Activity‐guided isolation of α‐amylase, α‐glucosidase, and pancreatic lipase inhibitory compounds from Rhus coriaria L

Abstract

The leaves and fruits of Rhus coriaria are traditionally used in Turkey for the treatment of diabetes. The aim of the present study is to determine α-amylase, α-glucosidase, and pancreatic lipase inhibitory activities of R. coriaria leaf and fruit ethanol extracts (80%), and to isolate active compounds against these enzymes. As a result of the activity-guided isolation, the active compounds were determined as the amentoflavone, agathisflavone, and 1,2,3,4,6-penta-O-galloyl-βglucopyranose. Agathisflavone, amentoflavone, and penta-O-galloyl-β-glucopyranose inhibited α-glucosidase with 11.4 ± 0.9, 11.3 ± 0.7, and 4.1 ± 0.1 μM IC 50 values, respectively. Furthermore, penta-O-galloyl-β-glucopyranose inhibited αamylase with 6.32 ± 0.18 μM IC 50. These three compounds also significantly inhibited (P < 0.05) pancreatic lipase. The results of high-performance liquid chromatography analysis showed that penta-O-galloyl-β-D-glycopyranose was one of the main compounds in both fruit and leaf extracts. Therefore, it may be considered that R. coriaria fruit and leaf extracts can be standardized on this substance and used in the development of both medicinal products and functional food for diabetes.

Phytochemical Profiling and Assessment of Antidiabetic Activity of Curcuma Angustifolia Rhizome Methanolic Extract: An In Vitro and In Silico Analysis

“…The inhibition of carbohydrate hydrolyzing enzymes, particularly α‐amylase and α‐glucosidase, which catalyzes the hydrolysis of carbohydrates and causes higher blood glucose levels is a potential therapeutic strategy in the treatment of T2DM [34,35] . Inhibition of these enzymes may improve glycemia.…”

“…The inhibition of carbohydrate hydrolyzing enzymes, particularly α-amylase and α-glucosidase, which catalyzes the hydrolysis of carbohydrates and causes higher blood glucose levels is a potential therapeutic strategy in the treatment of T2DM. [34,35] Inhibition of these enzymes may improve glycemia. [34] Based on the percentage of inhibition, the inhibition activity of the methanolic extract of C. angustifolia rhizome on α-glucosidase had higher potential than α-amylase.…”

Investigation of Antidiabetic Effect of Pistacia atlantica Leaves by Activity-Guided Fractionation and Phytochemical Content Analysis by LC-QTOF-MS

“…The most active EtOAc subextract was standardized for methyl gallate, which was found to be the major compound. Methyl gallate was previously isolated from the leaves of Rhus coriaria L. (Gök et al, 2020). For the analysis, HP Agilent 1260 series LC System and Agilent TC-C18 170Å (4.6 mm × 150 mm × 5 µm) column were used.…”

Metabolic regulation and antihyperglycemic properties of diet-derived PGG through transcriptomic and metabolomic profiling

“…13 PGG can also exert inhibitory effects on α-amylas and pancreatic lipase. 14 Diabetes can cause significant changes in the metabolic profile. The amino acids, carbohydrates, nucleotides, and xenobiotics pathways showed the most notable perturbations in individuals with type 2 diabetes, while the lipid pathways presented the most significant perturbations in those with triglycerides.…”