Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions

Leyva, Luis A.; Bashan, Yoav; de‐Bashan, Luz E.

doi:10.1007/s13213-014-0866-3

Cited by 28 publications

(11 citation statements)

References 58 publications

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“…At this stage, the activities of the microalgal enzymes (two were tested so far, glutamine synthetase and glutamate dehydrogenase) are not enhanced (de-Bashan et al 2008c ). At the next phase of interaction, beginning about 48 h after joint immobilization and continuing, glutamate synthetase and glutamate dehydrogenase activities are enhanced, photosynthetic pigment production is enhanced ( de-Bashan et al 2002a ), nitrogen and phosphorus uptake into microalgal organelles is accelerated (de-Bashan et al 2005 ), carbohydrates accumulation, especially starch, occurs (Choix et al 2012a , b ), as well as an increase in lipids and fatty acids (de-Bashan et al 2002a ;Leyva et al 2015 ). At the same time, the co-immobilized system liberates oxygen produced by Chlorella spp.…”

Section: Basic Studies Of Prokaryotic-eukaryotic Interactionmentioning

confidence: 99%

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

de‐Bashan¹,

Hernández²,

Bashan³

2015

Handbook for Azospirillum

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The interaction of the bacteria Azospirillum spp. with photosynthetic, single cell microalgae that are co-immobilized in alginate beads provides a significant shortcut for understanding the interaction of this plant growth-promoting bacteria (PGPB) with plants in general. This interaction is currently relevant for studying physiological, physical, biochemical, and molecular aspects. As an independent subfi eld of Azospirillum research, this interaction has some signifi cant potential biotechnological applications, such as wastewater treatment, production of biofuel (ethanol and biodiesel), increased fertility of eroded soils combined with promoting growth of higher plants, production of pigments, and production of biomass. All of these applications have yet to be scaled up and evaluated for their true practical potential. The Logic Behind Using This Interaction as a Model for Plant-Bacteria InteractionA major obstacle in the study of interactions between Azospirillum spp. and plants is the complexity of the plant. Studies of basic plant-bacterium interactions of Azospirillum spp., done mainly with roots, are diffi cult because there are manyDedication : This chapter is dedicated to the memory of the German/Spanish mycorrhizae researcher Dr. Horst Vierheilig (1964 of CSIC in Spain.

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Section: Basic Studies Of Prokaryotic-eukaryotic Interactionmentioning

confidence: 99%

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

de‐Bashan¹,

Hernández²,

Bashan³

2015

Handbook for Azospirillum

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“…Many strategies, such as co-immobilized with Azospirillum brasilense and nutrient starvation, have been investigated to increase lipid content of microalgae (Leyva et al, 2014a;Leyva et al, 2014b). Nitrogen starvation has proven an effective strategy for enhancing lipid content and has been commonly employed in autotrophic cultivation (Griffiths and Harrison, 2009;Rodolfi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Shen¹,

Chu²,

Lam

et al. 2015

Water Research

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“…This is consistent with the increase in the lipid content of algae cells. However, the different gene expression levels in the metabolic pathway of fatty acid elongation and unsaturated fatty acid biosynthesis also affect the components of the fatty acid chain, indicating that the response of algal cells to the high salt environment is complex and diverse [ 27 ]. The next experiment will continue to explore the impact of key genes on the length of fatty acid chains, through targeted control of gene expression levels to control fatty acid components.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous Chlorococcum sphacosum GD from Soil under Salt Stress

Feng

et al. 2021

IJMS

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The oleaginous microalgae species Chlorococcum sphacosum GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of Chlorococcum sphacosum GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of C. sphacosum GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway.

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Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions

Cited by 28 publications

References 58 publications

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation

Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous Chlorococcum sphacosum GD from Soil under Salt Stress

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