent cytotoxic agents. Increasing order of cytotoxicity can be denoted as T.purpurea

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Section: Introductionmentioning

confidence: 99%

Comparative toxicity assessment of three Tephrosia species on Artemia salina and animal cell lines

Subhadra¹,

Ravindran²,

Chintala³

et al. 2011

J Nat Pharm

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“…Phytochemical investigations on Tephrosia purpurea have revealed the presence of glycosides such as rutin, quercetin, and osyritin; retenoids such as deguelin, elliptone, rotenone and tephrosin; flavanoids such as lanceolatin A, B, C, purpurin, purpurenone, purpuritenin, and sterols such as ␤-sitosterol (Gokhale and Saraf, 2000). An isoflavone, 7,4 -dihydroxy-3 ,5 -dimethoxyisoflavone, and a chalcone, (+)-tephropurpurin, are also reported to be present in Tephrosia purpurea (Chang et al, 1997). The LD 50 of the ethanolic extract of the aerial parts of Tephrosia purpurea is 5.12 g/kg (Gokhale and Saraf, 2000).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of hepatoprotective activity of aerial parts of Tephrosia purpurea L. and stem bark of Tecomella undulata

Khatri

Garg²,

Agrawal

2009

Journal of Ethnopharmacology

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“…Diversas atividades biológicas já foram relatadas na literatura para as substâncias isoladas, tais como antimicrobiana e citotóxica para as substâncias 1 e 2, 6,13,14,20 e sedativa e anti-hiperglicêmica para a substância 2.…”

Section: Resultsunclassified

Toxicidade e atividade antioxidante de flavonoides das cascas das raízes de Lonchocarpus filipes

Santos¹,

Costa²,

Marques³

et al. 2009

Quím. Nova

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Recebido em 17/3/08; aceito em 24/5/09; publicado na web em 6/11/09 TOXICITY AND ANTIOXIDANT ACTIVITY OF FLAVONOIDS FROM Lonchocarpus filipes ROOT BARK. The phytochemical investigation of dichloromethane extract from root bark of Lonchocarpus filipes Benth (Leguminosae) afforded four flavonoids including three dibenzoylmethane derivatives rarely found in nature. The structures were established based on their spectral data ( 1 H and 13 C NMR, 2D-NMR) as being: lanceolatin B (1), pongamol (2), (E)-7-O-methylpongamol (3) and (E)-9-O-methylpongamol (4). Compound (4) is described herein for the first time as a natural product. The extracts and the isolated compounds (1), (2) and (3) displayed high toxicity in the brine shrimp lethality assay. Only compound (2) showed antioxidant activity using a DPPH radical scavenging assay. This is the first report on the phytochemical study of Lonchocarpus filipes.Keywords: Lonchocarpus filipes; flavonoids; dibenzoylmethanes. INTRODUÇÃOO gênero Lonchocarpus Kunth (Leguminosae), conhecido comumente como "Timbó", compreende cerca de 120 espécies, das quais 23 são nativas do Brasil.1 Várias espécies desse gênero são utilizadas na medicina popular como, por exemplo, as raízes e caules de L. chrysophyllus, L. martynii e L. spruceanus no tratamento de tumores, AIDS, eczema, dores de cabeça e doenças de pele.2 As raízes dessas espécies e as de L. urucu e L. utilis também são consideradas tóxicas para insetos e peixes, fato atribuído à presença da rotenona. 2,3Estudos prévios em espécies de Lonchocarpus revelaram a presença de flavonoides, triterpenos, derivados de ácido benzoico, aminoácidos e alcaloides, 4,5 sendo os flavonoides considerados os constituintes predominantes em espécies desse gênero. [6][7][8] Os flavonoides são considerados um dos maiores grupos de metabólitos secundários e têm se destacado por apresentarem diversas propriedades farmacológicas, agindo como antitumorais, anti-inflamatórios, antioxidantes, antivirais, fungicidas, antiprotozoários, bem como na redução de riscos de doenças cardiovasculares. 8,9 O gênero Lonchocarpus é subdividido em dois subgêneros: Lonchocarpus subgênero Punctati o qual não está subdividido em seções, e Lonchocarpus subgênero Lonchocarpus, subdividido em cinco seções: Laxiflori, Unguiflori, Pubiflori, Lonchocarpus e Densiflori. 10 De acordo com essa classificação botânica, Lonchocarpus filipes Benth pertence ao subgênero Lonchocarpus, seção Laxiflori, ao lado de L. campestris. 11Lonchocarpus filipes é uma árvore tipicamente brasileira, variando de 2 a 4 m de altura, com distribuição restrita ao estado do Rio de Janeiro. É encontrada em matas e bosques da capital, estendendo-se até Petrópolis.10 Atualmente é cultivada no Parque Ecológico da Universidade Estadual de Campinas (UNICAMP).Neste trabalho são descritos o isolamento, a avaliação da toxicidade e da atividade antioxidante dos extratos e substâncias isoladas das cascas das raízes de L. filipes. Este é o primeiro relato de estudo fitoquímico dessa espécie. RESULTADOS E DISCUSSÃOO fracion...

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Activity-Guided Isolation of Constituents of Tephrosia purpurea with the Potential to Induce the Phase II Enzyme, Quinone Reductase

Cited by 93 publications

References 17 publications

Comparative toxicity assessment of three Tephrosia species on Artemia salina and animal cell lines

Comparative toxicity assessment of three Tephrosia species on Artemia salina and animal cell lines

Evaluation of hepatoprotective activity of aerial parts of Tephrosia purpurea L. and stem bark of Tecomella undulata

Toxicidade e atividade antioxidante de flavonoides das cascas das raízes de Lonchocarpus filipes

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